Whether you are culturing your cells on feeder layers or require feeder-free, xeno-free conditions for more clinical applications, Gibco products provide you with all the choices to culture your pluripotent stem cells in the right environment. W e provide extracellular matrices (ECMs) that are designed to:
|Order now||Order now||Order now||Order now|
|Most commonly used basement membrane matrix for ESC and iPSC cultures||Simplified solution for the most commonly used basement membrane||Optimized for use with Essential 8 Medium||Superior recovery during challenging transitions|
|Product name||Geltrex LDEV-Free hESC Qualified||Geltrex hESC Qualified, Ready-To-Use||Vitronectin (VTN-N)||Laminin 521|
|CTS version available||Not currently available||CTS CELLstart Substrate||CTS Vitronectin (VTN-N) Recombinant Human Protein||Not currently available|
|Recommended applications||Feeder and feeder-free culture||Feeder and feeder-free culture||Feeder-free and xeno-free culture||Feeder-free and xeno-free culture|
|Recommended medium pairing||StemPro hESC SFM or StemFlex Medium||StemPro hESC SFM or StemFlex Medium||StemFlex Medium or Essential 8 Flex Medium||StemFlex Medium or Essential 8 Flex Medium|
|Source origin||Cultured mouse EHS tumor cells; undefined||Cultured mouse EHS tumor cells; undefined||Human (recombinant); defined||Human (recombinant); defined|
|Storage temperature||Frozen (-20 to -80° C)||Refrigerate (2-8° C)||Frozen (-20 to -80° C)||Frozen (-20 to -80° C)|
|Reagent preparation time||Thaw on ice, overnight in refrigerator, dilute 1:100||Ready to use||Thaw at room temperature, dilute 1:100||Thaw at 2-8° C, dilute 1:50|
|Unit size||1 mL or 5 mL||50 mL||1 mL||1 mL or 10 mL|
|Number of plates coated/unit/span||16x6 well plate from 1 mL vial diluted 1:100||8x6 well plates||16x6 well plates from 1 mL vial diluted 1:100||3 x 6 well plate from 1 mL|
Attachment Factor 0.1% Gelatin, (Cat. No. S006100) may be used for feeder-layer attachment.
Intended use of the products mentioned on this page vary. For specific intended use statements please refer to the product label.