Key applications
- ChIP sequencing
- Exome sequencing
- Gene expression sequencing
- De novo sequencing
- Small RNA sequencing
- Whole transcriptome sequencing
Areas of interest
- Agricultural research
- Stem cell research
- Cancer research
- Epigenomics
- Ancient DNA genomics
- Metagenomics
Chip compatibility
Ion PI v3 Chip
60–80M reads
~2.5 hours run time
Ion Proton System performance specifications with Ion PI Chip
| Throughput | Up to 15 Gb |
| Read length | Up to 200-base fragment reads |
| Number of reads passing filter | 60-80 million reads passing filter |
| Sequencing run time | 2.5 hours |
| Barcoding solutions | 384 barcodes supported by Torrent Suite Software 96 off-the-shelf barcodes for DNA 16 off-the-shelf barcodes for RNA |
| Software solutions | Torrent Suite Software utilizing Torrent Browser including TMAP alignment and Torrent Variant Caller for germline or somatic mutation detection. Torrent Browser (included) offers users remote web access to instrument status monitoring, run quality reports, individual data files, and extensible plug-ins for application-specific analysis. Plugins available for download from the Torrent Browser Plugin Store for analysis of genome sequencing, targeted sequencing, variant analysis and annotation, microbial sequencing, transfer to 3rd party bioinformatics packages, and more. Data can optionally flow into Ion Reporter Software for controlled analysis, annotation, and reporting of variants. Ion Reporter Software provides application specific workflows to support single, trio and paired tumor normal analysis. |
| Data formats | Industry standard FASTQ, SFF, BAM and VCF format outputs |
Specifications for the Ion Proton Sequencer
| Working environment (for indoor use only) | Temperature: 68-77° F (20-25° C) Humidity: 40-60%, noncondensing Altitude: <6,500 ft (2,000 m) Clearances:
12 in (30.5 cm) in rear
Optional rack mounting with two Ion Proton Sequencers per rack†4 in (10 cm) on left side 4 in (10cm) on right side 4 in (10 cm) from front edge of bench to sequencer bezel 36 in (90 cm) aisle in front of bench for operator access |
| Gas Supply | Connection: 0.25 in push-to-connect fitting Pressure: 30 psi Composition: nitrogen (grade 4.8, 99.998% or better) |
| Other connections | Ethernet: 1 GigE USB: 2x USB 2.0 |
| Power | Voltage: 100 V (min) to 240 V (max) Current: 14 A (max) Frequencing: 50/60 Hz Power Draw: 1,350 W |
| Dimensions | Width: 21.3 in/54.2 cm Depth: 30.5 in/77.5 cm Height: 18.7in/47.4 cm |
| Weight | Crated for shipment: 200 lb/90.7 kg Free-standing: 130 lb/59 kg |
†Space-saving rack can be purchased for the Ion Proton System. Contact your sales representative for details.
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Service and support
Our service plans help keep you up and running. With service and support that spans the sequencing workflow from sample prep through validation, our service plans give you peace of mind wherever your research takes you.
- Professional installation
- Premium training package conducted by field scientists
- Worldwide application, technical, and field-training support
| Features | AB Complete | AB Assurance | AB Maintenance |
|---|---|---|---|
| Repair response time (days) | Guaranteed next day on-site repairs‡ | Guaranteed 2-day on-site repairs‡ | Target 2-day remedial repairs‡ |
| Remote instrument monitoring and diagnostics | ✓ |
✓ |
|
| Priority phone and email access to instrument support | ✓ |
✓ |
✓ |
| Instrument operation software upgrades | ✓ |
||
| Instrument qualification services | ✓ |
||
| Planned maintenance | ✓ |
✓ |
✓ |
| Parts, labor and travel | Included | Included | Discount with Plus plan |
‡Availability limited in some geographic areas
We also offer bioinformatics services to help you harness the power of Ion Torrent technology.
Not finding what you're looking for?
Resources
Support
- Technical support
- Next-Generation Sequencing Support Center—Find tips, troubleshooting help, and resources for your next-gen sequencing applications.
For Research Use Only. Not for use in diagnostic procedures.
