Applied Biosystems™

CytoScan™ HD Array Kit and Reagent Kit Bundle

Catalog number:  901835

 Related applications:

Microarray Analysis

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Description

CytoScan™ HD Suite includes CytoScan HD Array, CytoScan Reagent Kit, the easy-to-use Chromosome Analysis Suite (ChAS), and the proven GeneChip™ Instrument System.

CytoScan HD Suite is the unrivaled standard in cytogenetics research and delivers with:

Exceptional performance-coverage without compromise
CytoScan HD Suite provides the broadest coverage and highest performance for detecting chromosomal aberrations. CytoScan HD Suite has greater than 99% sensitivity and can reliably detect 25-50kb copy number changes across the genome at high specificity with single-nucleotide polymorphism (SNP) allelic corroboration. With more than 2.6 million copy number markers, CytoScan HD Suite covers all OMIM™ and RefSeq genes. Our proprietary manufacturing technology produces arrays that are highly reproducible between each batch with no risk of probe dropout inherent in bead manufacturing techniques. Surpassing the limitations of current database curations, the whole-genome array ensures that novel findings can be catalogued for future discoveries and annotations, which are often missed by targeted designs.

High-density SNPs with >99% genotype accuracy
Enables low-level mosaicism visualization, absence of heterozygosity (AOH) and acquired UPD (aUPD) detection, copy number change confirmation, triploidy detection, allelic imbalance pattern visualization, genomic contamination identification, trio consistency checking,and parent-of-origin analysis.

The broadest range of sample types supported - including POC FFPE
CytoScan HD Suite supports several different sample types for cancer and constitutional cytogenetic research analysis, including blood, bone marrow, buccal, saliva, fresh and frozen tissues, DBS cards, direct/cultured cells, amniocytes, and products of conception (POC), fresh and formalin-fixed, paraffin-embedded non-archived samples among others.

Robust manual or automated assay workflows-DNA to result in less than three days, including newly available CytoScan Amplification Kit
CytoScan HD Solution has been optimized to save laboratory time, money and resources. The simplified and streamlined assay protocol is aligned with laboratory workflow requirements, making it easier to obtain consistency, high-quality results, and reduce operator error. The reagent kit includes reagents and magnetic beads required for the CytoScan assay (except ethanol). Each component passes strict quality control inspection to ensure the highest reproducibility and performance with CytoScan HD Arrays. The CytoScan protocol can be accomplished in 2.5 days with just 7 hours of hands-on time.

CytoScan Amplification Kit is now available!
CytoScan Amplification Kit (Cat. No. 902975) has been designed to be used at the PCR amplification step of the CytoScan assay and is manufactured by Takara Bio USA, Inc (Clontech). CytoScan Amplification Kit is equivalent to the Clontech Titanium™ DNA Amplification Kit (Cat. No. 639240, 300 reactions, or Cat. No. 639243, 400 reactions). The Amplification reagents in the CytoScan Amplification Kit generate PCR products to be used in the preparation of the target DNA to be hybridized to CytoScan 750K and HD Arrays. The kit contains enough reagents to prepare 400 PCR reactions-equivalent to 96-100 samples processed with the CytoScan assay.

Affymetrix NIMBUS Target Preparation Instrument - automate your workflow
Affymetrix NIMBUS Target Preparation Instrument offers your lab the premier in compact liquid-handling instrumentation. The sleek Affymetrix NIMBUS workstation is optimized to automate the post-PCR portion of the CytoScan assay, including the DNA purification and pooling stages. CytoScan Automated Target Preparation Solution is ideal for labs looking to handle 24 or 48 samples with ease and increase sample throughput.

Chromosome Analysis Suite (ChAS)-designed for cytogenetic applications
The popular ChAS Software is available free of charge as part of the CytoScan HD Suite. ChAS is tailored to cytogenetic research analysis and reporting with:

• Streamlinedanalysis and reporting workflow
• Ability to apply customized filters to analyze the genome at different levels of resolution
• Options to create, modify, and upload annotation files and flag regions for focused analysis
• A database for storage, query and visualization of samples
• Direct access to externaldatabases such as NCBI, UCSC Genome Browser, Ensembl, and OMIM
•Trio consistency checking for parental studies
•Cancer-tailoredanalysis tools

First-class customer training and support
Customer training is required for the CytoScan HD assay for both new and existing customers. Different products are available based upon your experience level; please contact us here for details.

Related Links
905824 CytoScan™ HD Kit Plus 24
905896 CytoScan™ HD Kit Plus 96

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Array Format: Genechip probe array
Array Type: Cytogenetics & Copy Number
Number of Arrays: 24 arrays
Number of Markers: 2.67 million markers
Product Size: 1 kit

Documents

Manuals & protocols