scorecard

Quantitative analysis of trilineage differentiation potential

The Applied Biosystems™ TaqMan® hPSC Scorecard™ Panel assesses pluripotency and trilineage differentiation potential using real-time qPCR assays and intuitive data analysis software.

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Quantify trilineage differentiation potential of your PSCs

Why We Chose the TaqMan hPSC Scorecard Assay
Jennifer Moore, RUCDR/Infinite Biologics
 

The hPSC Scorecard assay was developed in collaboration with Alex Meissner, and follows from his landmark publication (Cell 2011 144:439–452). The accompanying analysis software gives researchers anywhere in the world the ability to compare the gene expression profiles of their cell lines to a common reference set.

Instrument requirements

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See which instruments are compatible with the TaqMan hPSC Scorecard Panel and how to set them up.

Data analysis

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The TaqMan hPSC Scorecard Panel includes access to free, intuitive web-based analysis software that allows researchers to quantitatively compare the gene expression profile of their sample to that of a reference set.

CellModel Services

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What is it?

The TaqMan hPSC Scorecard Panels quantitatively confirms pluripotency (trilineage differentiation potential)
for both embryonic and induced pluripotent stem cell lines. The hPSC Scorecard™ Panel (available in 96 and 384-well format) contains 94 predefined TaqMan Gene Expression assays (including endogenous controls) dried-down in assay plate wells. The panel of genes was developed in collaboration with Alex Meissner, and based on his landmark publication (Cell 144:439-452 (2011)).


The TaqMan hPSC Scorecard Panel provides:

  • A quantitative assay for confirming pluripotency and trilineage differentiation potential
  • Comparison of expression profiles to a reference standard
  • An easy to use platform with pre-plated assays and dedicated, intuitive analysis software


Overview of protocol:

  1. Test undifferentiated, spontantious differentiation (EB) or directed (monolayer) differentiation samples
  2. Extract and purify RNA
  3. Generate cDNA
  4. Run gene expression panel on a compatible qRT-PCR instrument 
  5. Upload .eds files to the hPSC Scorecard Analysis Software
  6. Analyze data and export results from the convenient cloud-based analysis application
     

How to run the hPSC Scorecard Assay

Undifferentiated ESC/iPSC

Differentiated ESC/iPSC: Embryoid bodies (week 1) - or - Directly differentiated cells.

Sample requirements: >1 x 106 cells/sample

RNA Isolation

 

 Formulas

TRIzol® organic phase extraction

Sample requirements: 1 mL TRIzol® reagent per 6 cm culture dish

[RNA] (mg/mL) = A260 * 40

Recommended A260/A280 ratio = 1.6 – 1.8

Analyze data from the TaqMan® hPSC Scorecard™ Panel using the intuitive analysis software

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