The TaqMan® hPSC Scorecard™ Panel enables verification of pluripotency and determination of lineage bias for both embryonic and induced pluripotent stem cell lines. For stem cell researchers seeking to access the characterization data that the Scorecard Panel provides, but who lack the resources or instrumentation to use the panel, our CellModel™ Services offer a convenient alternative to obtain the same data.

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The TaqMan hPSC Scorecard Panel provides:

  • Confidence in your data through validated, focused gene content
  • An easy-to-use platform for assessing pluripotency and trilineage bias in the same experiment
  • Comparison of results to a reference standard and across multiple experiments
     

 

 

TaqMan® hPSC Scorecard™ Panel software produces an easy-to-read data output to indicate pluripotency and lineage bias.

TaqMan® hPSC Scorecard Panel characterization projects can typically be completed in 1 to 2 weeks; depending on the starting material you provide (more time if embryoid body (EB) formation is required).

Sample Type Required amount and method of prep Timeline for report
Purified RNA 10–20 µg in nuclease-free water or buffer 1–2 weeks
iPSC, EBs or differentiated cells 2 vials of 5x106 cells in TRIzol™ 1–2 weeks
Undifferentiated cells that require EB formation 2 vials of 1x106 cells 4–6 weeks
Need to obtain iPS cells first? Our stem cell reprogramming services are available to generate iPS cells from a human sample.

Example data: Time course of embryoid body formation

View a full sample data report

This TaqMan® hPSC Scorecard™ Panel results summary demonstrates the pluripotency and lineage bias data produced by the panel software.  In this example, the panel shows expected trends in gene expression.

TaqMan hPSC Scorecard Panel

Scores are a statistical comparison of the expression profile of the sample to that of the undifferentiated reference set.


Colors correlate to the fold change in expression of the indicated gene relative to the undifferentiated reference set.

CellModel™ Services enable stem cell scientists to reach their desired outcomes faster.  Customize your project with additional upstream and downstream steps like these below.

  • Embryoid body formation for Taqman® hPSC Scorecard assay  
  • iPS cell reprogramming prior to the Scorecard assay
  • Differentiation to neural stem cells or terminal neural lineages

Start your stem cell characterization project today!

Contact us today to discuss available services using the TaqMan® hPSC Scorecard™ Panel.

For Research Use Only. Not for use in diagnostic procedures.