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The PHERAstar, PHERAstar Plus, Omega, and CLARIOstar® microplate readers have received our LanthaScreen® Certified Plus designation which ensures that these readers are validated to strict standards in instrument setup and assay performance.

Before using LanthaScreen® Terbium and Europium assays

Test your plate reader set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.

Download Terbium assay application note

Download Europium assay application note

CLARIOstar® Microplate Readers

 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Predictor™ Z'LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Compatible but not tested

Application Note -

-

- App Note -
Poster - - - - -
Excitation Filter EX TR 400-10 EX TR 540-20 400-10
Emission 1 Filter 665-10 450-10 520-10 590-20 450-10
Emission 2 Filter 620-10 520-10 490-10 NA 520-10
Dichroic Mirror LP TR LP 426 LP TR LP 565 LP 426

 

*LVF monochromator setting
+Linear variable dichroic setting

 

PHERAstar FS Microplate Reader

 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

 

Application Note -

View

- - -
Poster - - - - -
Excitation Filter 337 405 337 532/25 405
Emission 1 Filter 665 460 520 595/35 460
Emission 2 Filter 620 530 490 595/35 530
Dichroic Mirror Included Included Included Included Included
Optic Module HTRF® FI 405 530 460 LanthaScreen® FP rhodamine FI 405 530 460

 

PHERAstar/PHERAstar Plus Microplate Readers

   

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Reader not
compatible

 

Application Note - - - View -
Poster - - - View -
Excitation Filter 337 - 337 540 400
Emission 1 Filter 665 - 520 590 440
Emission 2 Filter 620 - 490 590 520 
Dichroic Mirror Included - Included Included Included
Optic Module HTRF® - LanthaScreen® Red FP NA

 

FLUOstar Omega & POLARstar Omega Microplate Readers

   

 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

! POLARstar not compatible

Application Note - - - - -
Poster - - - - -
Excitation Filter 337 400 337 NA 400
Emission 1 Filter 665 440 520 NA 440
Emission 2 Filter 620 520 490 NA 520


Note: for the Adapta® assay and LanthaScreen® Europium and Terbium assays both the FLUOstar Omega and POLARstar Omega need BMG LABTECH's TRF head accessory

! The FLUOstar Omega is not equipped to perform the Predictor™ assay and the POLARstar Omega is not compatible

 

FLUOstar OPTIMA & POLARstar OPTIMA Microplate Readers

   

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Readers not compatible

 

Readers not compatible

! POLARstar not compatible

Application Note - - - - -
Poster - - - - -
Excitation Filter - 400 - NA 400
Emission 1 Filter - 440 - NA 440
Emission 2 Filter - 520 - NA 520 


! The FLUOstar OPTIMA is not equipped to perform the Predictor™ assay and the POLARstar OPTIMA is not compatible



* Filters shown as center wavelength (nm); Contact BMG LABTECH for information on ordering optics modules & filters suitable with the microplate readers listed above.

HTRF® is a registered trademark of Cisbio Assays