Ion Proton™ Sequencer Specifications | Thermo Fisher Scientific

Key applications

ChIP sequencing
Exome sequencing
Gene expression sequencing
De novo sequencing
Small RNA sequencing
Whole transcriptome sequencing

Areas of interest

Agricultural research
Stem cell research
Cancer research
Epigenomics
Ancient DNA genomics
Metagenomics

Chip compatibility

Ion PI v3 Chip

60–80M reads
~2.5 hours run time

Ion Proton System performance specifications with Ion PI Chip

Throughput	Up to 15 Gb
Read length	Up to 200-base fragment reads
Number of reads passing filter	60-80 million reads passing filter
Sequencing run time	2.5 hours
Barcoding solutions	384 barcodes supported by Torrent Suite Software 96 off-the-shelf barcodes for DNA 16 off-the-shelf barcodes for RNA
Software solutions	Torrent Suite Software utilizing Torrent Browser including TMAP alignment and Torrent Variant Caller for germline or somatic mutation detection. Torrent Browser (included) offers users remote web access to instrument status monitoring, run quality reports, individual data files, and extensible plug-ins for application-specific analysis. Plugins available for download from the Torrent Browser Plugin Store for analysis of genome sequencing, targeted sequencing, variant analysis and annotation, microbial sequencing, transfer to 3rd party bioinformatics packages, and more. Data can optionally flow into Ion Reporter Software for controlled analysis, annotation, and reporting of variants. Ion Reporter Software provides application specific workflows to support single, trio and paired tumor normal analysis.
Data formats	Industry standard FASTQ, SFF, BAM and VCF format outputs

Specifications for the Ion Proton Sequencer

Working environment (for indoor use only)	Temperature: 68-77° F (20-25° C) Humidity: 40-60%, noncondensing Altitude: <6,500 ft (2,000 m) Clearances: 12 in (30.5 cm) in rear 4 in (10 cm) on left side 4 in (10cm) on right side 4 in (10 cm) from front edge of bench to sequencer bezel 36 in (90 cm) aisle in front of bench for operator access Optional rack mounting with two Ion Proton Sequencers per rack^†
Gas Supply	Connection: 0.25 in push-to-connect fitting Pressure: 30 psi Composition: nitrogen (grade 4.8, 99.998% or better)
Other connections	Ethernet: 1 GigE USB: 2x USB 2.0
Power	Voltage: 100 V (min) to 240 V (max) Current: 14 A (max) Frequencing: 50/60 Hz Power Draw: 1,350 W
Dimensions	Width: 21.3 in/54.2 cm Depth: 30.5 in/77.5 cm Height: 18.7in/47.4 cm
Weight	Crated for shipment: 200 lb/90.7 kg Free-standing: 130 lb/59 kg

†Space-saving rack can be purchased for the Ion Proton System. Contact your sales representative for details.

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Service and support

Our service plans help keep you up and running. With service and support that spans the sequencing workflow from sample prep through validation, our service plans give you peace of mind wherever your research takes you.

Professional installation
Premium training package conducted by field scientists
Worldwide application, technical, and field-training support

Features	AB Complete	AB Assurance	AB Maintenance
Repair response time (days)	Guaranteed next day on-site repairs‡	Guaranteed 2-day on-site repairs‡	Target 2-day remedial repairs‡
Remote instrument monitoring and diagnostics	✓	✓
Priority phone and email access to instrument support	✓	✓	✓
Instrument operation software upgrades	✓
Instrument qualification services	✓
Planned maintenance	✓	✓	✓
Parts, labor and travel	Included	Included	Discount with Plus plan