CRISPR libraries for functional genomics screening

CRISPR screening allows you to perform focused loss-of-function analyses on relevant gene sets or custom collections, or unbiased genome-wide CRISPR screens with confidence.


LentiArray and LentiPool gRNA libraries for CRISPR screening

Expand your CRISPR screening capabilities with lentiviral gRNA libraries. Lentiviral libraries make it easy to perform CRISPR gene edits for hundreds of genes at once. Award-winning Invitrogen LentiArray and LentiPool CRISPR libraries employ advanced gRNA designs for maximum knockout efficiency without sacrificing specificity. 

Lentiviral library formats

LentiArray CRISPR libraries

Delivered in arrayed plate format with one gene target (and up to four gRNAs) per well. Compatible with high-throughput screening platforms.

Two images showing difference in format between LentiArray CRISPR library and Lentipool CRISPR library

LentiPool CRISPR libraries

Collections of gRNA lentiviruses pooled together in a single tube. Empower CRISPR-Cas9 screening without the need for high-throughput infrastructure.

Two images showing difference in format between LentiArray CRISPR library and Lentipool CRISPR library

CRISPR libraries are constructed using our proprietary gRNA design algorithm, which incorporates the latest research and our extensive in-house experience. The gRNA designs are selected for maximum knockout efficiency without compromising specificity. For each gene target, we include up to four high-quality gRNAs to help ensure that the lentiviral library will provide high-efficiency knockout of target genes across a wide array of cell types.

LentiArray CRISPR libraries for high-throughput arrayed CRISPR screening

LentiArray CRISPR libraries are delivered in an arrayed format in 96-well plates, compatible with high-throughput screening platforms. These lentiviral libraries provide a flexible system that does not impose limitations on your functional genomics assay design or research goals. 

Data: LentiArray CRISPR libraries achieve high gene editing efficiency for most targeted genes

Images of a graph and Western blots showing that editing efficiency was high for cells transduced with LentiArray CRISPR Library gRNAs
Figure 1. LentiArray CRISPR Library gRNAs achieve high editing efficiencies for a large percentage of targeted genes.(A) HT1080 cells stably expressing Cas9 were transduced with arrayed LentiArray gRNA lentiviral particles against a subset of genes found in the LentiArray Human Cancer Biology CRISPR Library. NGS analysis showed that 87% of the targeted genes were knocked out at >50% efficiency (red line) and 77% at >70% efficiency (purple line). (B) U87MG and A431 cells stably expressing Cas9 were transduced with individual lentiviral gRNAs, then harvested for western blot analysis using specific antibodies against corresponding target genes. The absence of detectable AKT, PIK3R1, and EGFR proteins demonstrates efficient protein knockout by this method.


LentiPool CRISPR libraries for affordable screening

LentiPool CRISPR Libraries empower CRISPR-Cas9 screening without the need for high-throughput infrastructure. These high-quality pooled lentiviral libraries cover the same gene targets as our LentiArray libraries and are delivered as ready-to-use high-titer (>1 x 108 TU/mL) lentivirus particles for a gene knockout screen with maximum impact.

The LentiPool CRISPR libraries include lentiviruses that encode up to four gRNAs for each targeted gene, pooled together in one tube. All analysis can be performed using next-generation sequencing (NGS). Our LentiPool libraries are quality controlled by NGS to confirm gRNA and gene representation.


Pooled CRISPR library screening workflow

Diagrammed steps of workflow for utilizing the LentiPool gRNA library for pooled CRISPR screening

Generation of Cas9 expressing cells:

  1. Lentiviral transduction of cells with LentiArray Cas9 lentiviral particles
  2. Selection with blasticidin
  3. Expansion of resistant cells
Diagrammed steps of workflow for utilizing the LentiPool gRNA library for pooled CRISPR screening

Transduction with pooled sgRNA library

  1. Lentiviral transduction of Cas9-expressing cells with LentiPool sgRNA library
  2. Selection with puromycin
  3. Expansion of resistant cells
Diagrammed steps of workflow for utilizing the LentiPool gRNA library for pooled CRISPR screening

Primary screening by positive or negative selection: 

  1. Positive selection (+): Apply treatment to cells (e.g., drugs, chemical perturbant)
  2. Negative selection (–): Divide cells into reference and experimental samples
  3. Apply selective pressure or treatment only to experimental sample
Diagrammed steps of workflow for utilizing the LentiPool gRNA library for pooled CRISPR screening

Hit identification by NGS

High-throughput sequencing analysis of enriched [positive selection (+)] or depleted [negative seletion (–)] sgRNA from genomic DNA

Figure 2.Workflow for lentiviral pooled screening. A Cas9-expressing stable cell line is generated with LentiArray Lentiviral Cas9 Nuclease using selection for blasticidin resistance. These Cas9-expressing cells are then transduced with the LentiPool sgRNA library at the appropriate MOI and undergo selection for puromycin resistance. The transduced population then undergoes selective pressure or treatment like a drug or chemical perturbant. Genomic DNA is isolated and the sgRNA inserts are amplified by PCR. Finally, the amplicons then are sequenced to determine which genes were enriched/depleted in response to the treatment.



Positive, negative, and delivery controls for CRISPR screening

High-quality controls play an integral role in the successful development and performance of high-throughput screens. Don't forget to order controls to help you optimize delivery conditions, maximize editing efficiency, and establish hit selection criteria.

Specific to LentiArray CRISPR libraries, we offer a set of negative or positive delivery control options that can come with or without a GFP-expressing construct. The GFP marker can provide a visual readout to aid in the rapid optimization of viral delivery conditions.


