Extracellular Matrices for PSC Culture

We provide extracellular matrices (ECMs) that are designed to minimize adaptation time, maximize cell performance, and help meet regulatory requirements.

Whether you are culturing your cells on feeder layers or require feeder-free xeno-free conditions for more clinical applications, Gibco® products provide you with all the choices to culture your pluripotent stem cells (PSC) in the right environment.

Figure 1 (left): H9 cells grown on Geltrex® hESC-qualified Ready-To-Use Matrix and Essential™ 8 Medium, stained for Tra-1-81 expression.

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Which extracellular matrix is right for you?

Most commonly used basement membrane matrix for ESC and iPSC cultures Simplified solution for the most commonly used basement membrane Optimized for use with Essential 8™ Medium Superior recovery during challenging transitions
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Product name Geltrex™ LDEV-Free hESC Qualified Geltrex® hESC Qualified, Ready-To-Use Vitronectin (VTN-N) Laminin 521
Recommended applications Feeder and feeder-free culture Feeder and feeder-free culture Feeder-free and xeno-free culture Feeder-free and xeno-free culture
Recommended medium pairing StemPro® hESC SFM or Essential 8™ Medium StemPro® hESC SFM or Essential 8™ Medium Essential 8™ Medium Essential 8™ Medium
Source origin Cultured mouse EHS tumor cells; undefined Cultured mouse EHS tumor cells; undefined Human (recombinant); defined Human (recombinant); defined
Storage temperature Frozen (-20 to -80° C) Refrigerate (2-8° C) Frozen (-20 to -80° C) Frozen (-20 to -80° C)
Reagent preparation time Thaw on ice, overnight in refrigerator, dilute 1:100 Ready to use Thaw at room temperature, dilute 1:100 Thaw at 2-8° C, dilute 1:50
Unit size 1 mL or 5 mL 50 mL 1 mL 1 mL or 10 mL
Number of plates coated/unit/span> 16x6 well plate from 1 mL vial diluted 1:100 8x6 well plates 16x6 well plates from 1 mL vial diluted 1:100 3 x 6 well plate from 1 mL

Attachment Factor (0.1% Gelatin, Cat. No. S006100) may be used for feeder-layer attachment.