SNP Genotyping Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block
TaqMan® OpenArray® technology enables you to achieve the highest sample throughput for mid-density genotyping. In a single work day, 36 TaqMan® OpenArray® Genotyping Plates can be run on the OpenArray® Real-Time PCR Platform to generate data for >110,000 genotypes.
- High-Throughput Results with Ease
The QuantStudio™ 12K Flex Real-Time PCR System and QuantStudio™ 12K Flex OpenArray® AccuFill™ System help enable you to process thousands of samples per day without robotics.
- Simple Workflow
Each QuantStudio™ 12K Flex OpenArray® plate, encased in an alloy bottom for easy handling, comes preloaded with the assays of your choice—just add sample and master mix, cycle and image.
- Best Experimental Flexibility
Choose any combination of pre-designed and custom TaqMan® SNP Genotyping Assays and the sample/SNP format that best meets your project needs.
- Reliable and economical
Uses gold standard TaqMan® MGB probe chemistry in a nanofluidic design that reduces reagent usage
- Easy-to-Understand Pricing
No oligo setup fee or hidden costs.
Experimental workflow: find the products you need for every step
Isolate and purify genomic DNA.
Products for Step 1: Prepare Sample
MagMAX™ DNA Multi-Sample Kit
Select or design TaqMan® probes using the TaqMan® OpenArray® Plate Configurator or Custom TaqMan® Assay Design Tool respectively. QuantStudio™ 12K Flex TaqMan® OpenArray® Genotyping Plates are delivered with assays dried down in the through-holes you specify. Mix DNA samples with TaqMan® OpenArray® Master Mix in TaqMan® OpenArray® 384-well sample plate.
TaqMan® OpenArray® Genotyping Plates
|Configure a Genotyping Plate
Configure TaqMan® OpenArray® Genotyping Plates using pre-designed assays and/or existing custom assay designs from your workspace
Design Your Own Custom Plate
Configure TaqMan® OpenArray® Genotyping Plates using the TaqMan® Custom Assay Design Tool
Load sample mixes onto QuantStudio™ 12K Flex TaqMan® OpenArray® Genotyping Plate using the QuantStudio™ 12K Flex OpenArray® AccuFill™ System. The plate is encased with an alloy bottom for easy handling. Simply apply the adhesive lid and fill with immersion fluid. You can then perform on-line cycling on the QuantStudio 12K Flex system. To increase your throughput even more, as an option, you can amplify your on the Dual 96-well GeneAmp PCR System 9700 and perform an endpoint plate read using the QuantStudio 12K Flex system; TaqMan® SNP Genotyping Assay MGB probes provide a fluorescence signal for the amplification of each allele.
The data is plotted in an allelic discrimination plot with FAM fluorescence signal on the y-axis and VIC fluorescence signal on the x-axis. Software tools enable converting the raw data into genotype calls (e.g., homozygotes and heterozygotes.)
TaqMan® Genotyper Software
For Research Use Only. Not for use in diagnostic procedures.