BioPrime® Total FFPE Genomic Labeling System
- Improved call rates and less channel bias with enhanced Alex Fluor® 3 and 5 dye formulation for FFPE samples in aCGH experiments
- Representative results from FFPE samples using enzymatic RPA method
- Eliminate complicated volume-reduction steps with Purelink® purification included
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Improved call rates and reduced channel bias with FFPE samples
Representative results using enzymatic RPA method
With a random prime amplification (RPA) method, using labeling mixes specifically formulated for FFPE samples, BioPrime® Total FFPE Genomic Labeling System generates high yields of labeled DNA which is most representative of the starting sample. Increased call accuracy is achieved with higher yields of labeled material on the array, giving you more accurate calls in your aCGH experiments.
Eliminate complicated volume-reduction steps
Comparing PureLink™ (Invitrogen) and Microcon (Agilent) indicates that the majority of the free nucleotides are removed with PureLink™ purification. In addition PureLink™ also produces more higher molecular weight reaction products. The labeling data (not shown) further indicated that there is no purification bias for labeled and unlabeled nucleotides, as the DOL was not effected by two purification methods.
Figure 1. TBE Urea Gel of aCGH Labeling Kinetics.
Reaction products were separated by electrophoresis in 6% TBE-Urea gels and visualized on a Typhoon scanner/imager (Applied Biosystems) using the fluorescein channel (MW markers) and Cy/AF channels (labeling reaction products).
Lane 1: Purelink™ Normal Lane 3: Microcon® Normal
Lane 2: Purelink™ MCF7 Lane 4: Microcon® MCF7
Lane 7: Purelink™ Normal Lane 9: Microcon® Normal
Lane 8: Purelink™ MCF7 Lane 10: Microcon® MCF7
Lane 5&6: BioVentures fluorescein labeled Map Marker