TaqMan hPSC Scorecard Assay

Quantitative analysis of trilineage potential of your PSCs

The Applied Biosystems TaqMan hPSC Scorecard Panel assesses pluripotency and trilineage differentiation potential using real-time qPCR assays and intuitive data analysis software. The hPSC Scorecard assay was developed in collaboration with Alex Meissner, and follows from his landmark publication (Cell 2011 144:439–452). The accompanying analysis software gives researchers anywhere in the world the ability to compare the gene expression profiles of their cell lines to a common reference set.

Need help?  View protocol

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Why we chose the TaqMan hPSC Scorecard Assay

Hear from Jennifer Moore at RUCDR/Infinite Biologics on the benefits Scorecard has to offer.

Instrument requirements

Instrument requirements

See which instruments are compatible with the TaqMan hPSC Scorecard Panel and how to set them up.

Data analysis

Data analysis

The TaqMan hPSC Scorecard Panel includes access to free, intuitive web-based analysis software that allows researchers to quantitatively compare the gene expression profile of their sample to that of a reference set.

CellModel Services

CellModel Services

Lacking the resources or instruments to use the Scorecard Panel? Let us do it for you!

What is it?

The TaqMan hPSC Scorecard Panels quantitatively confirms pluripotency (trilineage differentiation potential)
for both embryonic and induced pluripotent stem cell lines. The hPSC Scorecard Panel (available in 96 and 384-well format) contains 94 predefined TaqMan Gene Expression assays (including endogenous controls) dried-down in assay plate wells. The panel of genes was developed in collaboration with Alex Meissner, and based on his landmark publication (Cell 144:439-452 (2011)).


The TaqMan hPSC Scorecard Panel provides:

  • A quantitative assay for confirming pluripotency and trilineage differentiation potential
  • Comparison of expression profiles to a reference standard
  • An easy to use platform with pre-plated assays and dedicated, intuitive analysis software


Overview of protocol:

  1. Test undifferentiated, spontantious differentiation (EB) or directed (monolayer) differentiation samples
  2. Extract and purify RNA
  3. Generate cDNA
  4. Run gene expression panel on a compatible qRT-PCR instrument 
  5. Upload .eds files to the hPSC Scorecard Analysis Software
  6. Analyze data and export results from the convenient cloud-based analysis application
     

How to run the hPSC Scorecard Assay

Undifferentiated ESC/iPSC

Differentiated ESC/iPSC: Embryoid bodies (week 1) - or - Directly differentiated cells.

Sample requirements: >1 x 106 cells/sample

Undifferentiated ESC/iPSC

Feeder-dependent culture

Feeder-free culture

Differentiated ESC/iPSC (EBs)

RNA Isolation

Formulas

TRIzol organic phase extraction

Sample requirements: 1 mL TRIzol reagent per 6 cm culture dish

[RNA] (mg/mL) = A260 * 40

Recommended A260/A280 ratio = 1.6 – 1.8

8 reverse transcription (RT) reactions per RNA sample

cDNA Preparation

Download run template

Experiment type: Standard Curve

384-well configuration

96-well configuration

Analyze data from the TaqMan hPSC Scorecard Panel using the intuitive analysis software

Sign in to the hPSC Scorecard Analysis Software

Step 1 : Cell Culture

Undifferentiated ESC/iPSC

Differentiated ESC/iPSC: Embryoid bodies (week 1) - or - Directly differentiated cells.

Sample requirements: >1 x 106 cells/sample

Undifferentiated ESC/iPSC

Feeder-dependent culture

Feeder-free culture

Differentiated ESC/iPSC (EBs)

Step 2 : RNA preparation

RNA Isolation

Formulas

TRIzol organic phase extraction

Sample requirements: 1 mL TRIzol reagent per 6 cm culture dish

[RNA] (mg/mL) = A260 * 40

Recommended A260/A280 ratio = 1.6 – 1.8

Step 3 : cDNA preparation

8 reverse transcription (RT) reactions per RNA sample

cDNA Preparation

Step 4 : qRT-PCR

Download run template

Experiment type: Standard Curve

384-well configuration

96-well configuration

Step 5 : Data analysis

Analyze data from the TaqMan hPSC Scorecard Panel using the intuitive analysis software

Sign in to the hPSC Scorecard Analysis Software

For Research Use Only. Not for use in diagnostic procedures.