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Media Development
Optimized media development solutions and multi-omics capabilities
Optimize your unique medium and process for increased titers, quality and manufacturability by partnering with Gibco PD-Express Services. We have been assisting clients with their cell culture needs for more than 50 years, successfully completing more than 100 medium development projects. These include utilizing various mammalian and insect cell lines for the production of proteins and viruses. Our flexible media development options include our new multi-omics workflow—allowing you to characterize your process to improve productivity.
Multi-omics media development workflow
Click image to enlarge
What is the omics difference?
The Gibco PD-Express Services multi-omics workflow combines spent media and cellular component analyses of up to 8,000 molecules—including 7,000 proteins and 1,000 metabolites.
This service relies on best-in-class technology to support data intelligence. This includes the highest resolution UPLC-MS instrumentation and the use of predictive modeling and bioinformatics.
Potential benefits of a multi-omics approach
- Increased cell line productivity/titer
- Enhanced protein quality
- Improved process and cost efficiencies
Flexible collaboration choices
Our multi-omics media development service provides you the option to work with our experts as an extension of your own team in two different ways.
| Consultation service | Full service project |
|---|---|---|
Cell culture performed by customer | X | |
Spent media and multi-omics analyses performed by Thermo Fisher Scientific | X | |
Workflow and process development support | X | X |
Dedicated project team | X | X |
Industry-leading technology
Our multi-omics workflow relies on best-in-class technology to support our data intelligence. This includes:
- Highest resolution UPLC-MS instrumentation
- Predictive analytics
- Bioinformatics
Traditional media development workflow
Our traditional media development workflow uses the classical approach to media optimization. Spent media analyses of fewer than 50 molecules, including select water soluble vitamins and amino acids, are used in iterative DOE (design of experiment) steps to optimize medium and feed formulations to help you meet your unique process goals.
- Customized solutions can be completed at your facility or ours
- Media is developed with manufacturing in mind–ensuring formulations are scalable, able to be manufactured in multiple formats, and aligned with your cost expectations
- When speed is important, our Quick Start process is an option for traditional media development workflow customers. The program allows projects to kick off faster with less legal paperwork getting you to results sooner
You can flexibly choose to work with the PD-Express Services team at your site or at a Gibco site through a consultation service or full-service project, respectively.
Multi-omics media development workflow
Click image to enlarge
What is the omics difference?
The Gibco PD-Express Services multi-omics workflow combines spent media and cellular component analyses of up to 8,000 molecules—including 7,000 proteins and 1,000 metabolites.
This service relies on best-in-class technology to support data intelligence. This includes the highest resolution UPLC-MS instrumentation and the use of predictive modeling and bioinformatics.
Potential benefits of a multi-omics approach
- Increased cell line productivity/titer
- Enhanced protein quality
- Improved process and cost efficiencies
Flexible collaboration choices
Our multi-omics media development service provides you the option to work with our experts as an extension of your own team in two different ways.
| Consultation service | Full service project |
|---|---|---|
Cell culture performed by customer | X | |
Spent media and multi-omics analyses performed by Thermo Fisher Scientific | X | |
Workflow and process development support | X | X |
Dedicated project team | X | X |
Industry-leading technology
Our multi-omics workflow relies on best-in-class technology to support our data intelligence. This includes:
- Highest resolution UPLC-MS instrumentation
- Predictive analytics
- Bioinformatics
Traditional media development workflow
Our traditional media development workflow uses the classical approach to media optimization. Spent media analyses of fewer than 50 molecules, including select water soluble vitamins and amino acids, are used in iterative DOE (design of experiment) steps to optimize medium and feed formulations to help you meet your unique process goals.
- Customized solutions can be completed at your facility or ours
- Media is developed with manufacturing in mind–ensuring formulations are scalable, able to be manufactured in multiple formats, and aligned with your cost expectations
- When speed is important, our Quick Start process is an option for traditional media development workflow customers. The program allows projects to kick off faster with less legal paperwork getting you to results sooner
You can flexibly choose to work with the PD-Express Services team at your site or at a Gibco site through a consultation service or full-service project, respectively.
Benefits of a media development collaboration
Improve process performance
Whether you are having trouble producing a complex protein, achieving the required titer to meet market demands, or achieving a specific glycoform profile, Gibco PD-Express Services can develop a custom solution for you.
Example of potential project goals:
- Manufacture challenging molecules (fusion proteins, enzymes, viruses)
- Increase product titers
- Modify product quality attributes (e.g., achieve specific glycoform profile)
Improve manufacturability of the medium
We apply our knowledge of media design, raw material sourcing, and manufacturing to improve process consistency, reduce risk of adventitious agents, and meet quality and regulatory requirements. Over 98% of our medium development projects have resulted in a chemically defined medium solution.
Examples of improvements gained in project:
- Eliminate serum from the medium
- Eliminate or reduce other components of animal origin (e.g., insulin, transferrin, cholesterol)
- Eliminate or reduce other complex components (e.g., recombinant proteins, hydrolysates)
- Replace components that do not have a robust source/supply chain
- Replace reagent grade, non-compendial components with those of appropriate grade for the target market (e.g., EP, BP, USP, JP)
- Reformat liquid medium as a dry powder or Advance Granulation Technology (AGT)
Establish a platform medium
A medium and feed platform is helpful to customers who are utilizing multiple clones to produce different proteins. Being able to use the same medium and feed for all processes streamlines the development, scale-up, and tech transfer activities to allow customers to move quickly from discovery to commercial launch.
Example of potential project goals:
- Develop a medium and feed to support different cell lines from a single host cell line
- Develop a universal medium and feed to work with different cell types
- Develop a feed solution for a specific cell line to work with a platform medium
Specific application for media development projects
Additionally, Gibco PD-Express Services has the capability to leverage their medium development expertise to specific applications and needs in the industry. Some examples include:
- T-cell processes
- Viral vaccine processes
- Biosimilars processes
- Cell-based assays
Dedicated project management
Both our traditional and our multi-omics workflows employ a phased approach to our projects with a go/no go decision point at the end of each phase. In addition, a Certified Project Manager will be assigned to your project to act as a single dedicated point of contact for all of your needs, and to drive on time completion.
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