Extraordinary Simplified
Orbitrap Exploris Mass Spectrometer

Making genius simpler

Empowering life science researchers to translate each experimental step to a new level of insight with a next-generation ownership experience coupled with an intelligence-driven experimental approach. With the Thermo Scientific Orbitrap Exploris 480 mass spectrometer you'll find market leading quantitative performance to solve your most complex challenges with ease, and the proven qualitative performance you expect.

From exploratory to targeted quantitation, find the power and reliability to make large scale studies possible, while reducing everyday hassles as you increase productivity. And achieving it all, remarkably, within a compact footprint. An extraordinary instrument, providing extraordinary results.

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Democratizing the power of mass spectrometry with the Thermo Scientific Orbitrap Exploris 480 MS

With game-changing performance and an unparalleled user experience, the Thermo Scientific Orbitrap Exploris 480 mass spectrometer makes it possible for an expanded audience of molecular biologists and biochemists to see themselves as mass spectrometrists. You’ll have access to a vast range of pre-set experiments, which are customizable with simple drag and drop modifications, and the sensitivity and selectivity necessary to support emerging applications such as single cell proteomics.


Introducing the Orbitrap Exploris 480 Mass Spectrometer

Discover how the latest in instrument design delivers proven technology, advanced capabilities, a compact footprint, and an intelligence-driven analytical framework empowering research scientists to more extensively deploy mass spectrometry for rigorous, high-throughput protein identification, quantitation, and structural characterization. Genius just got simpler.



Key benefits of the Orbitrap Exploris 480 mass spectrometer

Next-generation experience High performance,
high-throughput insights
Robust performance, exceptional serviceability increase uptime Ease of use, intelligence empower staff
The Orbitrap Exploris 480 mass spectrometer joins the family of next-generation Thermo Scientific mass spectrometers built on a common architecture with the guiding principle of ultimate performance and ease of use. Adding more performance and intelligence to market-leading selectivity and spectral quality imparts productivity, depth, and certainty to small and large-scale studies. By design, exceptional end-to-end robustness minimizes need for performance-related maintenance, increasing uptime and sample throughput. Differences in user expertise can be a challenge in many laboratories. Workflows based on intelligent data acquisition methods leverage the new levels of instrument performance to deliver high confidence and high throughput.


Learn how the Orbitrap Exploris 480 MS works

See what makes the Orbitrap Exploris 480 MS stand out and how it can empower all users to obtain the highest quality results and deepest insights with the least amount of effort. Learn below how our breakthrough is now your breakthrough.

Explore how to achieve high throughput LCMS screening while maintaining protein depth and quantitative accuracy

Engineering feedback from customers and service engineers into the Orbitrap Exploris 480 MS was a top priority, leading to a complete instrument design from the ground up based around system usability, as well as technological advancements in pumping technology, control electronics and ion optics.

With a focus on robustness, reliability, performance and size, not only have we reduced the length of the quadrupole while maintaining ion transmission efficiency, we have included patented configuration switching meaning the quadrupoles stay cleaner for longer. In addition, we have improved the Orbitrap, C-Trap, ion-routing multipole and bent flatapole design, also including the source and funnel improvements from the Thermo Scientific Orbitrap Tribrid platform.

The highly optimized design permits a compact physical footprint while providing high performance components and many new functionalities that significantly advance performance for many critical application areas. We achieved a more compact footprint with a single turbo-pump with six-stage pumping, which allowed for fewer moving parts, less electricity consumption, noise and weight.

Ion path and key components of the Orbitrap Exploris 480 Mass Spectrometer

Ion path and key components of the Orbitrap Exploris 480 Mass Spectrometer

Introduction to Orbitrap Exploris 480 MS – Game changing performance, reliability and data certainty

Today's translational scientists need to go beyond peptide and protein identification. Learn how to achieve results in a variety of application areas, including protein profiling with FAIMS next generation ion mobility technology, quantitative protein analysis, and detailed protein characterization for translational research. Leveraging new design features, the system takes advantage of a multitude of novel ion-optical and instrument intelligence-driven design solutions that update and extend capabilities to deliver the performance, robustness, reliability, and data certainty beyond that of current high resolution mass spectrometers.

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Thermo Scientific SureQuant IS Targeted Protein Quantitation workflow is a data-aware quantitation scan mode leveraging internal standards to dynamically adjust scan parameters and automatically maximize data quality for targeted proteome analysis in real time. The SureQuant method fills the gap for large-scale target profiling with superior quantitative performance.

