TaqMan® OpenArray® technology enables you to achieve the highest sample throughput for mid-density genotyping. In a single day, 24 TaqMan® OpenArray® Genotyping Plates can be run on the OpenArray® Real-Time PCR Platform to generate data for >70,000 genotypes.

  • High-Throughput Results with Ease: With the OpenArray® Real-Time PCR Instrument and OpenArray® AccuFill™ System you can process thousands of samples per day without robotics
  • Simple Workflow: Each TaqMan® OpenArray® Genotyping Plate comes preloaded with the TaqMan® Genotyping Assays of your choice—just add sample and master mix, cycle, and image
  • Experimental Flexibility: Choose any combination of predesigned and custom TaqMan® SNP Genotyping Assays and the OpenArray® Plate format that best meets your project needs
  • Reliable: Uses trusted TaqMan® MGB probe chemistry
  • Easy-to-Understand Pricing: No oligo setup fee or hidden costs

SNP Genotyping using the OpenArray® Real-Time PCR Platform expression workflow

Life Technologies provides different solutions to isolate and purify DNA.

Recommended Products

MagMAX™ DNA Multi-Sample Kits
MagMAX™ DNA Multi-Sample Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells. These purification kits use MagMAX™ magnetic bead-based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays, CE and next-generation sequencing, and high resolution melting analysis. MagMAX™ kits are available in both tube format (for manual processing) and in 96-well plate format (compatible with automated processing).

Select or design TaqMan® Assays using the TaqMan® OpenArray® Plate Configurator or Custom TaqMan® Assay Design Tool, respectively. TaqMan® OpenArray® Genotyping Plates are delivered with assays dried down in the through-holes you specify. Mix DNA samples with TaqMan® OpenArray® Genotyping Master Mix in a TaqMan® OpenArray® 384-well sample plate.

Recommended Products

 

Configure a Genotyping Plate
Configure TaqMan® OpenArray® Genotyping Plates using predesigned assays and/or existing custom assay designs from your workspace.

OpenArray® Master Mix, Loader Tips & other plastics:

Load sample mixes onto the TaqMan® OpenArray® Real-Time PCR Plate using the OpenArray® AccuFill™ System. Next, insert the TaqMan® OpenArray® Real-Time PCR Plate into the OpenArray® case filled with immersion fluid and seal with glue. Run the reactions and collect data using the OpenArray® Real-Time PCR Instrument.

Recommended Products

 

QuantStudio™ 12K Flex Open Array® Accufill™ System
The OpenArray® AccuFill™ System load the sample mix from a 384-well plate onto the TaqMan® OpenArray® Real-Time PCR plate.

OpenArray® Sample Tracker Software
OpenArray® Sample Tracker Software integrates the sample information from a 384-well plate file iwth the assay information in the plate setup file.

OpenArray® AccuFill System Tips

384 AccuFill™ Tips for Accurate Sample Loading

OpenArray® Accufill™ System Tips, 10 pack

Acquire, analyze, and manage data quickly and easily. Amplification curves are presented for review, and data is easily manipulated to calculate Ct and Tm, as well as ΔCt and ΔΔCt measurements.

Recommended Products

 

OpenArray® Real-Time PCR Platform
The OpenArray® Real-Time PCR Instrument and OpenArray® AccuFill™ System combination can process hundreds to thousands of samples per day, enabling mid-density, high-throughput results with ease of use and without robotics.

 Dual Flat Block GeneAmp® PCR System 9700
The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The Dual Flat Block GeneAmp® PCR System 9700 is designed for use with TaqMan® OpenArray® Genotyping Plates on the OpenArray Real-Time PCR System.

The data is plotted in an allelic discrimination plot with FAM™ fluorescence signal on the y-axis and VIC® fluorescence signal on the x-axis. Software tools enable converting the raw data into genotype calls (e.g., homozygotes and heterozygotes.)

