Performance data shows that the Bigfoot Spectral Cell Sorter resolves fluorescent signals accurately and precisely. Application data demonstrates the level of gating resolution and sort purity needed for studies of immunophenotyping and marker expression.
For additional application data, download the application notes listed in this section and on the Resources page.
Spherotech™ 8-peak beads run in key channels on the Bigfoot Spectral Cell Sorter show narrow, well-defined peaks.
Using the gated sort regions like the Treg gate above, six populations were sorted: CD8+ and cytotoxic T cells, B cells, NK cells, monocytes, and Tregs. For post-sort verification, each collection tube was reanalyzed for purity. Analysis of the sort populations (four of which are shown in the figure) demonstrated that the spectral unmixing capability of the Bigfoot Spectral Cell Sorter produced results with high efficiency and more than 98% purity. This sorting performance is equivalent to sorters using traditional compensation, allowing users to seamlessly transition between the two methods as experimental complexity increases.
Although polarization is a property of laser light that is often ignored in flow cytometry, it can be useful in differentiating certain cell types. For example, eosinophil granulocytes in human blood show relatively higher levels of depolarized laser light in a side scatter (SSC) detector due to their birefringent properties. Analysis of polarization has also been found useful in malaria diagnosis and marine phytoplankton studies.
All models of the Bigfoot Cell Sorter include additional FSC (front scatter) and SSC detectors for depolarized light detection. The figure shows the use of the SSC depolarized detector to identify eosinophils in lysed human whole blood. This differentiation does not require staining, leaving cells undisturbed and saving money on fluorophores. By adding a stain for CD16, other cell populations can be identified as well.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.