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Berthold Technologies Mithras² LB 943 and Tristar² S LB 942 have been rigorously tested using either the Terbium Assay Application Note or the Europium Assay Application Note with demonstrated performance as shown in the setup guide.

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Before using LanthaScreen Terbium and Europium Assays

Test your plate reader setup

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen assay.

Assay technologyAdapta and LanthaScreen EuGeneBLAzerLanthaScreen TbPredictorZ'-LYTE
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup guideMithras2 LB 943 Microplate Reader Europium (Eu) Assay Setup GuideCompatible, not tested Mithras2 LB 943 Microplate Reader Terbium (Tb) Assay Setup Guide

Compatible

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Tested
Excitation filter340x26405x10320x40 405x10
Emission 1 filter665xm7uv460m25520xm10uv 460m25
Emission 2 filter620xm10uv535m25495xm10uv 535m25
Dichronic mirrorFixedFixedFixed Fixed
Note: filters for the Mithras² LB 943 use the notation: excitation filters as center wavelength x bandwidth (nm) and emission filters as center wavelength xm bandwidth or center wavelength m bandwidth (nm). Contact Berthold Technologies for information on ordering filters for the microplate readers listed above.
Assay technologyAdapta and LanthaScreen EuGeneBLAzerLanthaScreen TbPredictorZ'-LYTE
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup guideTristar2 S LB 942 Microplate Reader Europium (Eu) Assay Setup Guide
Compatible, not testedTristar2 S LB 942 Microplate Reader Terbium (Tb) Assay Setup Guide

Not compatible

Compatible, not tested 
Excitation filter340x26405/10320x40 405/10
Emission 1 filter665xm7uv460/25520xm10uv 460/25
Emission 2 filter620xm10uv535/25495xm10uv 535/25
Dichronic mirrorFixedFixedFixed Fixed
Excitation and Emission filters for the Tristar² S LB 942 use the notation: center wavelength/bandwidth (nm). Contact Berthold Technologies for information on ordering filters for the microplate readers listed above.
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