Order LentiArray and LentiPool CRISPR libraries

These CRISPR libraries include up to four gRNAs per gene target with one gene per well. Lentiviral libraries are available in two formats:

  • 100 μL (2 x 50 μL) of ready-to-use lentiviral particles per gene target with an average titer of 1 x 108 TU/mL
  • Glycerol stocks delivered as 50 μL per gRNA, which can be used as a repository to generate your own pooled or arrayed libraries. Please note that use of this format requires plasmid preparation and lentiviral packaging.

Both predefined and custom CRISPR libraries are available, ranging from focused gene sets to whole-genome libraries. Our druggable genome CRISPR library is designed for identifying potential therapeutic targets involved in the development and progression of diseases.

The gene targets in each CRISPR library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and were cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary design algorithm developed by the scientists at Thermo Fisher Scientific. 


Available CRISPR Cas9 screening libraries

CRISPR library and descriptionGene count*gRNA count*LentiArray format
Ready-to-use particlesGlycerol stocks
Whole Genome CRISPR Library
Designed for genome-wide CRISPR screens to identify novel targets in biological pathways and disease development.
18,39273,568A42234A32167
Druggable Genome CRISPR Library
Designed for identification of potential therapeutic targets involved in the development and progression of disease.
10,13240,512InquireA32184
Kinase CRISPR Library
With kinases involved in many signaling cascades and their dysregulation linked to disease development, kinases are a key class of drug targets. Additional gene targets were selected from the KinBase database and other resources.
8223,288A42234A32167
GPCR CRISPR Library
As regulators of many physiological and disease processes, G protein-coupled receptors (GPCRs) have emerged as one of the most druggable gene classes, with nearly 30% of FDA-approved drugs targeting them.
4461,784A42282A32183
Cancer Biology CRISPR Library
Targets some of the most common genes involved in cancer development. Additional gene targets were select from the Cancer Genome Atlas (TCGA).
5102,040A42268A32169
Epigenetics CRISPR Library
Epigenetic regulation of gene expression plays a central role in normal development and is being recognized as a contributing factor to the development of many diseases.
3961,548A42269A32170
Ubiquitin CRISPR Library
The ubiquitin system is integral to maintaining cellular homeostasis by regulating protein turnover, and its dysregulation is linked to cancer, viral infection, and neurodegenerative, musculoskeletal, cardiovascular, and metabolic diseases.
9433,722A42270A32171
Cell Cycle CRISPR Library
Cell cycle regulators are important to normal development as well as cancer and cardiovascular, inflammatory, and neurodegenerative diseases. Targets include cyclin-dependent kinases (CDKs), crucial for cell cycle progression; cell cycle progression regulators such as CIP/KIP family proteins and INK4 cell cycle inhibitors; proteins in the retinoblastoma family; and DNA replication factors such as the cell division cycle proteins (CDCs).
1,4445,776A42271A32172
Membrane Trafficking CRISPR Library
Membrane trafficking proteins are involved in neurotransmitter and endocrine release, phagocytosis, endocytosis, autophagy, and other processes.
141564A42272A32173
Transcription Factor CRISPR Library
Transcription factors are key regulators of gene expression.
1,8177,724A42273A32174
Nuclear Hormone Receptors CRISPR Library
Nuclear hormone receptors are a family of transcription factors that are activated by lipid-soluble ligands such as steroid hormones, thyroid hormone, vitamin D, and retinoic acid, and regulate metabolism, development, proliferation, reproduction, and other biological processes.
47188A42274A32175
Apoptosis CRISPR Library
Apoptosis is a tightly regulated process that is essential for maintaining homeostasis in multicellular organisms. Inhibition of apoptosis can result in cancer, autoimmune and inflammatory diseases, and viral infection, while overactivation can lead to atrophy, tissue damage, and neurodegenerative disease. 
9043,616A42275A32176
Drug Transporter CRISPR Library
Transporter proteins play a key role in pharmacology, affecting how molecules are moved across cellular membranes.
98392A42276A32177
Ion Channel CRISPR Library
Ion channels are integral membrane proteins that establish the electrochemical gradient that leads to both resting membrane potential and the formation of action potentials. These are crucial to nerve conduction, heart and muscle contraction, pancreatic insulin release, T cell activation, and other processes.
3281,312A42277A32178
Cell Surface Protein CRISPR Library
The broad array of cell surface proteins allows cells to receive information from and react to their environment.
778

3,112

A42278A32179
Protease CRISPR Library
Besides degrading proteins, proteases are also integral signaling molecules, Dysregulation of protease signaling pathways is implicated in cardiovascular disease, neurological disorders, cancer, and inflammatory disease.
4751,900A42279A32180
Tumor Suppressor CRISPR Library
Tumor suppressors are negative regulators of cell cycle progression, and loss or mutation of tumor suppressor genes is often involved in cancer. Additional targets were selected from the Tumor Suppressor Gene Database (TSGene).
7162,864A42280A32181
DNA Damage Response CRISPR Library
DNA surveillance proteins continuously monitor DNA integrity and activate cell cycle checkpoints and DNA repair pathways in response to DNA damage.
5612,244A42281A32182
Phosphatase CRISPR Library
Reversible phosphorylation is central in regulating signal transduction pathways. Phosphatases dephosphorylate their target proteins, are integral to regulation of signaling pathways, and are potential therapeutic targets.
2881,152A42267A32168
Custom CRISPR LibraryCustomCustomInquireInquire

*Gene count and gRNA count subject to change

For Research Use Only. Not for use in diagnostic procedures.