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Molecular Spectroscopy
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Table of contents
Featured NanoDrop instruments resources
Simple, easy, and fast nucleic acid and protein quantification
Accurate nucleic acid and protein quantification via UV absorbance
View more sample data in the Applications section
Download the NanoDrop One nucleic acid tech note
To learn more about interpreting purity ratios, see our technical note on nucleic acid purity
Documents for the NanoDrop Ultra/Ultraᶜ/Ultra FL/Ultraᶜ FL
Application notes and datasheets
NanoDrop Ultra Acclaro Pro performance data
NanoDrop Ultra fluorescence performance data
Nanodrop Ultra qPCR Recipe Calculator
NanoDrop Ultra nucleic acid performance data
NanoDrop Ultra protein A280 performance data
Protocols
Picogreen protocol for the Nanodrop Ultra
NanoDrop Ultra spectrophotometers and fluorometers BCA protein assay
NanoDrop Ultra spectrophotometers and fluorometers cuvette check
NanoDrop Ultra spectrophotometers and fluorometers pedestal image check
NanoDrop Ultra spectrophotometers and fluorometers Lowry protein assay
NanoDrop Ultra spectrophotometers and fluorometers pedestal absorbance measurements
NanoDrop Ultra spectrophotometers and fluorometers pierce 660 protein assay
NanoDrop Ultra spectrophotometers and fluorometers intensity check
User documents
NanoDrop Ultra user guide
NanoDrop Ultra user guide - Korean
NanoDrop Ultra user guide - Spanish
NanoDrop Ultra user guide - Polish
NanoDrop Ultra user guide - Japanese
NanoDrop Ultra user guide - Chinese
NanoDrop Ultra user guide - French
NanoDrop Ultra user guide - German
NanoDrop Ultra user guide - Italian
NanoDrop Ultra & SciVault 2 21 CFR part 11 compliance
NanoDrop Ultra API reference manual
SciVault 2 user guide
NanoDrop Ultra safety information
Cloud connectivity with the NanoDrop Ultra UV-Vis spectrophotometers and fluorometers
NanoDrop Ultra spectrophotometers and fluorometers instrument return instructions
NanoDrop Ultra unpack guide
NDU_Help_output_Italian
NDU_Help_output_German
NDU_Help_output_French
NDU_Help_output_Chinese
Brochures
NanoDrop Ultra microvolume UV-Vis spectrophotometers and fluorometers
Documents for the NanoDrop Eight Spectrophotometer
User guides
NanoDrop Eight User Guide - English
NanoDrop Eight User Guide - Chinese
NanoDrop Eight User Guide - Italian
Brochures & handbooks
NanoDrop Eight
Thermo Scientific NanoDrop products
Thermo Scientific NanoDrop Products (Korean)
NanoDrop Nucleic Acid Handbook
NanoDrop Protein A280 Handbook
NanoDrop Colorimetric Protein Assays Handbook
Application notes
Examining the efficiency of drug encapsulation within silk fibroin biomaterials
The MIQE Guidelines and Assessment of Nucleic Acids Prior to qPCR and RT-qPCR
Quality Control of Small Molecule Pharmaceuticals Using Spectroscopy
Purity Comparison of Automated Extraction Kits for HLA Typing
mRNA vaccine production: ensure quality control with the NanoDrop Spectrophotometers
Quantifying soy DNA extracts for downstream GMO detection
Measuring modified mRNA concentration for therapeutics
Technical notes
Sample Loading Tips for NanoDrop Eight Spectrophotometer
Custom Method for colorimetric quantification of cellular cytotoxicity
NanoDrop Eight Nucleic Acid Performance Data
The NanoDrop Eight Spectrophotometer detects contaminating nucleic acids in mammalian DNA and RNA preparations
NanoDrop Eight Protein Performance Data
