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The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance.

Before using LanthaScreen® Terbium and Europium assays

Test your plate reader set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.

Download Terbium assay application note

Download Europium assay application note

SpectraMax® Paradigm® Microplate Detection Platform

 

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide
Application Note
Poster--
Excitation Filter 406/15 532/25 
Emission 1 Filter 465/35 595/35  
Emission 2 Filter 535/25 595/35  
Dichroic Mirror 508   
Optic Module GeneBLAzer® FP rhodamine  

 

SpectraMax® M5/M5e Microplate Reader
& FlexStation® 3 Benchtop Multi-Mode Microplate Reader

 

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide

Reader not
compatible

Application Note-----
Poster-----
Excitation332/12410/12332/12 410/12
Emission 1620/12458/12488/12 458/12
Emission 2666/12522/12518/12 522/12
Dichroic Mirror590/630435/495420 435/495

FilterMax™ F5 Multi-Mode Microplate Reader

 

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup GuideReader not compatibleReader not compatibleReader not compatible
Application Note
Poster--
Excitation Filter 405/30   405/30
Emission 1 Filter 465/35   465/35
Emission 2 Filter 535/25   535/25
Dichroic Mirror     

 

Analyst® Multimode Reader


   

 

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide

-

-

 

-

-

Application Note-----
Poster-----
Excitation Filter (25 mm)340/30 (PV00225)405/20340/30 (PV00225)530/25405/20
Emission 1 Filter (25 mm)620/7.5460/40495/10 (PV00325)580/10460/40
Emission 2 Filter (25 mm)665/7.5530/25520/25 (PV00325)580/10530/25
Dichroic Mirror380 or
400
425 or
50/50
380 or
400
561425 or
50/50

 

* Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above.

** Some compatible filter sets are available for purchase from Life Technologies.  Click on selected filter sets for more information.

Analyst®, SpectraMax® and FlexStation® are registered trademarks of Molecular Devices.

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