Fully-Characterized to Maximize Your Results

High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology, increase the ability to draw in vitro/in vivo correlations and make sound decisions regarding a compound's fate.  Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

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Each lot is tested for:

  • Phase I and II enzyme activities
  • Proper morphology
  • Viabilities generally >75%
  • Attachment efficiency
  • Monolayer formation and integrity

Learn more about the characterization of human hepatocytes

Prequalified and ready for your application

To facilitate your experiments, each lot of hepatocytes is qualified for particular applications:

  • Suspension hepatocytes - suitable for most short term metabolism studies
  • Plateable hepatocytes - useful for induction, plated metabolism, and transporter applications

Learn more about our applications testing and specifications

Gibco Human Spheroid-Qualified Hepatocytes

Our Spheroid qualified hepatocytes are the first off-the-shelf cryopreserved human cells for generation of hepatic spheroids in your own lab. Each vial contains a minimum of five million viable cells, sufficient for up to 16, 96-well plates of hepatic spheroids.

Induction Qualified Cryopreserved Human Hepatocytes

Each lot of cryopreserved human hepatocytes is inducible and suitable for use in experiments monitoring in vitro enzyme induction.  The potential of known clinically relevant enzyme inducers represented by 3-methylcholanthrene (3-MC, a prototypical CYP1A enzyme inducer, 3µM), phenobarbital (PB, a prototypical CYP2B inducer, 1000 µM) and rifampicin (RIF, a prototypical CYP3A enzyme inducer, 10 µM) is assessed to verify that each lot is inducible.

Transporter Qualified Cryopreserved Human Hepatocytes

Cryopreserved human hepatocytes for use in plated and suspension metabolism applications.  Each lot is assessed for the ability to express functional active uptake transport.  For suspension cryopreserved hepatocytes, the oil spin method is used.  Taurocholate, digoxin, and estradiol 17β glucuronide (E2-17G), substrates of the uptake and efflux transporters NTCP and BSEP, OATP1B3 and MDR1, OATPs and MRP2.

Metabolism Qualified Cryopreserved Human Hepatocytes

Cryopreserved human hepatocytes for use in plated and suspension metabolism applications. Prototypical cytochrome P450 substrates midazolam, tolbutamide, and dextromethorphan were used to assess the enzymatic function of CYP3A4⁄5, CYP2C9 and CYP2D6 respectively, for plated hepatocytes.  For suspension lots, each lot is characterized for CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4, CYP3A4/5, FMO activity. HEP10(TM) Pooled Lots are further characterized for NTCP, OATP1B3, and OATP transporter pathways.