NanoDrop Eight System Identifies Protein and Phenol in DNA Samples
Frequently asked questions (FAQs)
NanoDrop One Frequently Asked Questions (FAQs)
NanoDrop Instruments Service FAQ
Documents for the NanoDrop Lite Plus Spectrophotometer
User guides
NanoDrop Lite Plus User Guide - English
NanoDrop Lite Plus User Guide - Chinese
NanoDrop Lite Plus User Guide - Italian
NanoDrop Lite Plus Quick Start Guide - English
Brochures & handbooks
NanoDrop Lite Plus UV Microvolume Spectrophotometer
Thermo Scientific NanoDrop products
Thermo Scientific NanoDrop Products (Korean)
Technical notes
NanoDrop Lite Plus maintenance instructions
Frequently asked questions (FAQs)
NanoDrop Instruments Service FAQ
Application notes
Application note: The MIQE Guidelines and Assessment of Nucleic Acids Prior to qPCR and RT-qPCR
Application note: Evaluating DNA Purity for Molecular Cloning QC
Application note: NanoDrop Lite Plus Performance Data: Nucleic Acid and Protein A280
Lesson plan: Quantifying strawberry DNA with spectrophotometry
Publications
Teaching tomorrow’s scientists today: Lesson plans and resources for UV-Visible spectroscopic techniques
NanoDrop spectrophotometer and fluorometer videos
NanoDrop spectrophotometer and fluorometer webinars
Support and educational resources for NanoDrop spectrophotometers and fluorometers
Resources for discontinued NanoDrop instruments
User guides
NanoDrop One User Guide - English
NanoDrop One User Guide - Chinese
NanoDrop One User Guide - French
NanoDrop One User Guide - German
NanoDrop One User Guide - Italian
NanoDrop One User Guide - Japanese
NanoDrop One User Guide - Korean
NanoDrop One User Guide - Polish
NanoDrop One User Guide - Spanish
Manuals & guides
NanoDrop One-1000-2000 Comparison Guide
NanoDrop Products Selection Guide
NanoDrop One Standard Warranty
Brochures & handbooks
NanoDrop One Microvolume UV-Vis Spectrophotometer Brochure
NanoDrop One Specifications
Thermo Scientific NanoDrop products
Thermo Scientific NanoDrop Products (Korean)
NanoDrop Nucleic Acid Handbook
NanoDrop Protein A280 Handbook
NanoDrop Colorimetric Protein Assays Handbook
Application notes
The MIQE Guidelines and Assessment of Nucleic Acids Prior to qPCR and RT-qPCR
Analyze supercoiled plasmids with the NanoDrop One/Oneᶜ Spectrophotometer
Macroalgae DNA quantification and contaminant characterization with the NanoDrop One Spectrophotometer
Next generation sequencing sample preparation with the NanoDrop One Spectrophotometer
Monitoring enzyme kinetics with the NanoDrop Oneᶜ Spectrophotometer
Measurement of poly(lactic-co-glycolic acid) nanoparticle concentration with the NanoDrop One Microvolume UV-Vis Spectrophotometer
Observation of gold nanoshell plasmon resonance shifts after bioconjugation
Quantification of gold nanoparticles using the NanoDrop One Microvolume UV-Vis Spectrophotometer
Detection of RNA Contamination in Mammalian DNA Preparations
Quantification of gold nanoshells using the NanoDrop One Microvolume UV-Vis Spectrophotometer
Using NanoDrop One/Oneᶜ to determine phenol and protein contaminants in nucleic acids for RT-qPCR quality control
Using the NanoDrop One/Oneᶜ Spectrophotometer to determine DNA/RNA contamination in nucleic acids for RT-qPCR/qPCR quality control
Using the NanoDrop One to Quantify Protein and Peptide Preparations at 205 nm
Quantify Chlorophyll a and Chlorophyll b With a Custom Method
Quantifying Glucose Concentration Using a Hexokinase Assay
A Custom Method for Hemoglobin Measurements
