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Berthold Technologies Mithras² LB 943 and Tristar² S LB 942 have been rigorously tested using either the Terbium Assay Application Note or the Europium Assay Application Note with demonstrated performance as shown in the setup guide.

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Before using LanthaScreen Terbium and Europium Assays

Test your plate reader setup

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen assay.

Assay technology Adapta and LanthaScreen Eu GeneBLAzer LanthaScreen Tb Predictor Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup guide Mithras2 LB 943 Microplate Reader Europium (Eu) Assay Setup Guide Compatible, not tested  Mithras2 LB 943 Microplate Reader Terbium (Tb) Assay Setup Guide

Compatible

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Tested
Excitation filter 340x26 405x10 320x40   405x10
Emission 1 filter 665xm7uv 460m25 520xm10uv   460m25
Emission 2 filter 620xm10uv 535m25 495xm10uv   535m25
Dichronic mirror Fixed Fixed Fixed   Fixed
Note: filters for the Mithras² LB 943 use the notation: excitation filters as center wavelength x bandwidth (nm) and emission filters as center wavelength xm bandwidth or center wavelength m bandwidth (nm). Contact Berthold Technologies for information on ordering filters for the microplate readers listed above.
Assay technology Adapta and LanthaScreen Eu GeneBLAzer LanthaScreen Tb Predictor Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup guide Tristar2 S LB 942 Microplate Reader Europium (Eu) Assay Setup Guide
Compatible, not tested Tristar2 S LB 942 Microplate Reader Terbium (Tb) Assay Setup Guide

Not compatible

Compatible, not tested 
Excitation filter 340x26 405/10 320x40   405/10
Emission 1 filter 665xm7uv 460/25 520xm10uv   460/25
Emission 2 filter 620xm10uv 535/25 495xm10uv   535/25
Dichronic mirror Fixed Fixed Fixed   Fixed
Excitation and Emission filters for the Tristar² S LB 942 use the notation: center wavelength/bandwidth (nm). Contact Berthold Technologies for information on ordering filters for the microplate readers listed above.