One of the most difficult viruses to extract is HBV due to the rigid protein coat. Sensitive detection requires a strong lysis and pure DNA. To look at the ability of the PureLink Viral RNA/DNA Mini Kit to efficiently extract HBV virus, samples of infectious patient plasma were extracted side by side with plasma spiked with ZeptoMetrix's HBV nucliec acid test control (NATtrol). HBV was detected in plasma samples diluted 1:100 from both patients (grey bars) and in NATtrol spiked samples (blue bars) (Figure 1).
|Figure 1.Equivalent Recovery of HBV in Patient Samples and Plasma Spiked with NATtrol™ Nucleic Acid Test Controls by ZeptoMetrix using PureLink Viral RNA/DNA Mini Kit. HBV was extracted from infectious plasma containing 3 x 109 IU/mL and from ZeptoMetrix nucleic acid test standard (NATtrol) for HBV containing 4 x 109 IU/mL using the PureLink Viral RNA/DNA Kit. Recovery of HBV NATtrol was comparable to infectious virus in patient plasma. qPCR was performed using the Platinum SYBR Green qPCR SuperMix-UDG w/ROX. NATtrol spiked samples (grey bars), HBV infected plasma (blue bars).
Data provided by Carleen Pope, M.S. Senior Research Scientist, ZeptoMetrix Corporation. Visit www.zeptometrix.com for more information on NATtrol products.
For Research Use Only. Not for use in diagnostic procedures.