Invitrogen™

Anza™ 11 EcoRI

Catalog number: IVGN0116
Invitrogen™

Anza™ 11 EcoRI

Catalog number: IVGN0116
Catalog Number
IVGN0116
also known as IVGN011-6
Unit Size
8000 units
Price (USD)
61.25
Your Price:
Availability
***
Quantity
Catalog Number
IVGN0118
also known as IVGN011-8
Unit Size
40,000 units
Price (USD)
242.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
IVGN0116
also known as IVGN011-6
8000 units
61.25
Your Price:
***
IVGN0118
also known as IVGN011-8
40,000 units
242.00
Your Price:
***
Product Overview
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Recommendations
5'  G ↓A  A  T  T  C   3' 
3'  C  T  T  A  A ↑G   5' 

Invitrogen™ Anza™ 11 EcoRI is a restriction enzyme that cuts DNA at this recognition site: G^AATTC , completely digesting the DNA in 15 minutes at 37°C.

For superior convenience, a single buffer and protocol are used with all Anza™ restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This helps eliminate the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.

Convenient—all Anza enzymes are 100% functional in the single Anza buffer
Fast—complete digestion in 15 minutes
Flexible—overnight digestion without star activity
Simple—a single digestion protocol for all DNA types
Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience

Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza Alkaline Phosphatase Kit, Anza T4 PNK Kit, Anza DNA Blunt End Kit, and Anza DNA End Repair Kit. Use these enzymes for a complete cloning workflow.

Anza Red Buffer, which contains a density gradient and tracking dyes, is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that require product analysis by fluorescence excitation, the colorless Anza Buffer without the tracking dyes is recommended.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity
8000 U
Product Line
Anza™
Shipping Condition
Approved for shipment on Wet or Dry Ice
Methylation Sensitivity
Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive
Enzyme
EcoR I
Compatible Buffer
Anza™ 10X Red Buffer, Anza™ 10X Buffer
Sensitive to Heat Inactivation
Yes
Optimal Reaction Temperature
37°C
Type IIS RE
No

Contents & Storage

400 µL Anza 11 EcoRI
1 mL Anza Buffer (10X)
1 mL Anza Red Buffer (10X)

Store at -20°C.

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Citations & References