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The PHERAstar, PHERAstar Plus, Omega, and CLARIOstar microplate readers have received our LanthaScreen Certified Plus designation which ensures that these readers are validated to strict standards in instrument setup and assay performance.

Before using LanthaScreen Terbium and Europium assays

Test your plate reader set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen assay.

Download Terbium assay application note
Download Europium assay application note


PHERAstar FSX Microplate Reader

Instrument configuration

Assay technologyAdapta &
LanthaScreen
Europium
GeneBLAzerLanthaScreen
Terbium
PredictorZ′-LYTE
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup guideCompatible but not testedCompatible but not tested
Compatible but not tested
Application note
Poster
Excitation filterEX TR400–10EX TR532/25400–10
Emission 1 filter665–10450–10520–10595/35450–10
Emission 2 filter620–10520–10490–10NA520–10
Dichroic mirrorLP TRLP 426
LP TRLP 565LP426

CLARIOstar/CLARIOstarPlus Microplate Readers

Instrument configuration

Assay technologyAdapta &
LanthaScreen
Europium
GeneBLAzerLanthaScreen
Terbium
PredictorZ'LYTE
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup guideCompatible but not tested
Application note
Poster
Excitation filterEX TR400-10EX TR540-20400-10
Emission 1 filter665-10450-10520-10590-20450-10
Emission 2 filter620-10520-10490-10NA520-10
Dichroic mirrorLP TRLP 426LP TRLP 565LP 426

*LVF monochromator setting
+Linear variable dichroic setting


PHERAstar FS Microplate Reader

Instrument configuration

Assay technologyAdapta &
LanthaScreen
Europium
GeneBLAzerLanthaScreen
Terbium
PredictorZ′-LYTE
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup guide 
Application noteView
Poster
Excitation filter337405337532/25405
Emission 1 filter665460520595/35460
Emission 2 filter620530490595/35530
Dichroic mirrorIncludedIncludedIncludedIncludedIncluded
Optic moduleHTRF®FI 405 530 460LanthaScreenFP rhodamineFI 405 530 460

PHERAstar/PHERAstar Plus Microplate Readers

Instrument configuration

Assay technologyAdapta &
LanthaScreen Europium
GeneBLAzerLanthaScreen TerbiumPredictorZ′-LYTE
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup guidePHERAstar Plus compatible not tested 
Application note
Poster
Excitation filter337337540400
Emission 1 filter665520590440
Emission 2 filter620490590520 
Dichroic mirrorIncludedIncludedIncludedIncluded
Optic moduleHTRF®LanthaScreenRed FPNA

FLUOstar Omega & POLARstar Omega Microplate Readers

Instrument configuration

Assay technologyAdapta &
LanthaScreen
Europium
GeneBLAzerLanthaScreen
Terbium
PredictorZ′-LYTE
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup guide! POLARstar not compatible
Application note
Poster
Excitation filter337400337NA400
Emission 1 filter665440520NA440
Emission 2 filter620520490NA520


Note: for the Adapta assay and LanthaScreen Europium and Terbium assays both the FLUOstar Omega and POLARstar Omega need BMG LABTECH's TRF head accessory

! The FLUOstar Omega is not equipped to perform the Predictor assay and the POLARstar Omega is not compatible


FLUOstar OPTIMA & POLARstar OPTIMA Microplate Readers

Instrument configuration

Assay technologyAdapta &
LanthaScreen
Europium
GeneBLAzerLanthaScreen
Terbium
PredictorZ′-LYTE
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup guideReaders not compatible Readers not compatible! POLARstar not compatible
Application note
Poster
Excitation filter400NA400
Emission 1 filter440NA440
Emission 2 filter520NA520 


! The FLUOstar OPTIMA is not equipped to perform the Predictor assay and the POLARstar OPTIMA is not compatible

* Filters shown as center wavelength (nm); Contact BMG LABTECH for information on ordering optics modules & filters suitable with the microplate readers listed above.

HTRF® is a registered trademark of Cisbio Assays

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