RT-PCR is a method-of-choice for revealing target gene expression in applications ranging from virology to next-generation sequencing. A one-step process with Invitrogen SuperScript IV UniPrime – featuring a universal annealing temperature and colorimetric buffers – can help you avoid five of the most common RT-PCR challenges.
The polymerase chain reaction (PCR) is one of the most well-known techniques in molecular biology. Vast improvements have been made to DNA polymerases, reagents, and instrumentation over the years. Have you kept up? Our new Tips & Tricks for PCR Guide infographic will help you stay up to date on the tried-and-true tips, cutting-edge tricks, and common hurdles you’d rather skip. Download the Tips & Tricks for PCR Success infographic guide.
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