5 steps to model cardiac disease

iPSCs and cardiac disease modeling

With the increasing need for in vitro human disease models, induced pluripotent stem cells (iPSCs) have been identified as showing remarkable potential to serve this purpose. In conjunction with CRISPR-Cas9 genome editing tools, human iPSCs have given researchers the ability to identify disease-genes, model human diseases, and execute regenerative therapy research strategies in vitro.

Modeling cardiac disease

Cardiovascular diseases, including coronary heart disease, rheumatic heart disease, congenital heart disease, are the leading cause of deaths globally, affecting nearly 20 million people each year. Cardiovascular disease is an umbrella term used to describe a variety of conditions in which the blood vessels are obstructed in a way that leads to acute events such as heart attack and stroke, or conditions in which cardiac morphology or function is affected. The further study of these diseases requires the ability to obtain human diseased tissue for study and a high throughput method to test a variety of drugs. Now, with the use of human induced pluripotent stem cells (hiPSCs), it is much more feasible to culture and control cardiovascular disease fibroblasts and differentiate them into contracting cardiomyocytes. Review the 5 Steps to Model Cardiac Disease guide below and see how our scientists created a Dilated Cardiomyopathy (DCM)-associated mutation of the cardiac muscles troponin T gene (TNNT2 R141Q) in hiPSCs.

Follow our 5 steps application guide to help improve your success in modeling cardiac diseases

Free download: Disease model generation: 5 steps to model cardiac diseases

Disease model generation: 5 steps to model cardiac diseases guide

Overcome the challenges of genome editing in human pluripotent stem cells for building tissue-relevant disease models. Use this proven guide and validate steps to facilitate generation of your iPSC-derived cardiac disease model.

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Find an overview of our 5 steps application guide below to help improve your success in modeling cardiac disease

Diagram showing 5 steps of neurodegenerative disease modeling from genome editing to cell assays

Step 1: Edit

Pluripotent Stem Cell Gene Editing Demo Kit

Explore our comprehensive catalog of next-generation editing tools designed to help improve gene editing efficiency in human pluripotent stem cells to enhance your human disease modeling studies.

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Step 2: Clone

StemFlex Medium

Isolate and expand the clones from a pool of gene-editing hiPSCs. Consider our state-of-the-art hiPSC culture reagents that are optimized for reliable single-cell sorting and maximum clonal recovery.

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Step 3: Characterize

Efficiently check pluripotency and genomic stability of stem cells and their microenvironments to assure the accuracy of your disease model. Try our complete solutions for qualification of human pluripotent stem cell (hPSC) line.

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Learn more

Step 4: Differentiate

PSC Cardiomyocyte Differentiation Kit

Harness the power of our high-quality growth factors and cytokines for targeted differentiation of stem cells, manufactured to help ensure high biological activity, high purity, freeze-thaw stability, and structural homogeneity.

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Step 5: Measure

FluoVolt Membrane Potential Kit

Extract the information you need from your models using the entire fluorescence spectrum to optimize your assay and predict what assays are coming next.

Product highlight

Other recommended products

Step 1: Edit

Pluripotent Stem Cell Gene Editing Demo Kit

Explore our comprehensive catalog of next-generation editing tools designed to help improve gene editing efficiency in human pluripotent stem cells to enhance your human disease modeling studies.

Product highlight

Other recommended products

Step 2: Clone

StemFlex Medium

Isolate and expand the clones from a pool of gene-editing hiPSCs. Consider our state-of-the-art hiPSC culture reagents that are optimized for reliable single-cell sorting and maximum clonal recovery.

Product highlight

Other recommended products

Step 3: Characterize

Efficiently check pluripotency and genomic stability of stem cells and their microenvironments to assure the accuracy of your disease model. Try our complete solutions for qualification of human pluripotent stem cell (hPSC) line.

Services highlight

Other recommended products

Learn more

Step 4: Differentiate

PSC Cardiomyocyte Differentiation Kit

Harness the power of our high-quality growth factors and cytokines for targeted differentiation of stem cells, manufactured to help ensure high biological activity, high purity, freeze-thaw stability, and structural homogeneity.

Product highlight

Other recommended products

Step 5: Measure

FluoVolt Membrane Potential Kit

Extract the information you need from your models using the entire fluorescence spectrum to optimize your assay and predict what assays are coming next.

Product highlight

Other recommended products