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Nozzles, consumables, spectral software, and reagents for the Bigfoot Spectral Cell Sorter
Accessories for continued operation of the Bigfoot Spectral Cell Sorter include interchangeable nozzles, QC beads, and sheath fluid. Choose among a wide selection of fluorophores and functional dyes to identify, sort, and analyze cell populations. Add a Sasquatch software upgrade to support advanced analysis for spectral flow cytometry. Spectral unmixing capability is not available for all configurations. Please review options with your sales representative.
Nozzles
With the Bigfoot Spectral Cell Sorter, you can choose from 70-, 100-, 120-, and 150-µm ceramic nozzle tips with adjustable pressure settings. A swap tip wizard simplifies setup of the nozzle tips.
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Consumables
Bigfoot QC Beads, stored in their own vial in the multi-sample loader, are essential for daily QC of the Bigfoot Spectral Cell Sorter, while Bigfoot Sheath Fluid is used to focus the hydrodynamic stream.
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Reagents
Thermo Fisher Scientific offers many reagents suitable for flow cytometry across several families of products. Choose from a broad selection of fluorophores that are conjugated to your marker of choice, or a range of functional dyes illuminating viability, DNA content, apoptosis, cell cycle, proliferation, and more.
We offer a full range of Brilliant Ultra Violet™ and Brilliant Violet™ Polymer Dyes for superior performance in high-parameter panels. With our polymer dyes, you are completely covered for UV and Violet channel needs.
Our line of NovaFluor dyes is specifically designed for higher resolution in high-parameter immunophenotyping panels, with narrow excitation ranges for minimal cross-laser excitation. They can lessen the need for compensation in conventional experiments and can reduce panel complexity in spectral flow cytometry experiments.
Selected available fluorophores based on usage, benefits, and intended applications
| Family |
Type |
Benefit |
Invitrogen fluorophore |
|---|---|---|---|
| Organic dyes— small, stable molecules |
Original |
• FITC is cost-efficient |
FITC |
| Pacific dyes |
• Some of the dimmest dyes | Pacific Blue Pacific Orange |
|
| Alexa Fluor dyes |
• Photostable dyes that range across the visible spectrum
• Used in flow cytometry and imaging • Named for their excitation wavelengths |
Alexa Fluor 405 Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 561 Alexa Fluor 647 Alexa Fluor 660 Alexa Fluor 700 |
|
| eFluor organic dyes | • Engineered for detection for flow cytometry • Named for their emission wavelength |
eFluor 450 eFluor 506 eFluor 660 |
|
| Large, protein‐based molecules |
Original |
• Cost-efficient • Some of the brightest dyes available |
APC PE PerCP |
| Tandem dyes | • Dyes occupy different channels from the donor molecule, and this can be used to build larger panels | APC-Cyanine5 APC-Cyanine7 PE-Cyanine5 (TRI-COLOR) PE-Cyanine5.5 PE-Cyanine7 PE–Texas Red PerCP-Cyanine5.5 PE–Alexa Fluor 610 PE–Alexa Fluor 700 APC–Alexa Fluor 750 PE–eFluor 610 PerCP–eFluor 710 APC–eFluor 780 |
|
| Polymer dyes | Super Bright dyes and their tandems | • Excited by the 405 nm violet laser • Minimal spillover into other channels • Add Super Bright Complete Staining Buffer (Cat. No. SB-4401-42) when using two or more polymer dyes to lower background levels |
Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 702 Super Bright 780 |
| Polymer dyes— recent dye innovation |
Invitrogen eBioscience Brilliant Violet™ and Brilliant Ultra Violet™ dyes | • Excited by the violet and ultra violet lasers • Invitrogen Brilliant Stain Buffer (Cat. No. 00-4409-75) when using two or more polymer dyes to lower background levels |
Brilliant Ultra Violet 395 Brilliant Ultra Violet 496 Brilliant Ultra Violet 563 Brilliant Ultra Violet 615 Brilliant Ultra Violet 661 Brilliant Ultra Violet 737 Brilliant Ultra Violet 805 Brilliant Violet 421 Brilliant Violet 480 Brilliant Violet 650 |
| Quantum dots |
• Narrow emission • Large Stokes shift |
Qdot 605 Qdot 655 Qdot 705 Qdot 800 |
|
| NovaFluor dyes | DNA nanostructure-based dyes |
• Unique spectral signatures • Variable brightness • Low cross-laser excitation • Named for the exciting laser and emission spectrum |
NovaFluor Blue 510 NovaFluor Blue 530 NovaFluor Blue 555 NovaFluor Blue 585 NovaFluor Blue 610-30S NovaFluor Blue 610-70S NovaFluor Blue 660-40S NovaFluor Blue 660-120S NovaFluor Yellow 570 NovaFluor Yellow 590 NovaFluor Yellow 610 NovaFluor Yellow 660 NovaFluor Yellow 690 NovaFluor Yellow 700 NovaFluor Yellow 730 NovaFluor Red 660 NovaFluor Red 685 NovaFluor Red 700 NovaFluor Red 710 |
For detailed information on selecting fluorophores, assays, and reagents for spectral flow cytometry, including a featured webinar, a reagent selection guide, and an interactive spread matrix for selected lasers, see our article on Spectral Flow Cytometry Assays and Reagents.
Spectral software upgrade
Sasquatch Software (SQS) comes standard with all Bigfoot Spectral Cell Cytometers.
In addition, Spectral Sorting and Analysis for SQS is an optional software upgrade for spectral flow cytometry. The module provides spectral sorting capabilities using up to 55 fluorescence detectors for resolution of 30 or more colors.
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Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.