Extracellular Matrices for PSC Culture
We provide extracellular matrices (ECMs) that are designed to minimize adaptation time, maximize cell performance, and help meet regulatory requirements.
Whether you are culturing your cells on feeder layers or require feeder-free xeno-free conditions for more clinical applications, Gibco® products provide you with all the choices to culture your pluripotent stem cells (PSC) in the right environment.
Figure 1 (left): H9 cells grown on Geltrex® hESC-qualified Ready-To-Use Matrix and Essential™ 8 Medium, stained for Tra-1-81 expression.
Which extracellular matrix is right for you?
|Most commonly used basement membrane matrix for ESC and iPSC cultures||Simplified solution for the most commonly used basement membrane||Optimized for use with Essential 8™ Medium||Superior recovery during challenging transitions|
|Order now||Order now||Order now||Order now|
|Product name||Geltrex™ LDEV-Free hESC Qualified||Geltrex® hESC Qualified, Ready-To-Use||Vitronectin (VTN-N)||Laminin 521|
|Recommended applications||Feeder and feeder-free culture||Feeder and feeder-free culture||Feeder-free and xeno-free culture||Feeder-free and xeno-free culture|
|Recommended medium pairing||StemPro® hESC SFM or Essential 8™ Medium||StemPro® hESC SFM or Essential 8™ Medium||Essential 8™ Medium||Essential 8™ Medium|
|Source origin||Cultured mouse EHS tumor cells; undefined||Cultured mouse EHS tumor cells; undefined||Human (recombinant); defined||Human (recombinant); defined|
|Storage temperature||Frozen (-20 to -80° C)||Refrigerate (2-8° C)||Frozen (-20 to -80° C)||Frozen (-20 to -80° C)|
|Reagent preparation time||Thaw on ice, overnight in refrigerator, dilute 1:100||Ready to use||Thaw at room temperature, dilute 1:100||Thaw at 2-8° C, dilute 1:50|
|Unit size||1 mL or 5 mL||50 mL||1 mL||1 mL or 10 mL|
|Number of plates coated/unit/span>||16x6 well plate from 1 mL vial diluted 1:100||8x6 well plates||16x6 well plates from 1 mL vial diluted 1:100||3 x 6 well plate from 1 mL|
Attachment Factor (0.1% Gelatin, Cat. No. S006100) may be used for feeder-layer attachment.