photo of a Countess II FL Automated Cell Counter

Fluorescence with color options

With the option for a reusable slide and fluorescence capabilities—brightfield and two user-changeable fluorescence channels—the Invitrogen™ Countess™ II FL Automated Cell Counter can count cells, monitor fluorescent protein expression, and measure cell viability.

  • Affordable—option for reusable slide to significantly reduce consumables cost
  • Accuracy—eliminate the subjectivity of manual cell counting and minimize user-to-user variability
  • Speed—count live and dead cells and measure viability and average cell size in as little as 10 seconds

Reusable or disposable slides

The mounting costs of disposable slides and tips have deterred many labs from giving up tedious manual cell counting. In response to this, the Countess II FL instrument was designed to work with a reusable glass slide , which significantly reduces the long-term consumable costs of automated counting. For researchers who want the convenience of disposable slides, the Countess II FL instrument can also use the same PMMA plastic slides developed for the original Countess Cell Counter.

photo of the reusable glass slide

The Countess II FL Automated Cell Counter

 

"It outperformed all other competitor's models that we tried."Michael DaCosta, The Jackson Laboratory

Full spectrum of fluorescence detection—not limited to GFP and RFP

The Countess II FL instrument has two fluorescence channels. The fluorescent colors collected are determined by the insertion of individual light cubes. With 20 light cubes to choose from, the Countess II FL instrument is not limited to detection of GFP- and RFP-expressing cells. Looking to measure the transfection efficiency of cells transduced with an RFP-expression construct with a blue nuclear counterstain? No problem. Simply add the RFP and DAPI light cubes, and you can effectively count the number of cells expressing the RFP fusion within the total population and accurately determine the transfection efficiency.

A Texas Red light cube is available for optimal analysis of mCherry expressing cells, a very popular and commonly expressed fluorescent protein. To obtain viability information for these cells, simply utilize one of the Live/Dead fixable dyes that are offered in a range of colors to meet the application need. This spectrum of flexibility is possible using the user-interchangeable EVOS light cubes with the Countess II FL (Figure 1).

screenshot showing two-color fluorescent cell counting on the Countess II FL

 
Figure 1. Detection of GFP- and Texas Red-expressing cells.
Live and heat-killed cells were mixed, and then stained with calcein AM and ethidium homodimer supplied with the LIVE/DEAD Viability/Cytotoxicity Kit. The resultant solution was then evaluated using the GFP and Texas Red light cubes with the Countess II FL automated cell counter.

User-changeable light cubes

Central to the fluorescence detection technology inside the Countess II FL instrument are the proprietary EVOS™ LED light cubes. Each cube contains an LED, illuminating optics, and filters. Light cubes are user-changeable and auto-configured by the Countess system with plug-and-play capability . Our wide selection provides flexibility for multiple fluorescence research applications, such as cell viability analysis. See the entire line of compatible EVOS light cubes and compatible fluorescent dyes.

photo of 3 EVOS light cubes


Countess II FL Automated Cell Counter information

Technical specifications
  • Processing time: <10 seconds
  • Sample concentration range: 1 x 104–1 x 107 cells/mL
  • Particle diameter range: ~4–60 µm
  • Cell diameter range: ~7–60 µm
  • Required sample volume: 10 μL
  • USB drive: 4 gigabyte, FAT file system
  • Optics: 5 megapixels, 2.5X optical magnification, 3 channels (bright-field plus 2 slots for EVOS LED light cubes)
Physical characteristics
  • Instrument dimensions: 
    9" (W) x 5½" (D) x 9" (H); 228.6 mm (W) X 139.7 mm (D) x 228.6 mm (H)
  • Weight: 8 lbs
Counting area
  • Area: 2.15 mm x 1.62 mm (3.48 mm2)
    graphic showing larger counting area of Countess II FL compared to hemocytometers
Included in the box
  • Countess USB drive for storing and transferring data to your computer
  • Disposable slide holder
  • Reusable slide holder
  • Light cube removal tool
  • Power cord and 4 plug adaptors to accommodate most countries
  • Quick reference card (QRC)

Sold separately:

Researchers use Invitrogen Countess II and Countess II FL Cell Imaging Systems in their labs every day. Here's what some of our users have to say:

"Fast count and nice instruments, easy to use!! I love the small counter!"Dr. Xin Xu, Baylor College of Medicine

"It's very easy to use and it also allows me to quickly update my lab notebook so that each experiment uses the same number of cells. Quick and efficient."Lakeisha Tillery, Meharry Medical College

"It outperformed all other competitor's models that we tried."Michael DaCosta, The Jackson Laboratory

"It's very consistent!"Kim Sylvia, Sanford Burnham Medical Research Institute

"It is very effective at counting cells. It works very well."Mairead Bretney, Sanford Burnham Medical Research Institute

"The Countess II makes it very easy to count cells accurately and quickly - the fact that it tells you the dead cell and live cell count is icing on the cake."Faryal Mir, MIT

"We compared to a hemocytometer and the Countess II always obtained very close results. The fluorescent measurements are a great tool for us to quantify the % transfection and transduction efficiency in our experiments. The light cubes used to detect fluorescence are very easy to switch out when more than two fluorescence sources are needing to be detected. The automatic focusing is very effective and accurate with the option to manually focus."David Waynar, Santa Cruz Biotechnology

"This equipment is very useful for the purposes it is used in our lab. We love it!"Kristin Harwood, BioMarker Strategies, LLC

"Great for a quick check of transfection/transduction efficiency. Easy to use and seems pretty sensitive."Alex Peister, Morehouse College

"Great device. Broad range of cell densities that it can handle. Good at distinguishing clumps and counting cells within clumps."Joanna Rzepka, Veterinary Medical Research & Development

"Low maintenance, fast easy accurate cell counts. Easy for students to use and comprehend."Robert Dean, D'Youville College

"A very accurate cell counter and you can use it to count fluorescent cells as well. Excellent product. I'm very happy with it. I highly recommend it."Massimo Ammirante, University of California, San Diego

The result, image, and data files can be easily exported to a USB drive. The result and image files can be saved as .bmp, .jpg, .tiff, or .png files. The data file is saved as a .csv file that can be easily imported into your current analysis software or viewed in Excel™ software.

