Thermo Scientific KingFisher instruments use magnetic bead technology to automate nucleic acid and protein purification. Our automated solution reduces user variability and hands-on time for a variety of applications and throughput levels, delivering a versatile system that can adapt to your changing research needs while maintaining consistent results.
Find the right KingFisher instrument for you
Size
Compact benchtop
Throughput
Low–Med throughput
6/12 samples per run
Volume range
30-5000 µl
Size
Benchtop
Throughput
Med–High throughput
24/96 samples per run
Volume range
20-5000 µl
Size
Integrate with liquid handler handler
Throughput
High throughput
96 samples per run
Volume range
50-5000 µl
Optimize your KingFisher instruments with kits & reagents, protocols, and plastics
Nucleic Acids
- Consistent, high-quality nucleic acid recovery
- Wide range of sample types
- Fast, automation-ready protocols with minimal hands-on time
Immunoprecipitation
- Low background – little to no nonspecific binding, and no preclearing and high signal to noise ratio
- Highly reproducible
- Fast, automation-ready protocols
- Antibody savings
- Highly sensitive
Protein
- Variety of assays and procedures based on affinity purification
- Rapid processing of samples
- Compatible with many downstream applications
- Fast, automation-ready protocols
Nucleic Acid Protocols
- Protocols designed for nucleic acid purification procedures and reagent kits
IP/Protein Protocols
- Protocols for Immunoprecipitation, Protein A, G, Sheep Anti-Mouse, Sheep Anti-Rabbit, Streptavidin
BindIT Software
Design or customize your own protocols
- Create and store protocols in a PC database
- Compatible with all KingFisher instruments
- Easy to use
- Generate reports
KingFisher Duo Prime
- 96 Deep-Well plate
- 24 Deep- Well plate
- 96 KF plate
- Elution strip
- Cap for elution strip
- 12-Tip comb
- 6-Tip comb
- Combi pack for 96 samples
KingFisher Flex
- 96 Deep-Well plate
- 24 Deep- Well plate
- 96 KF plate
- CapMat for 96DW plate
- 96-Tip comb for DW magnets
- 96-Tip comb for KF magnets
- 96-Tip comb for PCR magnets
- 24 Deep-Well Tip comb and plate
KingFisher Presto
- 96 Deep-Well plate
- 24 Deep- Well plate
- 96 KF plate
- 96-Tip comb for DW magnets
- 24 Deep-Well Tip comb and plate
Nucleic Acids
- Consistent, high-quality nucleic acid recovery
- Wide range of sample types
- Fast, automation-ready protocols with minimal hands-on time
Immunoprecipitation
- Low background – little to no nonspecific binding, and no preclearing and high signal to noise ratio
- Highly reproducible
- Fast, automation-ready protocols
- Antibody savings
- Highly sensitive
Protein
- Variety of assays and procedures based on affinity purification
- Rapid processing of samples
- Compatible with many downstream applications
- Fast, automation-ready protocols
Nucleic Acid Protocols
- Protocols designed for nucleic acid purification procedures and reagent kits
IP/Protein Protocols
- Protocols for Immunoprecipitation, Protein A, G, Sheep Anti-Mouse, Sheep Anti-Rabbit, Streptavidin
BindIT Software
Design or customize your own protocols
- Create and store protocols in a PC database
- Compatible with all KingFisher instruments
- Easy to use
- Generate reports
KingFisher Duo Prime
- 96 Deep-Well plate
- 24 Deep- Well plate
- 96 KF plate
- Elution strip
- Cap for elution strip
- 12-Tip comb
- 6-Tip comb
- Combi pack for 96 samples
KingFisher Flex
- 96 Deep-Well plate
- 24 Deep- Well plate
- 96 KF plate
- CapMat for 96DW plate
- 96-Tip comb for DW magnets
- 96-Tip comb for KF magnets
- 96-Tip comb for PCR magnets
- 24 Deep-Well Tip comb and plate
KingFisher Presto
- 96 Deep-Well plate
- 24 Deep- Well plate
- 96 KF plate
- 96-Tip comb for DW magnets
- 24 Deep-Well Tip comb and plate
How KingFisher technology works
The Thermo Scientific KingFisher Purification System utilizes permanent magnetic rods and disposable tip combs. When the magnetic rod–sheathed inside the tip comb–is lowered into the solution, magnetic beads collect at the bottom of the tip comb. The tip comb can then be positioned in a different row or plate, and the beads are then released by moving the magnetic rods out of the tip comb. The tip comb also facilitates the mixing of reagents with the beads by moving up and down.
Diagram details the KingFisher workflow.
Watch the video for a detail view of the workflow
KingFisher Videos
Automated Immunoprecipitation in 40 min using Dynabeads and KingFisher Flex
For IP, KingFisher instruments with Dynabeads offer the best balance of high yield and reproducibility with low nonspecific binding and cost, which is one reason why they have become the gold standard for immunoprecipitation using magnetic beads.
KingFisher Duo Prime
Greater consistency, better quality in less steps.
KingFisher Presto Sample Purification System
Resources
Brochure
Application Notes
Technical Notes
Smart Note
For Research Use Only. Not for use in diagnostic procedures.
