Phase-contrast image shows an overlay of TRA-1-81 staining (red) of a feeder-free H9 ESC colony.

Whether you are culturing your cells on feeder layers or require feeder-free, xeno-free conditions for more clinical applications, Gibco products provide you with all the choices to culture your pluripotent stem cells in the right environment. W e provide extracellular matrices (ECMs) that are designed to:

  • Minimize adaptation time,
  • Maximize cell performance, and
  • Help meet regulatory requirements

Which extracellular matrix is right for you?

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 Most commonly used basement membrane matrix for ESC and iPSC culturesSimplified solution for the most commonly used basement membraneOptimized for use with Essential 8 MediumSuperior recovery during challenging transitions
Product name Geltrex LDEV-Free hESC Qualified Geltrex hESC Qualified, Ready-To-UseVitronectin (VTN-N)Laminin 521
CTS version availableNot currently availableCTS CELLstart SubstrateCTS Vitronectin (VTN-N) Recombinant Human ProteinNot currently available

Recommended applications Feeder and feeder-free cultureFeeder and feeder-free cultureFeeder-free and xeno-free cultureFeeder-free and xeno-free culture
Recommended medium pairing StemPro hESC SFM or StemFlex MediumStemPro hESC SFM or StemFlex MediumStemFlex Medium or Essential 8 Flex MediumStemFlex Medium or Essential 8 Flex Medium
Source originCultured mouse EHS tumor cells; undefinedCultured mouse EHS tumor cells; undefinedHuman (recombinant); definedHuman (recombinant); defined
Storage temperatureFrozen (-20 to -80° C)Refrigerate (2-8° C)Frozen (-20 to -80° C)Frozen (-20 to -80° C)
Reagent preparation timeThaw on ice, overnight in refrigerator, dilute 1:100Ready to useThaw at room temperature, dilute 1:100Thaw at 2-8° C, dilute 1:50
Unit size1 mL or 5 mL50 mL1 mL1 mL or 10 mL
Number of plates coated/unit/span16x6 well plate from 1 mL vial diluted 1:1008x6 well plates16x6 well plates from 1 mL vial diluted 1:1003 x 6 well plate from 1 mL

Attachment Factor 0.1% Gelatin, (Cat. No. S006100) may be used for feeder-layer attachment.

Intended use of the products mentioned on this page vary. For specific intended use statements please refer to the product label.