A comma-separated values (CSV) file stores tabular data (numbers and text) in plaintext form. Plain text means that the file is a sequence of characters, with no data that has to be interpreted as binary numbers. A CSV file can be opened with any third party software or spreadsheet program. The table below describes the categories of the Countess II data saved as a CSV file and opened with a spreadsheet program.

Category Column Name Description
General A Number Sequential sample run number
B File Name Name of file
C Date & Time Date and time of sample run
D Mode BF-Brightfield or FL-Fluorescence
Trypan Blue/
Brightfield
E Total concentration Concentration of the entire sample
F Total cells counted Total number of cells counted in the sample
G Live concentration Concentration of just the “live” portion of the sample
H Live cells counted Total number of “live” cells counted
I Dead concentration Concentration of just the “dead” portion of the sample
J Dead cells counted Total number of “dead” cells counted
K Viability (%) Percent viability of the sample based on trypan blue staining
L Average size (μm) Average cell size in microns
Fluorescence M Cube 1 name EVOS light cube name in the first (top) position
N Cube 1 concentration Concentration of cells showing fluorescence in the first cube position
O Cube 1 (%) Percentage of the total cells in brightfield that show fluorescence in the first cube position
P Cube 1 cells counted Total number of cells counted in the first cube position
Q Cube 2 name EVOS light cube name in the second (bottom) position
R Cube 2 concentration Concentration of cells showing fluorescence in the second cube position
S Cube 2 (%) Percentage of the total cells in brightfield that show fluorescence in the second cube position
T Cube 2 cells counted Total number of cells counted in the second cube position
U Cube 1+2 concentration Concentration of cells showing fluorescence in the first and second cube positions combined
V Cube 1+2 (%) Percentage of the total cells in brightfield that show fluorescence in the first and second cube position combined
W Cube 1+2 cells counted Total number of cells counted in the first and second cube position combined
General Details X Focus value Focal position number
Y BF Light intensity Brightfield light intensity value from 0-100%
Trypan Blue/
Brightfield Count Parameters
Z Live Size min Minimum size of “live” cells in microns
AA Live Size max Maximum size of “live” cells in microns
AB Live Brightness min “Live” adjustment slider value for minimum brightness
AC Live Brightness max “Live” adjustment slider value for maximum brightness
AD Live Circularity “Live” adjustment slider value for circularity
AE Dead Size min Minimum size of “dead” cells in microns
AF Dead Size max Maximum size of “dead” cells in microns
AG Dead Bright min “Dead” adjustment slider value for minimum brightness
AH Dead Bright max “Dead” adjustment slider value for maximum brightness
AI Dead Circ “Dead” adjustment slider value for circularity
Fluorescence Count Parameters AJ Cube 1 Light intensity First (top) light cube light intensity value from 0-100%
AK Cube 2 Light intensity Second (bottom) light cube light intensity value from 0-100%
AL BF Size min Minimum size of “Brightfield” cells in microns
AM BF Size max Maximum size of “Brightfield” cells in microns
AN BF Brightness min “Brightfield” adjustment slider value for minimum brightness
AO BF Brightness max “Brightfield” adjustment slider value for maximum brightness
AP BF Circularity “Brightfield” adjustment slider value for circularity
AQ Cube 1 Brightness min First (top) light cube adjustment slider value for minimum brightness
AR Cube 1 Brightness max First (top) light cube adjustment slider value for maximum brightness
AS Cube 2 Brightness min Second (bottom) light cube adjustment slider value for minimum brightness
AT Cube 2 Brightness max Second (bottom) light cube adjustment slider value for maximum brightness

Counting has been successfully performed on the following cell lines using the Countess II FL Automated Cell Counter:

Cell Animal Organ
A431 Human Skin
Adipocytes Human Adipose-derived stem cells
Aortic smooth muscle Human Smooth muscle
CHO-M1WT2 Chinese hamster Ovary
CHSE Chinook salmon Embryo
COLO-205 Human Colon
COS-7 African monkey Kidney
HeLa Human Cervix
HepG2 Human Liver
HL-60 Human Blood
J774A.1 Mouse Blood
Jurkat Human Blood
K562 Human Bone marrow
MCF7 Human Breast
MRC-5 Human Lung
NIH/3T3 Mouse Embryo
PBMC Human Blood
PC-12 Rat Adrenal gland
Pulmonary artery endothelial Human Blood vessel
Pulmonary artery smooth muscle Human Smooth muscle
SF-21 Insect Ovary
U266 Human Blood
U2OS Human Bone
Umbilical vein endothelial Human Blood vessel
Whole lysed blood Human Blood

Ordering information

NOTE: The reusable slide (Cat. No. A25750) is only compatible with the Countess II FL instrument.