mRNA vaccine production: ensure quality control with the NanoDrop Spectrophotometers
Structural Biology Workflows (NMR, XRC, cryo-EM) with NanoDrop Spectrophotometers
Measuring modified mRNA concentration for therapeutics
Technical notes
Comparing Microvolume and Cuvette Based Measurements of Microbial Cell Cultures
Custom Method for colorimetric quantification of cellular cytotoxicity
A custom method for endotoxin quantification
Blanking with high absorbing buffers such as RIPA negatively affects Protein A280 measurements
NanoDrop One Performance Data - Nucleic Acid Measurement at 260 nm
Acclaro Protein Contaminant ID: Detection of Protein in Nucleic Acid Samples Using the NanoDrop One Spectrophotometer
Sample Reproducibility for NanoDrop Spectrophotometers
Nucleic Acid Controls vs. Standards
Assessment of Nucleic Acid Purity Ratios
Influence of Buffer on Choice of Protein Quantification Method
Best Practices: Proteins
Bradford Assay Tips
Solvent Compatibility for NanoDrop products pedestal surfaces
Acclaro phenol contaminant ID - NanoDrop One Tech Note
Performance verification check for NanoDrop One/OneC
Performing an intensity check for NanoDrop One/OneC
Performing an pedestal check for NanoDrop One/OneC
Protocols
Pierce 660 nm Protein Assay
Protein Lowry Assay
Posters & publications
Teaching tomorrow’s scientists today: Lesson plans and resources for UV-Visible spectroscopic techniques
Gold Nanoparticle and Gold Nanoshell Analysis with a NanoDrop One Microvolume UV-Vis Spectrophotometer
Qualification of nucleic acid samples for infectious disease research workflows
Frequently asked questions (FAQs)
Security Suite Software for Evolution series and NanoDrop One/OneC Spectrophotometers Frequently Asked Questions (FAQs)
NanoDrop One Frequently Asked Questions (FAQs)
NanoDrop Instruments Service FAQ
Acclaro Frequently Asked Questions - NanoDrop One Sample Contaminant Identification
Lesson plans
Activity of acid phosphatase extracted from wheat germ
Infographics
The journey to 100,000 NanoDrops starts with a single microliter.
Application notes
HLA Typing: Template Quality Control for SSP and SSO
Microgenomic Expression Profiling
Nucleic Acid and Fluorescent Dye Concentration Measurement
Quantitative Real Time RT PCR
Small Molecule Crystallography
Brochures & handbooks
NanoDrop Colorimetric Protein Assays Handbook
NanoDrop Nucleic Acid Handbook
NanoDrop Protein A280 Handbook
Frequently asked questions (FAQs)
NanoDrop 1000 Frequently Asked Questions
NanoDrop Instruments Service FAQ
NanoDrop One Frequently Asked Questions (FAQs)
Manuals & guides
NanoDrop 1000 User Manual
NanoDrop One-1000-2000 Comparison Guide
NanoDrop Products Selection Guide
Technical notes
Archived Data for NanoDrop 1000
Assessment of Nucleic Acid Purity Ratios
Best Practices: Proteins
Blank Measurements
Bradford Assay Tips
Homogeneity Study– Effect on Reproducibility - NanoDrop 1000
Influence of Buffer on Choice of Protein Quantification Method
Liquid Column Breakage Warning
NanoDrop 1000 Reproducibility Studies
Nucleic Acid Controls vs. Standards
Sample Reproducibility for NanoDrop Spectrophotometers
Solenoid cleaning procedure for the NanoDrop 1000
Solvent Compatibility for NanoDrop products pedestal surfaces
USB Connectivity Issues with NanoDrop 1000
Application notes
Detection and Avoidance of Polysaccharides in Plant Nucleic Acid Extractions
Measuring Viability Decline in Animal Cell Suspension Cultures Using NanoDrop 2000
