Overview

The Applied Biosystems QuantStudio 6 and 7 Pro real-time PCR systems deliver greater flexibility to support a variety of applications and throughput requirements. The systems’ smart features are designed to reduce contamination by allowing you to set up your run hands-free. Schedule and monitor your run from your mobile device, set-up your experiment, analyze data, and share your results from your computer no matter where it is or where you are.  
  

The QuantStudio 6 and 7 Pro systems’ 96-well blocks incorporate VeriFlex technology for precise temperature control. These blocks are divided into isolated zones that allow separate cycling conditions within the same run -- perfect for optimization. You can also increase your throughput with interchangeable blocks that accommodate 96-well 0.2 ml blocks, 96-well 0.1 ml blocks, 384-well blocks and TaqMan Array Cards (QuantStudio 7 Pro model only). Other unique features of the QuantStudio 7 Pro system include automation compatibility and software features to assist with 21 CFR Part 11 compliance. Many of these features can be added to the QuantStudio 6 Pro system through upgrades to meet changing lab requirements. 


Features

In addition to the common features of all QuantStudio systems, the QuantStudio 6 and 7 Pro systems offer:

Remote set-up, monitoring and sharing

Monitor experiments and access results remotely using the Instrument Connect app

Multiplexing

5 or 6 optical channels (21 filter combinations for QuantStudio 7 Pro system)

Interchangeable blocks

Flexibility to change block formats between 96-well (0.1 and 0.2 mL), 384-well, and TaqMan Array cards (QuantStudio 7 Pro model only) with ease by simply pushing a button or using a voice command

Smart features

Hands-free functionality includes facial authentication login, an RFID reader, and the ability to perform basic operations using voice commands

Innovative software

Data analysis applications that bring together multiple data sets in one convenient place and render them in stunning data visualizations for enhanced analysis and insights

Green features

Less waste and fewer resources (uses up to 54% less material than comparable instruments and generates 91% less waste during calibration); energy efficient (consumes up to 26% less energy)

Features that are unique to the QuantStudio 7 Pro system:

TaqMan Array cards

Use 5X less sample volume and load up to 384 targets with just 8 pipetting steps in less than 10 minutes. Available with QuantStudio 7 Pro system (upgrade option for the QuantStudio 6 Pro system)

Automation compatibility

Increase productivity and reduce your manual tasks by adding automatic loading with the pre-configured Thermo Scientific Orbitor RS2 Microplate Mover, or integration with other lab automation.

Software to assist with 21 CFR Part 11 compliance

Included with the QuantStudio 7 Pro system (upgrade option for the QuantStudio 6 Pro system)


Videos and demos

"It’s SO small. Impressed by the very user friendly touchscreen so we do not need a computer next to it… having it integrated into the cloud is quite convenient."

Mikael Kubista

TATAA Biocenter AB,

Sweden

"The facial authentification, voice commands, and super responsive and intuitive touch screen menu make it one of the few instruments that is actually very easy to use as a stand-alone instrument (rather than running the instrument through a computer).”

Richard Fekete,

PhD

NemaMetrix Inc

"The enhanced workflow and data management from the QuantStudio 6 Pro software and Connect. cloud-enabled systems will save hours upon hours in set-up and data transfer time. I can set up my run method while away from the lab, then just drop in my plate and go. Automatic data transfer to Connect has greatly simplified data management and sharing. The assurance that everyone on the team is seeing the same data and analysis will become extremely important as we scale up our qPCR operations.”

Wenlan Hu

NemaMetrix Inc


Quickly and easily change blocks (for example 96 to 384 wells) with no tools required. On-board software guides through a simple process.

The QuantStudio 7 Pro system can be used with the Orbitor RS2 mover, a microplate robot. This enables real-time PCR in automated environments for increased sample throughput and enhanced walkaway time.

Increase efficiency with TaqMan Array cards on the QuantStudio 7 Pro system. Load up to 384 targets with just 8 pipetting steps. Simply add sample and master mix—ready to go in less than 10 minutes. TaqMan Array cards use 5X less sample volume; ideal for low-expressing genes or precious samples.


Sample data

  • 1.5-fold discrimination
  • Comparison across Applied Biosystem real-time PCR systems
  • 10-log separation
  • Single copy detection
  • Multiplex reaction

Distinct clusters indicating 2-fold and 1.5-fold discrimination on QuantStudio 7 Pro system with 99.9% confidence, equivalent to other Applied Biosystems platforms, QuantStudio 7 Flex and the 7900HT instruments. Total liver cDNA samples with 2-fold and 1.5-fold differences in input were assessed. Runs were analyzed using their respective platform software, with auto-threshold and baseline, then exported and analyzed using SAS JMP v13 statistical software.

The QuantStudio 7 Pro system can detect 1.5 fold changes in expression. A dilution series of the KAZ plasmid was amplified. The vertical axis is the change in fluorescence normalized to the ROX dye. The horizontal axis is increasing cycle number from left to right. This is verified by plotting as a standard curve on the right. The vertical axis of the standard curve is the cycle threshold value, and the horizontal axis is the dilution series increasing in concentration from left to right.

Comparable results across QuantStudio 7 Pro system and other Applied Biosystems platforms when comparing different genes. To demonstrate equivalency of relative gene expression results using the new QuantStudio 7 Pro system compared to those using the QuantStudio 7 Flex and 7900HT instruments, three gene assays (GFAP, ALB, and KRT1) labeled with FAM-MGB and an endogenous control, PPIA (VIC-QSY, primer limited), were run on each platform. 

Highly reproducible separation over the 10 logs of input template amount demonstrated by amplification of KAZ plasmid DNA in 10-fold dilutions using the 96-well block was performed. The vertical axis is the log of the background subtracted fluorescence normalized to the ROX dye. The horizontal axis is increasing cycle number from left to right.

Single copy of the KAZ plasmid in blue compared to NTC in purple.  The QuantStudio 7 Pro system consistently detects a single copy, clearly distinguishable from NTCs (43/48 positive wells).

Multiplex reaction detects 18S rRNA labeled with VIC dye and TGFb  gene with FAM labeled TaqMan probes and primers. The reaction used the fast master mix and was run in 96 replicates. The vertical axis reflects the change in florescence normalized to the ROX dye, and the horizontal axis reflects increasing cycles from left to right. The CT values show a clear, consistent separation of products with a low standard deviation among replicates.


Instrument calibration and verification kits

Maximize the performance of your QuantStudio 7 Pro system with the following calibration kits.


Specifications

*Can be upgraded

 

 

For Research Use Only. Not for use in diagnostic procedures.