Thank you for your interest in Molecular Probes® products and educational resources. The Molecular Probes® HandbookA Guide to Fluorescent Probes and Labeling Technologies is a comprehensive scientific guide to over 3,000 reagents and kits representing a diverse collection of Molecular Probes® labeling and detection products.

Download a chapter from the 11th Edition, printed in 2010, from the table below, or browse the most up-to-date online version using the navigation in the left column. Be sure to bookmark this page so that you can easily return to download more chapters.

The Molecular Probes® Handbook—PDF downloads by chapter

Chapter Title
 Introduction to Fluorescence Fundamentals (930 KB)
Part I—Choosing a Fluorescent Label: Their Properties and Labeling Chemistries
 Chapter 1—Fluorophores and Their Amine-Reactive Derivatives (7.2 MB)
 Chapter 2—Thiol-Reactive Probes (2.5 MB)
 Chapter 3—Click Chemistry and Other Functional Group Modifications (1.8 MB)
 Chapter 4—Biotin and Hapten Derivatives (1.4 MB)
 Chapter 5—Crosslinking and Photoactivatable Reagents (1.6 MB)
Part II—Detecting Biomolecules: Signal Amplification and Secondary Detection
 Chapter 6—Ultrasensitive Detection Technology (2.6 MB)
 Chapter 7—Antibodies, Avidins and Lectins (2.7 MB)
Part III—Decoding Cell Components: Nucleic Acid and Protein Analysis
 Chapter 8—Nucleic Acid Detection and Analysis (3.5 MB)
 Chapter 9—Protein Detection and Analysis (2.4 MB)
 Chapter 10—Enzyme Substrates and Assays (5.4 MB)
Part IV—Probing Cell Structure: From Organelles and Membranes to Whole Cells
 Chapter 11—Probes for Cytoskeletal Proteins (1.5 MB)
 Chapter 12—Probes for Organelles (3.4 MB)
 Chapter 13—Probes for Lipids and Membranes (3.9 MB)
 Chapter 14—Fluorescent Tracers of Cell Morphology and Fluid Flow (4.3 MB)
 Chapter 15—Assays for Cell Viability, Proliferation and Function (6.5 MB)
 Chapter 16—Probes for Endocytosis, Receptors and Ion Channels (3.2 MB)
 Chapter 17—Probes for Signal Transduction (2.4 MB)
 Chapter 18—Probes for Reactive Oxygen Species, Including Nitric Oxide (2.3 MB)
 Chapter 19—Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions (6.1 MB)
 Chapter 20—pH Indicators (2.1 MB)
 Chapter 21—Indicators for Na+, K+, Cl and Miscellaneous Ions (2.6 MB)
 Chapter 22—Probes for Membrane Potential (1.6 MB)
Part VI—Integrating Technology and Instrumentation
 Chapter 23—Antifades and Other Tools for Fluorescence Applications (1.7 MB)

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