Recommended Products

 

TaqMan® Genotyper Software
TaqMan® Genotyper Software is for use with TaqMan® SNP Genotyping Assays (Predesigned, Custom & DME) in combination with 48-, 96- & 384-well microtiter plates, and OpenArray® Genotyping Plates and offers a state-of-the-art genotype calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.

Step 1 : Prepare Sample

Life Technologies provides different solutions to isolate and purify DNA.

Recommended Products

MagMAX™ DNA Multi-Sample Kits
MagMAX™ DNA Multi-Sample Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells. These purification kits use MagMAX™ magnetic bead-based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays, CE and next-generation sequencing, and high resolution melting analysis. MagMAX™ kits are available in both tube format (for manual processing) and in 96-well plate format (compatible with automated processing).

Step 2 : Select Assays & Configure Plates

Select or design TaqMan® Assays using the TaqMan® OpenArray® Plate Configurator or Custom TaqMan® Assay Design Tool, respectively. TaqMan® OpenArray® Genotyping Plates are delivered with assays dried down in the through-holes you specify. Mix DNA samples with TaqMan® OpenArray® Genotyping Master Mix in a TaqMan® OpenArray® 384-well sample plate.

Recommended Products

 

Configure a Genotyping Plate
Configure TaqMan® OpenArray® Genotyping Plates using predesigned assays and/or existing custom assay designs from your workspace.

OpenArray® Master Mix, Loader Tips & other plastics:

Step 3 : Load Samples

Load sample mixes onto the TaqMan® OpenArray® Real-Time PCR Plate using the OpenArray® AccuFill™ System. Next, insert the TaqMan® OpenArray® Real-Time PCR Plate into the OpenArray® case filled with immersion fluid and seal with glue. Run the reactions and collect data using the OpenArray® Real-Time PCR Instrument.

Recommended Products

 

QuantStudio™ 12K Flex Open Array® Accufill™ System
The OpenArray® AccuFill™ System load the sample mix from a 384-well plate onto the TaqMan® OpenArray® Real-Time PCR plate.

OpenArray® Sample Tracker Software
OpenArray® Sample Tracker Software integrates the sample information from a 384-well plate file iwth the assay information in the plate setup file.

OpenArray® AccuFill System Tips

384 AccuFill™ Tips for Accurate Sample Loading

OpenArray® Accufill™ System Tips, 10 pack

Step 4 : Cycle & Image

Acquire, analyze, and manage data quickly and easily. Amplification curves are presented for review, and data is easily manipulated to calculate Ct and Tm, as well as ΔCt and ΔΔCt measurements.

Recommended Products

 

OpenArray® Real-Time PCR Platform
The OpenArray® Real-Time PCR Instrument and OpenArray® AccuFill™ System combination can process hundreds to thousands of samples per day, enabling mid-density, high-throughput results with ease of use and without robotics.

 Dual Flat Block GeneAmp® PCR System 9700
The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The Dual Flat Block GeneAmp® PCR System 9700 is designed for use with TaqMan® OpenArray® Genotyping Plates on the OpenArray Real-Time PCR System.
Step 5 : Analyze Data

The data is plotted in an allelic discrimination plot with FAM™ fluorescence signal on the y-axis and VIC® fluorescence signal on the x-axis. Software tools enable converting the raw data into genotype calls (e.g., homozygotes and heterozygotes.)

Recommended Products

 

TaqMan® Genotyper Software
TaqMan® Genotyper Software is for use with TaqMan® SNP Genotyping Assays (Predesigned, Custom & DME) in combination with 48-, 96- & 384-well microtiter plates, and OpenArray® Genotyping Plates and offers a state-of-the-art genotype calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.

Literature

Ordering Guide: TaqMan® OpenArray® Genotyping Plates
Product Bulletin: OpenArray® Real-Time PCR System
Quick Reference Card: TaqMan® OpenArray® Genotyping System Workflow

Access to OpenArray™ publications search ›