Pluripotent stem cell passaging or dissociating can be accomplished using various methods depending on your culture medium and application. Whether you prefer to passage your cell enzymatically or manually, Gibco™ media has the solution for your PSC subculture.

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Which passaging method is right for you?

Recommended for clump passaging in feeder and feeder-free cultures Standard reagent for clump passaging in feeder and feeder-free cultures Quick and gentle dissociation, recommended for single cell passaging Recommended for clump passaging with feeder-free Essential 8™ Medium Optimal for clinical research applications that require human origin materials only Quick and easy manual passaging tool for feeder and feeder-free cultures
Order Order Order Order Order Order
Product name Dispase Collagenase IV Accutase UltraPure™ 0.5M EDTA TrypLE ™ Select CTS™ StemPro™ EZPassage™ Disposable Stem Cell Passaging Tool
Recommended applications Passaging in clumps Passaging in clumps Dissociation to single cells Passaging in clumps Passaging in clumps or single cell passaging Mechanical passaging in clumps
Source origin Bacillus polymyxa Clostridium histolyticum Marine Chemical animal-origin free Recombinant enzyme animal-origin free Not applicable
Form Lyophilized powder Lyophilized powder Liquid, ready to use Liquid, dilute 1,000x Liquid, ready to use Individually wrapped
Storage temperature 2-8°C 2-8°C 2-8°C Room temperature Room temperature Room temperature
Shelf life of liquid format 2 weeks 2 weeks 2 years 2 years 2 years Not applicable
Inactivation method Inhibition by dilution (no inhibitors needed) Inhibition by dilution (no inhibitors needed) Inhibition by dilution (no inhibitors needed) Inhibition by dilution (no inhibitors needed) Inhibition by dilution (no inhibitors needed) Not applicable
Unit size 5 g 1 g 100 mL 4 x 100 mL 100 mL Box of 10 units
Optimized concentration for use 2 mg/mL 1 mg/mL for feeder-dependent, 10 mg/mL for feeder-free 1X Solution 0.5 mM 1X Solution Not applicable
Number of plates passaged/unit 1,250 x 60-mm plates 500 x 60-mm plates at 1 mg/mL, 50 x 60-mm plates at 10 mg/mL 50 x 60-mm plates 50,000 x 60-mm plates 50 x 60-mm plates 10 plates