The PHERAstar, PHERAstar Plus, Omega, and CLARIOstar microplate readers have received our LanthaScreen Certified Plus designation which ensures that these readers are validated to strict standards in instrument setup and assay performance.

Before using LanthaScreen Terbium and Europium assays

Test your plate reader set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen assay.

Download Terbium assay application note
Download Europium assay application note


PHERAstar FSX Microplate Reader

Instrument configuration

Assay technology Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor Z′-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup guide Compatible but not tested Compatible but not tested
Compatible but not tested
Application note
Poster
Excitation filter EX TR 400–10 EX TR 532/25 400–10
Emission 1 filter 665–10 450–10 520–10 595/35 450–10
Emission 2 filter 620–10 520–10 490–10 NA 520–10
Dichroic mirror LP TR LP 426
LP TR LP 565 LP426

CLARIOstar Microplate Readers

Instrument configuration

Assay technology Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor Z'LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup guide Compatible but not tested
Application note
Application note
Poster
Excitation filter EX TR 400-10 EX TR 540-20 400-10
Emission 1 filter 665-10 450-10 520-10 590-20 450-10
Emission 2 filter 620-10 520-10 490-10 NA 520-10
Dichroic mirror LP TR LP 426 LP TR LP 565 LP 426

*LVF monochromator setting
+Linear variable dichroic setting


PHERAstar FS Microplate Reader

Instrument configuration

Assay technology Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor Z′-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup guide  
Application note View
Poster
Excitation filter 337 405 337 532/25 405
Emission 1 filter 665 460 520 595/35 460
Emission 2 filter 620 530 490 595/35 530
Dichroic mirror Included Included Included Included Included
Optic module HTRF® FI 405 530 460 LanthaScreen FP rhodamine FI 405 530 460

PHERAstar/PHERAstar Plus Microplate Readers

Instrument configuration

Assay technology Adapta &
LanthaScreen Europium
GeneBLAzer LanthaScreen Terbium Predictor Z′-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup guide Reader not
compatible
 
Application note View
Poster View
Excitation filter 337 337 540 400
Emission 1 filter 665 520 590 440
Emission 2 filter 620 490 590 520 
Dichroic mirror Included Included Included Included
Optic module HTRF® LanthaScreen Red FP NA

FLUOstar Omega & POLARstar Omega Microplate Readers

Instrument configuration

Assay technology Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor Z′-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup guide ! POLARstar not compatible
Application note
Poster
Excitation filter 337 400 337 NA 400
Emission 1 filter 665 440 520 NA 440
Emission 2 filter 620 520 490 NA 520


Note: for the Adapta assay and LanthaScreen Europium and Terbium assays both the FLUOstar Omega and POLARstar Omega need BMG LABTECH's TRF head accessory

! The FLUOstar Omega is not equipped to perform the Predictor assay and the POLARstar Omega is not compatible


FLUOstar OPTIMA & POLARstar OPTIMA Microplate Readers

Instrument configuration

Assay technology Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor Z′-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup guide Readers not compatible   Readers not compatible ! POLARstar not compatible
Application note
Poster
Excitation filter 400 NA 400
Emission 1 filter 440 NA 440
Emission 2 filter 520 NA 520 


! The FLUOstar OPTIMA is not equipped to perform the Predictor assay and the POLARstar OPTIMA is not compatible

* Filters shown as center wavelength (nm); Contact BMG LABTECH for information on ordering optics modules & filters suitable with the microplate readers listed above.

HTRF® is a registered trademark of Cisbio Assays