Pluripotent stem cell passaging or dissociating can be accomplished using various methods depending on your culture medium and application. Whether you prefer to passage your cell enzymatically or manually, Gibco™ media has the solution for your PSC subculture.
Passaging Pluripotent Stem Cells
Which passaging method is right for you?
|Recommended for clump passaging in feeder and feeder-free cultures||Standard reagent for clump passaging in feeder and feeder-free cultures||Quick and gentle dissociation, recommended for single cell passaging||Recommended for clump passaging with feeder-free Essential 8™ Medium||Optimal for clinical research applications that require human origin materials only||Quick and easy manual passaging tool for feeder and feeder-free cultures|
|Product name||Dispase||Collagenase IV||Accutase||UltraPure™ 0.5M EDTA||TrypLE ™ Select CTS™||StemPro™ EZPassage™ Disposable Stem Cell Passaging Tool|
|Recommended applications||Passaging in clumps||Passaging in clumps||Dissociation to single cells||Passaging in clumps||Passaging in clumps or single cell passaging||Mechanical passaging in clumps|
|Source origin||Bacillus polymyxa||Clostridium histolyticum||Marine||Chemical animal-origin free||Recombinant enzyme animal-origin free||Not applicable|
|Form||Lyophilized powder||Lyophilized powder||Liquid, ready to use||Liquid, dilute 1,000x||Liquid, ready to use||Individually wrapped|
|Storage temperature||2-8°C||2-8°C||2-8°C||Room temperature||Room temperature||Room temperature|
|Shelf life of liquid format||2 weeks||2 weeks||2 years||2 years||2 years||Not applicable|
|Inactivation method||Inhibition by dilution (no inhibitors needed)||Inhibition by dilution (no inhibitors needed)||Inhibition by dilution (no inhibitors needed)||Inhibition by dilution (no inhibitors needed)||Inhibition by dilution (no inhibitors needed)||Not applicable|
|Unit size||5 g||1 g||100 mL||4 x 100 mL||100 mL||Box of 10 units|
|Optimized concentration for use||2 mg/mL||1 mg/mL for feeder-dependent, 10 mg/mL for feeder-free||1X Solution||0.5 mM||1X Solution||Not applicable|
|Number of plates passaged/unit||1,250 x 60-mm plates||500 x 60-mm plates at 1 mg/mL, 50 x 60-mm plates at 10 mg/mL||50 x 60-mm plates||50,000 x 60-mm plates||50 x 60-mm plates||10 plates|