The Thermo Scientific Velocity LFQ DIA platform is a premier quantitative proteomics platform that offers research laboratories uniquely accurate and precise quantitation, with deep proteome coverage and data completeness, for high-confidence characterization of the differences between biological systems. Over four orders of dynamic range enhances discovery of low-abundance proteins in complex biological samples. Based on HRAM Thermo Scientific Orbitrap mass spectrometer, Thermo Scientific Vanquish NEO UHPLC, and high-efficiency Thermo Scientific µPAC HPLC column technologies, the platform provides robust, high-throughput analysis of thousands of samples with the statistics needed to power conclusions, dramatically reducing time to biological insight for large scale studies. 

Uniquely accurate and precise quantitation

  • Determine correct protein abundance
  • Reduces number of measurements need to draw conclusions

High confidence with deep proteome coverage and data completeness

  • Direct DIA provides >99% data completeness
  • Spectronaut SW & library search for deeper proteome profiling for screening approaches

High throughput for large sample cohorts reduces time to biological insight

  • Provides statistical significance                                                                   
  • Vanquish Neo and uPAC columns provide high efficiency separations

Robust platform and method

  • Easy day-to-day platform operation for analyses of thousands of samples
  • Velocity LFQ HR-DIA platform customer testimonials

Wide dynamic range

  • Discover differentially expressed low-abundance proteins of biological significance
  • Confident quantitation of uncharacterized low-abundance proteins        

The Velocity LFQ DIA  platform is a premier quantitative proteomics system that uniquely delivers robust quantitative accuracy and precision, in combination with high proteome coverage and data completeness, for high-confidence, high-throughput characterization of large sample cohorts, for investigators in quantitative proteomics, translational research, biomarker discovery, and biopharma research labs, who need to characterize the differences between biological systems using precise quantitative measurements for hypothesis testing.

High-throughput high-resolution data-independent acquisition workflow for accurate label-free quantitation
Graphical schematic of DIA workflow

Graphical schematic of DIA workflow for label-free quantitation of two- and three-proteome mixtures. The different components of the workflow are depicted on the top. The main goal of the setup is the quantitative performance at high sample throughput while delivering robust and reproducible results to make it a perfect fit for large scale clinical and biomarker discovery studies.

Robust and reproducible workflow for accurately quantifying and identifying hundreds to thousands of proteins from complex sample mixtures with a high background of human peptides. The LC method and gradient were optimized to fully utilize the capabilities of micropillar array-based separation columns. Similarly, the mass spectrometric method was adopted to account for the short gradient lengths and high throughput without compromising identification rates at great quantitation accuracy and precision. The long-term robustness of the workflow is demonstrated by intermittent quality control runs.

1000 Orbitrap DIA Publications

Over one thousand peer-reviewed publications with Orbitrap mass spectrometers, covering very broad range of proteomic applications, including for example analysis of SARS-CoV-2 host proteins, protein functions, noninvasive proteomics biomarkers, drugs, protein shapes and proteogenomic subtypes of acute myeloid leukemia. A subset of the 1000 publications are highlighted below.

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