artistic impression of researcher manually editing a DNA at the level of a nucleotide

Knock-in genome editing, simplified

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Easily create accurate and more successful knock-in experiments with TrueDesign Genome Editor. Our free design tool has an intuitive point-and-type interface that provides unrivaled flexibility and support for creating genome edits. The simple step-by-step program allows you to:

  • Generate a complete knock-in design in minutes
  • Edit up to 30 bases in any human gene using CRISPR/Cas9 or TALENs to create SNP or amino acids changes.
  • Add a GFP, RFP, or luciferase tag and create adaptor primers for our TrueTag™ Donor DNA Kits  to produce a donor template without cloning
  • Single-click download comprehensive documentation

Read how improved design and delivery of gRNA, Cas9 nuclease, and donor DNA contribute to enhanced CRISPR/Cas9-mediated genome editing

Genome editing workflow with the TrueDesign Genome Editor

Search for your gene

Screenshot of search interface for finding genes and related transcripts to edit within the human genome

Easily navigate the human genome to find the exact transcript and loci you want to modify.

Craft your edit

Screenshot of interface for specifying edits for given nucleic acid and amino acid sequences of a gene on interest

View the DNA and amino acid sequences while editing your gene with point-and-type features.

Complete your design

Screenshot of display and download options for CRISPR-Cas9 gRNA or TALEN designs as well as donor sequences

TrueDesign will generate your specific donor and the associated CRSIPR-Cas9 gRNA or TAL effector nucleases sequences.

Search for your gene

Screenshot of search interface for finding genes and related transcripts to edit within the human genome

Easily navigate the human genome to find the exact transcript and loci you want to modify.

Craft your edit

Screenshot of interface for specifying edits for given nucleic acid and amino acid sequences of a gene on interest

View the DNA and amino acid sequences while editing your gene with point-and-type features.

Complete your design

Screenshot of display and download options for CRISPR-Cas9 gRNA or TALEN designs as well as donor sequences

TrueDesign will generate your specific donor and the associated CRSIPR-Cas9 gRNA or TAL effector nucleases sequences.

Looking for the GeneArt CRISPR Search and Design Tool? This tool can be used to design gRNAs and compatible oligos for our Precision gRNA Synthesis Kit. If designing a variety of knock-in edit choices though, we recommend the NEW TrueDesign Genome Editor.

Before getting started, ensure the following:

1).  Is your browser supported? Thermo Fisher Cloud includes the latest data security and display features that require the use of one of the following browsers/versions:

Click on a link above to download and install a compatible browser. Thank you for using Thermo Fisher Cloud.

2). Once you are in a supported browser, you will need to log in one time only to the Thermo Fisher Cloud using your existing username and password. After your first visit to the TrueDesign Genome Editor, you will no longer be required to log in to the Cloud.

  • You will be asked to verify your email address the first time you log in.
  • A verification request email will be sent to the email address on file for your online account.
  • Check your spam folder if you don’t immediately receive this verification request.
  • Future visits to the TrueDesign Genome Editor will not require Thermo Fisher Cloud login.
  • Be sure to bookmark the page for future reference.