Pluripotent Stem Cell (PSC) Culture Media

PSC research requires careful attention to culture conditions. From KnockOut media to Essential 8 media and cell therapy research media, Gibco media deliver culture with confidence. Find the right solution for your stem cell culture needs.

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Gibco PSC Media are manufactured according to cGMP for medical devices, 21 CFR Part 820


Which pluripotent stem cell culture medium is right for you?

 

Product

KnockOut Serum Replacement – MultiSpecies

CTS KnockOut SR XenoFree Medium

StemFlex Medium

Essential 8 Medium

CTS Essential 8 Medium

Ideal for

Feeder-based human and mouse PSC culture, reprogramming, gene editing, and differentiation

Translational or clinical research applications

Robust maintenance of PSC culture, difficult cell lines, and superior performance in single cell passaging and gene editing

Consistent PSC culture and superior reprogramming

Translational or clinical research applications

Defined

Animal origin components (BSA)

Xeno-free; no animal derived components, human derived

Animal origin components
(BSA)

Xeno-free; no animal derived components, human derived

Animal origin free (AOF)

Recommended cell types

Mammalian PSCs

Human PSCs

Human PSCs

Human PSCs Human PSCs

Weekend-free feeding schedule

No

No

Yes

Yes
Essential 8 Flex Medium

No

Genome editing

Fair

Fair

Best

Fair

Fair

Robustness

Good

Good

Best

Fair

Fair
Recommended
matrix
Gibco Mouse Embryonic Fibroblasts and Attachment Factor (for human and mouse) Geltrex Matrix

Geltrex Matrix
rhLaminin-521

Vitronectin (VTN-N) Recombinant Protein
rhLaminin-521

CTS Vitronectin (VTN-N) Recombinant Protein
rhLaminin-521

Recommended level of dissociation and passaging reagent

Clump

Gibco Collagenase IV

Versene Solution

Versene Solution

Versene Solution

Versene Solution

Small cluster

N/A

N/A

StemPro Accutase Cell Dissociation Reagent, 
RevitaCell Supplement helpful but not required

StemPro Accutase Cell Dissociation Reagent, optional RevitaCell Supplement added to medium during first 18-24 hours post-passage for improved recovery

StemPro Accutase Cell Dissociation Reagent, optional RevitaCell Supplement added to medium during first 18-24
hours post-passage for improved recovery

Single cell

TrypLE Select  Enzyme (for mouse)

Gibco CTS TrypLE Select Enzyme**

TrypLE Select or Tryple Express Enzyme, RevitaCell Supplement helpful but not required during recovery if using rhLaminin-521

TrypLE Select or Tryple Express Enzyme with RevitaCell Supplement added to medium during first 18-24 hours post-passage

CTS TrypLE Select Enzyme with RevitaCell Supplement added to medium during first 18-24 hours post-passage

Unless otherwise indicated, all products are For Research Use Only. Not for use in diagnostic procedures
*For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal Law restricts this device to sale by or on the order of a physician.
**For Research Use or Noncommercial Manufacturing of Cell-Based Products for Clinical Research. CAUTION: Not for direct administration into humans or animals.