Certainty in Biological States of Proteins

SureQuant IS targeted protein quantitation

While triple quadrupole-based Selected Reaction Monitoring (SRM) and high-resolution Parallel Reaction Monitoring (PRM) approaches are different, each offers advantages to targeted quantitation. SureQuant IS targeted protein quantitation with Thermo Scientific Orbitrap mass analyzer-based mass spectrometry reduces the inevitable trade-off between multiplexing, sensitivity and selectivity. This method enlarges targeted protein panel sizes and significantly improves limits of detection (LODs) and quantitative accuracy without use of retention time windows.

SureQuant method for targeted protein quantitation

SureQuant method facilitates an intelligence-driven approach

SureQuant method target detection occurs independently of Retention Time (RT) scheduling, allowing reliable and robust target quantification. This intelligence-driven approach for quantitation enables self-adjustment for higher sensitivity without compromising cycle time.

Genius Made Simpler: Improving Everyday Protein Profiling to Quantitative Analysis

Regardless of your expertise, speed up your analysis and certainty. Learn how to take advantage of a multitude of novel ion-optical and instrument intelligence-driven design solutions that update and extend capabilities to deliver the performance and data certainty today's translation research labs should expect.

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New high-field Orbitrap mass analyzer doubles resolving power

Increased resolution and speed up to 480,000 resolution or 40Hz scan speed

Boost data quality with high-sensitivity, high selectivity quadrupoles

Increased mass range from m/z 40 up to m/z 6000; up to m/z 8000 when purchased with the BioPharma option.

Ion-Routing Multipole

Ion routing is via the multipole device, similar to our Orbritrap Tribrid mass spectrometers. It can also perform Higher Collisional Dissociation (HCD) fragmentation

Other high performance features

  • Thermo Scientific TurboTMT, powered by Φ-SDM algorithm offers faster TMT scanning to improve peptide detection and quantitative accuracy.
  • Precursor Fit filter allows for selecting ions based on precursor ion specificity of the isolation window.
  • BoxCar for DDA and DIA improves dynamic range for peptide detection.
  • Achieve Thermo Scientific SureQuant targeted quantitation with a SureQuant kit that enables an internal standard triggered method of quantitation which enlarges targeted protein panel size and significantly improves LODs and quantitative accuracy without use of retention time windows.
  • Increased scan method selection for TopN/TopSpeed DDA, DIA and MSX.

Advancing Quantitative Proteomics: Multiplexing, Accuracy, and Precision

Quantitative proteomics strategies using Tandem Mass Tags™ enable precise measurement of protein abundance from multiple samples into a single high-resolution LC-MS analysis. Increasingly, various biological experiments demand higher multiplexing and sample throughput with accurate quantitation. In this workshop, our expert speakers will lay out strategies that combine next generation technologies in multiplexing chemistry, hardware, and software to achieve increased quantitative performance and throughput efficiency

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Setting up your system to acquire the highest quality data is simple and more effortless with a consistent, common instrument control software approach covering our Thermo Scientific Orbitrap Tribrid MS, Thermo Scientific Orbitrap Exploris 480 MS, and Thermo Scientific TSQ triple quadrupole mass spectrometers.

Focus on your science, rather than experimental set-up, through ready-to-use application specific method templates for common experiments; method development can be eliminated or significantly reduced by using these templates, editing them or creating your own through drag-and-drop method set-up.

Designed for Experimental Parameters to be Transferable between Systems

Generating high-quality data is easier than ever before with drag-and-drop method-building and the ability to utilize ready-made templates for the most popular experiments. The common software architecture provides a consistent user experience, simplifying operation, training and streamlining transfer of knowledge throughout your workflows.

The Thermo Scientific Method Editor and Tune ecosystem deliver intuitive method programming with ready-to-go templates for the most popular experiments, such as Glycosylation, Metabolites, Peptide ID, Peptide Quantitation, PTM, Advanced TMT, SureQuant method and more.

MethEdit Orbitrap Exploris 480 Flow

The common method editor combines both instrument control and data acquisition parameters, through the use of extensive templates for the most common experiments to allow you to acquire high-quality data from the start, or use drag-and-drop to rapidly create and visualize your own experiments.


