Identify an optimal formulation for your target cell line

Manufacturers of adeno-associated viral (AAV) vectors need to scale up quickly and cost-effectively to meet the increasing demand from the gene therapy market. But testing and optimizing multiple catalog media takes time and expertise. What if you had a panel of media and an expert in media optimization to help you?

The Gibco Viral Vector HEK Media Panel provides a diverse offering of media to accelerate gene therapy development and production. The media panel can be used for a broad range of HEK293 cells. It is not dependent on specific transfection reagents or on specific techniques.  As a result, the ready-to-use format can help identify a formulation for your target cell line that is optimized for higher titers, is scalable, and may be further customized to improve performance.

Throughout the evaluation process our scientists will provide technical support. Acting as an extension of your R&D team, our scientists will provide process consultation, data analysis, and a summary report to expedite media optimization and cGMP manufacturing.

Viral Vector HEK Media Panel screen of one HEK cell line

Responses to the media panel vary based on HEK cell line origin and adaptation strategy performed. A HEK293 cell line grown in a chemically defined medium was adapted to the five media formulations in the Viral Vector HEK Media Panel and FreeStyle F17 Expression Medium. AAV2 titers were generated using triple transfection with a polymer-based reagent (biological triplicate performed). No titer enhancer was used to assess the effect of base media compared to the control titer obtained in FreeStyle F17 Expression Medium as indicated by the blue line (x=1).

Podcast: Inside Bioprocessing Manufacturing of Gene Therapies

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