Stem Cell Engineering

Manipulating cells for downstream applications

Stm cell engineering requires introduction of new genetic content into a cell. One specific type of stem cell engineering is reprogramming by conversion of somatic cells into induced pluripotent stem cells. This can be accomplished with Thermo Fisher’s non-integrating technologies by viral delivery or episomal plasmids. Once cell lines have been established, genomic editing and manipulation of iPSCs into other cell types allows for the building of cell models to better answer biological questions.

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Reprogramming

CytoTune-iPS 2.0 Sendai Reprogramming Kit
Highest-efficiency, integration-free system

Epi5 Episomal iPSC Reprogramming Vectors
High efficiency viral-free system

Genome editing

CRISPR Products and Services
Consistently high editing efficiency across all gene targets and cell types.

TALEN-Based Genome Editing
Precise and flexible editing; targeting to any gene in any cell, with all-around freedom.

StemFlex Medium
Supports up to 2-fold faster recovery following gene editing

Services

CellModel Services
Reprogrammed cells provided in as little as 4 to 6 months

GeneArt Genes-to-Cell Lines Services
Allow us to generate high-performance cell lines with stable expression of your gene

Stem cell reprogramming

Reprogramming somatic cells to induced pluripotent stem cells is a critical and potentially time-intensive step in stem cell research. We offer choices in integration-free stem cell reprogramming technologies and services to support your research and translational needs including our Cell Therapy Systems (CTS) products.

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Genome editing

From precision genome editing and gene modification technologies to high-efficiency delivery systems, we have developed a complete toolset comprising trusted solutions to help you create the modified genes, expression systems and stable cell lines you need for your research—from culturing cells to modification, then detection and analysis. Whether you want results fast, seek full control over every step in designing your gene edit, or need help with engineering cells to your specific needs, we have solutions that fit.

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Additional stem cell engineering tools

In addition to innovative technologies and services for reprogramming and genomic editing of stem cells, we offer tools for cell transfection, transduction, and cloning.

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  • Neon Transfection System—Electroporation technology for highly efficient delivery of nucleic acids into virtually any animal cell type. The system can be used for reprogramming somatic cells into iPSCs.
  • Gateway cloning—Circumvent traditional restriction enzyme–based cloning limitations; Gateway cloning enables access to virtually any expression system.
  • BacMam technology—A baculovirus delivery system for efficient transduction of mammalian cell lines, including primary and stem cells.
  • Lipofectamine 3000—Advanced lipid nanoparticle technology for superior transfection efficiency in a broad spectrum of difficult-to-transfect cell types. This reagent kit can be used for generation and transfection of iPSCs.