See the Orbitrap Exploris 480 MS in action: watch ion flight paths for:

  • Full Scan MS
  • MS/MS (ddMS2)
  • DIA – Data Independent Acquisition
    • Full Scan MS
    • MS/MS of m/z 100-200 (mass range)
    • MS/MS of m/z 200-300 (mass range)
    • MS/MS of m/z 300-400 (mass range)
    • MS/MS of m/z 500-600 (mass range)
    • MS/MS of m/z 600-700 (mass range)
  • tSIM
  • tSIM MSX


Optional features to maximize performance

Maximize performance and flexibility to expand experimental capabilities of the Orbitrap Exploris 480 mass spectrometer with multiple hardware and software options, allowing you to see more, quantify more, deliver more confidence and greater insights with even more productivity.

Thermo Scientific FAIMS Pro interface enhances precursor selectivity, improving qualitative and quantitative results for most peptide and protein applications while reducing time consuming sample preparation steps.

Identifying and characterizing proteins and post-translational modifications by bottom-up mass spectrometry relies on the acquisition of high quality MS and MS/MS data. The FAIMS Pro interface increases analytical performance through gas phase fractionation and selective enhancement of peptidic compounds, reducing the complexity of the MS spectra, and improving analyte signal/noise ratio. The end result is greater proteome coverage.

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FAIMS Pro Interface
Enabling high-throughput screening


Easy-IC Ion Source

Thermo Scientific EASY-IC ion source improves mass-to-charge (m/z) ratio assignment accuracy to under 1 ppm by enabling real-time adjustments of the instrument’s m/z calibration.

Improve m/z ratio assignment accuracy to under 1 ppm with the Easy-IC ion source, which incorporates a secondary ion source that delivers a regulated number of calibrant ions into the analyte ions. This feature enables real-time fine adjustment of the instrument's m/z calibration, correcting otherwise uncompensated errors due to temperature fluctuations and scan-to-scan variations.


With the option to extend the mass range up to m/z 8000, the Thermo Scientific BioPharma option enables you to realize complete characterization of intact proteins under both native or denaturing conditions. Isotopic resolution of sub-units and the highest quality spectral information facilitates your in-depth characterization needs from intact, top and middle-down through to peptide mapping, all with speed, mass accuracy and quality.

Proteome Discoverer software

Proteomics research requires more than just simple identification. Thermo Scientific Proteome Discoverer software offers a full suite of analysis tools with the flexibility to address multiple research workflows, and an easy-to-use, wizard-driven interface. Confidently interpret your data with sophisticated statistical and visualization tools unique to Proteome Discoverer software, and utilize third-party algorithms to take your analysis to the next level.

Recommended for the following workflows:

  • Iterative search strategies
  • Multi-search engine comparison
  • Glycopeptide identification and quantification
  • Phosphopeptide ID and quantification
  • Top-down proteomics
  • Cross-linking


BioPharma Finder software

Complete protein characterization can be challenging, whether you are performing intact protein mass analysis, top- and middle-down analysis, peptide mapping or multi-attribute method (MAM) workflows. Access workflows to facilitate comprehensive interpretation and data visualization, confidently characterizing your biologics with speed and ease. Thermo Scientific BioPharma Finder software helps you choose the right path for confident protein characterization.

Recommended for the following workflows:

  • Intact analysis
  • Top-down and middle-down analysis
  • Peptide mapping
  • Multi-attribute method (MAM)


Almanac web-based monitoring and management

Using the Thermo Scientific Almanac web- and smartphone-based application gives you the ability to remotely access and manage your LC-MS systems, freeing you to focus on other important tasks during your day.

Check real-time system status and acquisitions, set up automated e-mails to notify you of a completed acquisition or error, schedule instrument access, monitor utilization, or send service files to aid in system diagnostics and maximize up-time.

Key Almanac Tools

  • Instrument Connect Platform: manage your systems and data in a single location
  • Instrument Status: see what is happening in real time
  • Instrument Logbook and Events: easily see what has happened and log important information
  • Instrument Utilization Reports: evaluate system usage over time
  • Instrument Scheduler: manage system access and utilization
  • Push to Service: minimize time taken to diagnose issues
Monitor, Store and Share

Connect your laboratory: Always know what’s going on within your laboratory with any cloud-connected device. Maximize system utilization with long-term trending of system usage and sample throughput, through to checking data acquisition and multiple other productivity tools.

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Target applications for Orbitrap Exploris 480 Mass Spectrometer


Orbitrap Exploris 480 mass spectrometer ordering guides


LC-MS webinar series

LC-MS webinar series

Expand your application knowledge

Our LC-MS on-demand webinar series expands your application knowledge for pharma, biopharma, environmental, omics, food, forensics and clinical research and more. Learn how the latest high-performance mass spectrometry solutions can enable your laboratory to solve your most pressing analytical challenges, regardless of sample type. 


ASMS 2019 Posters