A powerful innovation in protein quantitation
Invitrogen ProQuantum immunoassays are designed for easy-to-run, high-performance, target-specific protein detection, with no proprietary instrument to purchase. Utilizing proximity-based amplification technology, ProQuantum assats offer the analyte specificity of antibody–antigen binding with the signal detection and amplification capabilities of qPCR to achieve a highly sensitive assay. ProQuantum assays can also typically detect lower levels of protein with lower sample consumption than traditional methods.
Watch the on-demand webinar to get the technical details of the assay from an R&D expert.
Download the product brochure or watch the instructional video
To learn more about the technology and benefits of ProQuantum high-sensitivity immunoassays, how easy it is to run an assay and how they can help you in your research, download the ProQuantum high-sensitivity immunoassays brochure or watch the instructional video by clicking the appropriate button below:
Ordering ProQuantum immunoassays
NEW Protein targets
- Human GRO-α
- Human IFN-β
- Human IL-21
- Human IL-27
- Human TNF-β
- Mouse IL-6
- Mouse TNF-α
Available protein targets include:
ProQuantum immunoassays benefits at-a-glance:
- High-sensitivity—detect low levels of proteins with greater sensitivity than traditional methods (Figure 1)
- Broad dynamic range—≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
- Small sample consumption—use 2–5 μL of sample (instead of 75 μL for triplicate wells with other methods)
- Fast, easy workflow—typically 2 hours from sample to answer, no wash steps, and a single 1-hour incubation
- No proprietary instrument to purchase—runs on any real-time PCR instrument
- Includes intuitive cloud-based software—comprehensive data analysis and statistical group–wise comparison
ProQuantum immunoassays utilize a matched pair of target-specific antibodies each conjugated to a DNA oligonucleotide. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology. During antibody–analyte binding, the two DNA oligos are brought into close proximity, which then allows for ligation of the two strands and subsequent creation of template strand for amplification (Figure 2).
Figure 1. Standard curve for Invitrogen ProQuantum High-Sensitivity Human IFNγ, IL-1β, IL-8, and IL-6 immunoassay kits (red) shows larger dynamic range and improved separation at the low end of the curve for greater sensitivity than normal and ultrasensitive ELISAs (blue).
Thermo Fisher Scientific is committed to designing our products with the environment in mind—it’s part of how we enable our customers to make the world healthier, cleaner, and safer. This fact sheet provides the rationale behind the environmental claim that Invitrogen ProQuantum Immunoassay Kits generate less waste and have more sustainable packaging than traditional ELISA kits. To learn more download the green features fact sheet
Connect with us
Request the next target release
We are continuously developing new ProQuantum immunoassay kits. You can contribute to our product development roadmap. Send us an email to let us know what protein targets you would like to see next.
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We are continuously releasing new ProQuantum immunoassay kits. Stay in the loop and sign up to receive our new product release announcements.
For Research Use Only. Not for use in diagnostic procedures.