Hydrophobic Interaction Chromatography (HIC) HPLC Columns for Intact Mass Analysis

What is Hydrophobic Interaction Chromatography?

Effectively separate your intact, neutral monoclonal antibodies (mAbs) from their aggregates using hydrophobic interaction chromatography (HIC). This technique ensures high-resolution and precise separation, maintaining the integrity and functionality of your mAbs, which is essential for reliable downstream applications and therapeutic uses.

Hydrophobic interaction chromatography (HIC) HPLC columns are specifically designed to efficiently separate intact, charge-neutral monoclonal antibodies (mAbs) and Antibody Drug Conjugate (ADC) variants. This method is highly complementary to other chromatographic techniques such as ion-exchange chromatography (IEC) and size exclusion chromatography (SEC), providing an orthogonal separation option that enhances the overall analytical capabilities. The unique surface chemistry of our hydrophobic interaction columns distinguishes them from other types of columns. These columns offer excellent resolution and a broad range of selectivity, making them ideal for protein purification under non-denaturing conditions. This ensures that the structural integrity and biological activity of the proteins are preserved, which is crucial for accurate analysis and downstream applications. Our product standards and purification services ensure the highest quality and consistency in your chromatography workflows. Ideal for separating intact, charge-neutral monoclonal antibodies (mAbs) and Antibody Drug Conjugate (ADC) variants. Complements ion-exchange chromatography (IEC) and size exclusion chromatography (SEC) by offering an orthogonal separation option. Unique surface chemistry provides excellent resolution and a broad range of selectivity, preserving protein integrity under non-denaturing conditions.

Biomolecule HPLC and UHPLC columns

Recommended HIC HPLC column products for intact mass analysis

	MAbPac HIC-10 HPLC Columns	MAbPac HIC-20 HPLC columns	MAbPac HIC-Butyl HPLC columns
✓For separation of monoclonal antibodies (mAbs) and antibody-drug conjugates. ✓Unique Hydrophobic Interaction Chromatography (HIC) column chemistry; provides high resolution, excellent biocompatibility, and selectivity that ensures precise and reliable separation. ✓Offers different selectivity compared to other HIC columns available on the market, enhancing the ability to distinguish between closely related biomolecules.	MAbPac HIC-20 HPLC Columns are designed for the separation of monoclonal antibodies (mAbs), mAb fragments, and oxidized variants. ✓Employing advanced surface bonding, these columns offer excellent chemical stability, high resolution, and unique selectivity optimized specifically for mAbs. ✓Deliver distinct selectivity compared to other hydrophobic interaction chromatography (HIC) columns on the market, enhancing protein purification and analysis. ✓Ensure superior performance in separating complex biologics, meeting stringent product standards for reliable and consistent chromatography workflows. Supported by comprehensive services, these columns provide exceptional biocompatibility and efficiency in protein interaction and purification applications.	MAbPac HIC-30 HPLC Columns are specifically designed for the separation of more hydrophobic samples and antibody-drug conjugates (ADCs). ✓Featuring a hydrophilic resin, these columns provide excellent biocompatibility and low carryover, ensuring high-quality results in protein purification. ✓The optimal density of the butyl groups in the stationary phase offers high-resolution separation, making these columns ideal for complex biologics. ✓Distinct from other hydrophobic interaction chromatography (HIC) columns, they provide unique selectivity and superior performance in hydrophobic sample analysis. ✓Adhering to stringent product standards, these columns enhance the reliability and consistency of your chromatography workflows. Supported by comprehensive services, MAbPac HIC-30 columns ensure optimal performance in protein interaction and purification applications.
	Request a Quote	Request a Quote	Request a Quote

HIC HPLC Columns for Intact Mass Analysis

MAbPac HIC-10 HPLC Columns

MAbPac HIC-20 HPLC columns

MAbPac HIC-Butyl HPLC columns

✓For separation of monoclonal antibodies (mAbs) and antibody-drug conjugates.

✓Unique Hydrophobic Interaction Chromatography (HIC) column chemistry; provides high resolution, excellent biocompatibility, and selectivity that ensures precise and reliable separation.

✓Offers different selectivity compared to other HIC columns available on the market, enhancing the ability to distinguish between closely related biomolecules.

MAbPac HIC-20 HPLC Columns are designed for the separation of monoclonal antibodies (mAbs), mAb fragments, and oxidized variants.

✓Employing advanced surface bonding, these columns offer excellent chemical stability, high resolution, and unique selectivity optimized specifically for mAbs.

✓Deliver distinct selectivity compared to other hydrophobic interaction chromatography (HIC) columns on the market, enhancing protein purification and analysis.

✓Ensure superior performance in separating complex biologics, meeting stringent product standards for reliable and consistent chromatography workflows.

Supported by comprehensive services, these columns provide exceptional biocompatibility and efficiency in protein interaction and purification applications.

MAbPac HIC-30 HPLC Columns are specifically designed for the separation of more hydrophobic samples and antibody-drug conjugates (ADCs).

✓Featuring a hydrophilic resin, these columns provide excellent biocompatibility and low carryover, ensuring high-quality results in protein purification.

✓The optimal density of the butyl groups in the stationary phase offers high-resolution separation, making these columns ideal for complex biologics.

✓Distinct from other hydrophobic interaction chromatography (HIC) columns, they provide unique selectivity and superior performance in hydrophobic sample analysis.

✓Adhering to stringent product standards, these columns enhance the reliability and consistency of your chromatography workflows.

Supported by comprehensive services, MAbPac HIC-30 columns ensure optimal performance in protein interaction and purification applications.

Request a Quote

Guide for selecting the appropriate HPLC column for your analysis

Here are three ways to search for your ideal HPLC column

Find an equivalent column to the one you’re currently using

Identify the best column available for your current USP method

Locate the most suitable column for your newly developed method

Find your HPLC column with ease ›

Resources

Watch on-demand Analytical and life science Webinars

Detailed workflows for achieving robust and sensitive separation of monoclonal antibodies (mAbs), intact proteins, and their subunits and peptides using capillary chromatography coupled with liquid chromatography-mass spectrometry (LC-MS)

Discover the techniques to excel in comprehensive characterization of therapeutic antibodies at the protein, subunit, and peptide levels. Utilize the capillary Thermo Scientific MabPac RP column in either stand-alone or integrated EASY-Spray format, coupled with liquid chromatography-mass spectrometry (LC-MS), to achieve detailed and accurate analysis.