Maximize your success with CRISPR and TALEN technologies

From general overviews of CRISPR and TALEN-based genome editing techniques and experimental design best practices to specific applications including cell engineering and knock-in/-out strategies, our collection of videos and webinars is designed to help you maximize your success using these technologies.

Genome Editing Video Collection

Genome Editing Webinars

The genome editing webinar series is designed to help educate you in the latest genome editing technologies. Each webinar provides background information, data analysis, and helpful tips from our experienced technical support scientists and collaborators. Simply select from the list below to register for a live webinar, or one of our previously recorded webinars at your leisure. These webinars have something to offer everyone from novice to expert.

Master the Art of CRISPR Editing

In this free three-part webinar series you will learn tips and tricks for CRISPR delivery, how to best isolate edited clones, and downstream detection and validation methods for successful CRISPR gene editing.

Part 1—The art of CRISPR design
Key learning objectives (30 minutes):

  • gRNA design basics
  • Understanding available gRNA and donor DNA formats
  • Optimizing knockout experiments
  • Designing the optimal gRNA and donor DNA for knock-in experiments
  • Setting up a successful gene editing experiment

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Part 2—The art of optimizing CRISPR delivery 
Key learning objectives (30 minutes):

  • Understanding available CRISPR-Cas9 formats
  • Factors affecting CRISPR-Cas9 delivery
  • Optimizing delivery to maximize gene editing
  • Delivery options based on cell type and downstream application, including loss-of-function screening

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Part 3—The art of CRISPR detection, clonal isolation, and validation
Key learning objectives (30 minutes):

  • Optimizing conditions for CRISPR-mediated cell engineering
  • Quantifying editing efficiency and screening positive clones
  • Isolating and validating CRISPR-edited clones
  • Screening for off-target effects

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How to boost your CRISPR editing efficiency

CRISPR-Cas9 is rapidly evolving as the tool of choice for genome editing in mammalian cells. The design and synthesis of guide RNA (gRNA), as well as the difficulty of delivering Cas9 into various cell lines, are steps that limit overall efficiency and general ease of use. In this webinar, we describe a new state-of-the-art online CRISPR gRNA design tool that allows for rapid identification of gRNAs that have low off-target potential.

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For Research Use Only. Not for use in diagnostic procedures.