QuantStudio 3 and 5 real-tme PCR systems are factory-calibrated for quick installation and immediate use. These systems detect differences in target quantity as low as 1.5-fold, allow remote monitoring, and feature optional cloud-based Applied Biosystems analysis modules and data sharing.


Designed for both new and experienced users, they support 96-well (0.2ml or 0.1ml) or 384-well (QS5 only) consumables.  The systems also feature multiplexing capabilities for up to 6 targets and software to enable 21 CFR Part 11 compliance.

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Adaptable configurations

This compact instrument can be configured as a standalone or with a computer to fit any laboratory needs.

Pre-optimized protocol templates

Pre-optimized protocol templates minimize new user training 

VeriFlex blocks

Applied Biosystems 96-well blocks feature VeriFlex technology with three or six independent temperature zones for precise temperature control and optimization. 

QuantStudio 3 & 5 qPCR Data Analysis Software

Allows you to remotely monitor your runs, quickly analyze sophisticated data sets, store results in a secure space, and safely share them online 

More well options

96-well (0.1-mL block), 96-well (0.2-mL block), and 384-well options

Software to assist with 21 CFR Part 11 Compliance

Security, auditing, e-signature package included

Videos and demos

"We didn’t want to just create another instrument, we wanted to create the ecosystem."

Damien Luk

Thermo Fisher Scientific

San Francisco

“It’s important that I stay connected to the labs so that our people can progress with their projects, even while I’m away.”

Sample data

Precise quantification with 1.5-fold discrimination demonstrated by amplification plots for 1.5-fold dilutions of a KAZ plasmid and the standard curve generated from the CT values.

Real-time PCR reproducibility shown by results from amplification of KAZ target plasmid DNA in 10-fold dilutions using the 96-well block.

Melt curve analysis using the online version of the software for 96 replicates of human genomic DNA. 

Genotyping analysis demonstrated by allelic discrimination plot with traces using real-time PCR data for 44 gDNA samples and 4 no-template controls.

Multiplex reaction with four targets plus passive reference for whole-plate amplification plots of 96 replicates of cDNA made from universal human RNA (UHR). Specific to QuantStudio 5 system.

Instrument calibration and verification kits

Maximize the performance of your QuantStudio 3 and 5 systems with the following calibration kits. 


For Research Use Only. Not for use in diagnostic procedures.