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BioTek’s Synergy™ H4 and Synergy™ 2 multi-mode microplate readers have received the LanthaScreen® Certified Plus designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance.

Before Using LanthaScreen® Terbium and Europium Assays

Test Your Plate Reader Set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.

Download Terbium assay application note

Download Europium assay application note

Synergy™ H4 Hybrid Multi-Mode Microplate Reader

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide

Application NoteViewViewViewViewView
Poster-ViewViewViewView
Excitation Filter (18 mm) 340/30400/30340/30530/25 400/30 
Emission 1 Filter (18 mm) 620/10460/40495/10590/35460/40 
Emission 2 Filter (18 mm) 665/8528/20520/25590/35 528/20 
Dichroic Mirror 400NA400 570435 

 

Synergy™ 2 Multi-Mode Microplate Reader

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Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide

Application NoteViewViewViewViewView
Poster-ViewViewViewView
Excitation Filter (18 mm)340/30400/30340/30530/25 400/30 
Emission 1 Filter (18 mm)620/10460/40495/10590/35460/40 
Emission 2 Filter (18 mm)665/8528/20520/25590/35 528/20 
Dichroic Mirror400NA400570435

Synergy™ HT Multi-Mode Microplate Reader

 

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide

Reader not compatible

Reader not tested

Reader not compatible

Reader not compatible

Reader not tested

Application Note-View--View
Poster-----
Excitation Filter (18 mm)-400/30--400/30 
Emission 1 Filter (18 mm)-460/40--460/40 
Emission 2 Filter (18 mm)-528/20--528/20 

FLx800™ Fluorescence Microplate Reader

 

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide

Reader not compatible

Reader not compatible

Reader not compatible

Application Note-----
Poster-View--View
Excitation Filter (18 mm)-400/30--400/30 
Emission 1 Filter (18 mm)-460/40--460/40 
Emission 2 Filter (18 mm)-528/20--528/20 



* Filters (diameter) shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm). Contact BioTek Instruments for information on ordering filters and dichroic mirrors suitable with the microplate readers listed above. 

Synergy™ and FLx800™ are trademarks of BioTek Instruments.

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