QuantStudio 3 and 5 real-tme PCR systems are factory-calibrated for quick installation and immediate use. These systems detect differences in target quantity as low as 1.5-fold, allow remote monitoring, and feature optional cloud-based Applied Biosystems analysis modules and data sharing.


Designed for both new and experienced users, they support 96-well (0.2ml or 0.1ml) or 384-well (QS5 only) consumables.  The systems also feature multiplexing capabilities for up to 6 targets and software to enable 21 CFR Part 11 compliance.

Financing for as low as 4%* for a limited time only.  Learn more ›


Adaptable configurations

This compact instrument can be configured as a standalone or with a computer to fit any laboratory needs.

Pre-optimized protocol templates

Pre-optimized protocol templates minimize new user training 

VeriFlex blocks

Applied Biosystems 96-well blocks feature VeriFlex technology with three or six independent temperature zones for precise temperature control and optimization. 

QuantStudio 3 & 5 qPCR Data Analysis Software

Allows you to remotely monitor your runs, quickly analyze sophisticated data sets, store results in a secure space, and safely share them online 

More well options

96-well (0.1-mL block), 96-well (0.2-mL block), and 384-well options

Software to assist with 21 CFR Part 11 Compliance

Security, auditing, e-signature package included

Videos and demos

"We didn’t want to just create another instrument, we wanted to create the ecosystem."

Damien Luk

Thermo Fisher Scientific

San Francisco

“It’s important that I stay connected to the labs so that our people can progress with their projects, even while I’m away.”

Sample data

Precise quantification with 1.5-fold discrimination demonstrated by amplification plots for 1.5-fold dilutions of a KAZ plasmid and the standard curve generated from the CT values.

Real-time PCR reproducibility shown by results from amplification of KAZ target plasmid DNA in 10-fold dilutions using the 96-well block.

Melt curve analysis using the online version of the software for 96 replicates of human genomic DNA. 

Genotyping analysis demonstrated by allelic discrimination plot with traces using real-time PCR data for 44 gDNA samples and 4 no-template controls.

Multiplex reaction with four targets plus passive reference for whole-plate amplification plots of 96 replicates of cDNA made from universal human RNA (UHR). Specific to QuantStudio 5 system.

Instrument calibration and verification kits

Maximize the performance of your QuantStudio 3 and 5 systems with the following calibration kits. 


For Research Use Only. Not for use in diagnostic procedures.




*This offer is only available to customers in Canada and the United States (excluding Puerto Rico) who acquire manufactured products and service plans from Thermo Fisher Scientific or its affiliates. Some exclusions may apply. Offer will apply to approved applicants and who sign a Thermo Fisher Financial Services contract that is received by Thermo Fisher Financial Services no later than March 10, 2023. Customers must submit a Thermo Fisher Financial Services credit application and be approved for credit. Interest rates vary based on credit rating. Not all buyers may qualify for credit approval or promotional rate. Determination of eligibility is at the sole discretion of Thermo Fisher Financial Services credit committee. Financing shall be subject to contract terms and conditions. Cannot be combined with other discounts or promotions.  Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Offer subject to change without notice. Other restrictions may apply.