Learn about the foundations of molecular cloning and recombinant DNA technology

Find resources suitable for researchers at multiple levels beginning with an overview of restriction enzymes as fundamental tools and their applications in common cloning and non-cloning research strategies.

Restriction enzymes

Find out about historical overview of restriction enzymes, usage considerations, troubleshooting, and non-cloning applications.

Cloning

Learn about the traditional cloning workflow, troubleshooting, PCR cloning, subcloning as well as library preparation, and shotgun sequencing.

Competent cells and transformation

Review competent cells, their usage, and tips for bacterial transformation in molecular cloning.

Molecular cloning videos

Additional technical resources

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