Maxima SYBR Green/ROX qPCR Master Mix (2X)
Thermo Scientific™
Maxima SYBR Green/ROX qPCR Master Mix (2X)
**********************Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at aRead more
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Catalog NumberNo. of Reactions
K02221,000 Reactions
K0221200 reactions
K02234,000 Reactions
3 Options
Catalog number K0222
Price (USD)/ 1,000 reactions
946.65
Online exclusive
1064.00 
Save 117.35 (11%)
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Add to cart
No. of Reactions:
1,000 Reactions
Price (USD)/ 1,000 reactions
946.65
Online exclusive
1064.00 
Save 117.35 (11%)
Add to cart
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Catalog numberK0222
Price (USD)/ 1,000 reactions
946.65
Online exclusive
1064.00 
Save 117.35 (11%)
-
Add to cart
**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green/ROX qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodSYBR
FormatTube
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
PolymeraseTaq DNA Polymerase
Reaction SpeedStandard
For Use With (Equipment)7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Product LineMaxima
Product TypeReal Time PCR SYBR Master Mix
Sufficient For1000 Reactions at 25 μL
For Use With (Application)Gene Expression
Concentration2X
No. of Reactions1,000 Reactions
Sample TypeDNA, dsDNA
Volume10 x 1.25 mL
Unit Size1,000 reactions
Contents & Storage
Contains:
• 10 x 1.25 mL Maxima SYBR Green/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye; sufficient for 1000 x 25 μL reactions.
• 10 x 1.25 mL Nuclease-Free Water

Store at -20°C.