Choose your configuration up to 9 lasers and 60 channels

Configurations offering both spectral analysis and conventional compensation range from 5 to 9 lasers and up to 60 parameters, while configurations offering only traditional compensation come with 4 or 5 lasers and up to 35 parameters. Other than lasers and detectors, all configurations share the same specifications.


Configurations

All configurations of the Bigfoot Spectral Cell Sorter offer two or more forward and side scatter channels in addition to the fluorescence channels shown in the tables.

Configurations offering both spectral analysis and conventional compensation

Lasers

Number of fluorescence detection channels for included lasers

Total detection channels

Cat. No.

UV
349 nm

Violet
405 nm

Blue violet
445 nm

Blue
488 nm

Green
532 nm

Yellow green
561 nm

Red
594 nm

Far red
640 nm

Near-
IR
785 nm

5

12

12

 

7

 

12

 

5

 

53

PL00302

6

12

12

 

7

 

12

 

5

3

56

PL00301

6

12

12

4

7

 

12

 

5

 

57

PL00300

7

12

12

4

7

 

12

 

5

3

60

PL00299

9

12

12

7

12

4

4

3

60

PL00285

Configurations offering only conventional compensation

Lasers

Number of fluorescence detection channels for included lasers

Total detection channels

Cat. No.

UV
349 nm

Violet
405 nm

Blue violet
445 nm

Blue
488 nm

Green
532 nm

Yellow green
561 nm

Red
594 nm

Far red
640 nm

Near-
IR
785 nm

4

7

 

7

 

7

 

4

 

27

PL00304

5

7

7

 

5

 

7

 

4

35

PL00303


Specifications

Optics

Excitation lasers 349 nm, 405 nm, 445 nm, 488 nm, 532 nm, 561 nm,  594 nm, 640 nm, 785 nm
Optical power Free space delivery of 349 nm (100 mW), 405 nm (100 mW), 445nm (200 mW), 488 nm (125 mW), 532 nm (100 mW), 561 nm (100 mW), 594 nm (100 mW), 640 nm (100 mW), 785 nm (100 mW)
Beam alignment Fixed, 7 spatially separated pinholes
Detection parameters up to 55 fluorescence + 5 scatter
Scatter parameters Standard FSC and SSC 488 nm; small particle FSC 405 nm; depolarized FSC and SSC 488nm
Scatter resolution <0.2 µm scatter resolution from background with small particle detector
Pulse measurement Simultaneous measured peak, area, and width for sample input and output of every channel
Fluorescence sensitivity <100 MESF for FITC, PE, APC

Fluidics

Nozzles 70, 100, 120, and 150 µm ceramic nozzle tips with adjustable pressure settings
Sample input tube types 1.5 mL, 5 mL, and 15 mL
Temperature control 4°C-37°C for input and output of all media types
Agitation Automatic based on input tube type

Sorting

Event rate >100,000 eps acquisition with all 60 parameters
Sorting speed >70,000 eps with all parameters enabled
Sort devices Up to 6-way sorting into tubes; configurable tube holders include 1.5 mL, 5 mL, 15 mL, and 50 mL adapters; multi-way micro-well plate sorting up to 1,536 wells.

Biosafety

Compliance testing The Bigfoot Spectral Cell Sorter biosafety enclosure provides personnel and product protection similar to a Class II BSC. Test procedures and criteria laid out within NSF49 and EN12469 standards can be utilized to demonstrate performance.

Other

Dimensions 99 cm W x 99 cm D x 177 cm H (39 in W x 39 in D x 69.7 in H)
Fluidics cart None
Weight 400 kg
Monitor 32-inch, 4k
Power supply 100-240VAC 50/60Hz
Power consumption 750W max (instrument, PC and monitor)
Relative humidity 20-60% RH

For Research Use Only. Not for use in diagnostic procedures.