Zika and Arbovirus Detection by Real-Time PCR

Simple-to-use solutions for dengue, chikungunya, and Zika virus detection: just add RNA

Zika virus is a single-stranded RNA arbovirus that is spread to humans primarily through the bite of an infected mosquito of the Aedes genus, causing Zika virus disease. Infected individuals may have fever, rash, joint pain, and/or conjunctivitis. Studies have shown that Zika virus can be passed from an infected pregnant woman to her unborn baby, and may be linked to birth defects.

For more up-to-date information on Zika virus and the current outbreak, visit the CDC website.

FDA-issued emergency use authorization (EUA) for TaqPath Zika Virus Kit**.
Available now.

The Applied Biosystems TaqPath Zika Virus Kit (0.1 mL format) is the first Zika virus test to be issued with Emergency Use Authorization that utilizes lyophilization technology, making it simple to use.

Order now 

Detection of Zika and other arboviruses

Although chikungunya, dengue, and Zika viruses can be detected by testing serum for the presence of viral nucleic acid or virus-specific immunoglobulin M (IgM) and neutralizing antibodies, serological cross-reactivity is strong between Zika and dengue viruses. Molecular detection using real-time PCR is strongly recommended.

See the CDC’s updated information on diagnostic testing for Zika, chikungunya, and dengue viruses in US public health laboratories.

Real-time PCR detection of Zika and arboviruses

Thermo Fisher Scientific offers three types of Zika and arbovirus real-time PCR solutions. Each of these kits offers the following:

  • Less than five minutes hands-on time
  • Easy to use–simply add RNA
  • No cold-chain management required; ship and store at room temperature
  • Lyophilized with one-step qRT-PCR master mix
  • TaqPath Zika Kit (EUA) optimized for the Applied Biosystems QuantStudio Dx Real-time PCR system
  • All other RUO TaqMan Zika and Arbovirus kits optimized for the Applied Biosystems QuantStudio 5 Real-Time PCR System; also suitable for the 7500, 7500 Fast, and other QuantStudio systems

The Zika virus sequence used to develop these primers and probes was identified in a 2014 research paper by Pyke et al., titled “Imported Zika Virus Infection from the Cook Islands into Australia, 2014”.

For viral RNA purification, we recommend use of the Applied Biosystems MagMAX Pathogen RNA/DNA Kit.

Read our Accelerating Science blog “Battling Zika – Do we have your attention now?".

Video: The State of Zika
Learn more about how scientists in Brazil work to find answers around the Zika virus, and how Thermo Fisher Scientific worked diligently to develop the Zika real-time PCR research assay.

Order our Zika and arbovirus products

To obtain a quote for any of our Zika and arbovirus products, please select the product(s) of interest and complete the form below. A sales representative will contact you.

Cat. no.DescriptionFormat
Singleplex assay format (EUA issued)
A31745TaqPath Zika Virus **(EUA issued)Fast, 0.1 mL block
Singleplex assay format  (RUO)
A31744TaqMan Zika Virus Kit (ZIKV)Standard, 0.2 mL block
Triplex assay format (RUO)
A31746TaqMan Zika Virus Triplex Kit (ZIKV/DENV/CHIKV)Standard, 0.2 mL block
A31747TaqMan Zika Virus Triplex Kit (ZIKV/DENV/CHIKV)Fast, 0.1 mL block
Vector screening (RUO)
A26105TaqMan Zika Virus Triplex Vector Screening Kit (ZIKV/DENV/CHIKV)Standard, 0.2 mL block
A31748TaqMan Zika Virus Triplex Vector Screening Kit (ZIKV/DENV/CHIKV)Fast, 0.1 mL block
 TaqPath Zika Virus Kit (0.1 mL, 12 x 8-well strip tubes, EUA issued)**
 TaqMan Zika Virus Kit (0.2 mL, 12 x 8-well strip tubes, RUO)
 TaqMan Zika Virus Triplex Kit (0.2 mL, 12 x 8-well strip tubes, RUO)
 TaqMan Zika Virus Triplex Kit (0.1 mL, 12 x 8-well strip tubes, RUO)
 TaqMan Zika Virus Triplex Vector Screening Kit (0.2 mL, 12 x 8-well strip tubes, RUO)
 TaqMan Zika Virus Triplex Vector Screening Kit (0.1 mL, 12 x 8-well strip tubes, RUO)

Related products for arbovirus (Zika) detection by real-time PCR

Perform real-time PCR (IVD)

QuantStudio Dx Real-Time PCR System

The Applied Biosystems QuantStudio Dx instrument provides your clinical laboratory with performance you can trust, security you demand, and flexibility you need in your workflow to meet the needs of your lab today and tomorrow.

For In Vitro Diagnostic Use. The QuantStudio Dx Real-Time PCR instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD-registered QuantStudio Dx Real-Time PCR System is for distribution and use in specific countries only.

Perform real-time PCR (RUO)

QuantStudio 5 Real-Time PCR System (96-well, 0.2 mL or 0.1 mL block)

The Applied Biosystems QuantStudio 5 Real-Time PCR System is ideal for all levels of users who need a high-performance instrument with features for maximum control of their experiments. Includes built-in security features, locked workflows, user account management, and options for Web browser–based or desktop analysis.

Visit thermofisher.com/quantstudio5 for more information.

Isolate viral RNA

MagMAX Pathogen RNA/DNA Kit

The Applied Biosystems MagMAX Pathogen RNA/DNA Kit enables purification of RNA and DNA from viruses and easy-to-lyse bacteria and parasites using magnetic particle technology in a 96-well format. PCR inhibitors are effectively removed in the process, making the resulting nucleic acid ideal for real-time PCR and RT-PCR applications.

** This test has been issued Emergency Use Authorization for TaqPath Zika Virus (ZIKV) Kit, 0.1 mL format by the US FDA. This test has been authorized by the FDA under an EUA for use by authorized laboratories;  this test has been authorized only for the detection of RNA from Zika virus and diagnosis of Zika virus infection, not for any other viruses or pathogens. This test is only authorized for the duration of the declaration that circumstances exist justifying the authorization of the emergency use of in vitro diagnostic tests for detection of Zika virus and/or diagnosis of Zika virus infection under section 564(b)(1) of the Act, 21 U.S.C. § 360bbb-3(b)(1), unless the authorization is terminated or revoked sooner. For full details including protocol and patient information, visit thermofisher.com/zikaeua.