TaqMan Real-Time PCR Master Mixes

TaqMan™ Master Mixes contain buffer, dNTPs, passive reference dye, thermostable hot-start DNA polymerase, and other components formulated for reliable TaqMan™ Assay-based real-time PCR. Just add your sample and TaqMan Assay, and start your reactions.

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Which TaqMan master mix is right for you?

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  Gene Expression (Fast or Standard mode) Genotyping and copy number variation (CNV) (Fast and Standard mode)
  TaqMan™ Fast Advanced Master Mix TaqPath™ ProAmp™ Master Mix (GPR*)
Price / 20 µL reaction $0.81–$0.95 $0.58–$0.85
Application examples Gene expression for mRNA & small RNA SNP genotyping, mutation detection, crude lysate samples, and CNV
Cycling/run time 0:40 0:50 (fast) / 1:20 (standard)
UDG to prevent DNA carryover contamination Heat-labile UDG to prevent carryover contamination
ROX reference dye to normalize signal & ensure data integrity
Universal cycling for assay to assay consistency
Benchtop stability 72 hr 72 hr
Tolerant to PCR inhibitors

While Applied Biosystems™ TaqMan® Universal Master Mix II can be used for all applications, TaqPath™ ProAmp™ Master Mix has been specifically designed and will provide the best performance for genotyping applications (e.g., CNV, DME, and SNP).

* For laboratory use. General purpose reagent.

Other Applied Biosystems master mixes

TaqMan® PreAmp Master Mix is designed to facilitate analysis of limited-quantity samples, including single cells. Use the mix to expand small amounts of cDNA by pre-amplification without introducing amplification bias to the sample. Pre-amplification enables you to stretch your precious sample for use in many more real-time PCR reactions than you could otherwise run.

TaqMan® Environmental Master Mix 2.0 offers accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors. Use this master mix to analyze environmental, food, and other challenging samples.

TaqMan® Genotyping Master Mix is specifically formulated for detection of copy number, DME’s, and SNPs. Use this mix for the best performance for genotyping applications.

TaqMan® Fast Environmental Master Mix Beads have been designed to match or exceed the performance of standard (not fast) real-time PCR master mixes, delivering shorter run times than these master mixes (<40 minutes in fast mode) with an easy-to-use, lyophilized bead format. These beads are available with or without an Applied Biosystems™ VIC™ dye–labeled internal positive control (IPC).

For testing of Food and Environmental samples only.