MIQE Guidelines and Assessment of Nucleic Acids Prior to qPCR and RT-qPCR
Pierce 660 nm Micro-Volume Assay Advantages
Quality Control of Industrial Dye Products Using Absorbance Spectroscopy
Quality Control during Library Construction for de novo Next-Gen Sequencing Platforms
Quality Control of Small Molecule Pharmaceuticals Using Spectrophotometry
Use of Custom Methods Capability of NanoDrop Spectrophotometers for Toxicology Studies
Brochures & handbooks
NanoDrop Colorimetric Protein Assays Handbook
NanoDrop Nucleic Acid Handbook
NanoDrop Protein A280 Handbook
Thermo Scientific NanoDrop products
Frequently asked questions (FAQs)
NanoDrop Instruments Service FAQ
NanoDrop One Frequently Asked Questions (FAQs)
Protein Absorbance Measurements Frequently Asked Questions - NanoDrop 2000/2000c
Manuals & guides
NanoDrop 2000 / 2000c User Manual
NanoDrop One-1000-2000 Comparison Guide
NanoDrop Products Selection Guide
Posters & publications
Analyzing Differences in Bacterial Density Measurements between Spectrophotometers
Microvolume Quantification of Nucleic Acids in Molecular Diagnostics (Poster)
Protocols
Bradford Protein Assay for NanoDrop 2000/2000C
Pierce 660 nm Protein Assay - NanoDrop 2000/2000c
Technical notes
A205 Custom Method for Protein and Peptide Quantification
Accuracy vs. Reference Spectrophotometer - NanoDrop 2000/2000c
Activity Measurements of Pentose Phosphate Pathway Enzymes Using the NanoDrop 2000c Kinetics Application
Assessment of Nucleic Acid Purity
Assessment of Nucleic Acid Purity Ratios
Baseline Corrections for Nucleic Acid and Protein A280 Measurements
Best Practices: Proteins
Blank Measurements
Bradford Assay Tips
Calibration Check Procedure for NanoDrop 2000, NanoDrop 2000c and NanoDrop 1000
Cleaning and Reconditioning for the NanoDrop 2000 / 2000c
Clearing Error 1010 for Windows 7 for NanoDrop 2000/2000c
Colorimetric Protein Assays - Quick Start Protocol
Comparing Microvolume and Cuvette Based Measurements of Microbial Cell Cultures
Influence of Buffer on Choice of Protein Quantification Method
Liquid Column Breakage Warning
Lowry Assay: Generation of a Standard Curve Based Custom Method Using NanoDrop 2000/2000C
Microvolume Quantification of Proteins by UV-Vis Absorbance or Fluorescence
NanoDrop 2000/2000c Protein Assay Guide
NanoDrop 2000 Reproducibility Studies for dsDNA
Nucleic Acid Controls vs. Standards
Protein Measurement Accuracy and Reproducibility - NanoDrop 2000
Protocol for BCA Protein Assay Using a NanoDrop 2000/2000c Spectrophotometer
Sample Reproducibility for NanoDrop Spectrophotometers
Specific NanoDrop Instrument Recommendations to Achieve Accurate Oligonucleotide Quantification Results
Solvent Compatibility for NanoDrop products pedestal surfaces
Application notes
Measuring Enzyme Activity using Thermo Scientific NanoDrop 3300 Fluorospectrometer
Molecular Beacon Probes: Micro-Volume Fluorescence Measurement of HPLC Isolated Probes
Using the NanoDrop 3300 Fluorospectrometer for Cancer Research Applications
Using the NanoDrop 3300 Fluorospectrometer For Micro-Volume Fluorescence/FRET Applications
Using the NanoDrop 3300 Fluorospectrometer For Nanoparticle/Quantum Dot Applications
Brochures & handbooks
Thermo Scientific NanoDrop products
Thermo Scientific NanoDrop Products (Korean)
Frequently asked questions (FAQs)
NanoDrop 3300 FAQs for Protein Measurements
NanoDrop Instruments Service FAQ
Manuals & guides
User Manual for the NanoDrop 3300 fluorospectrometer
NanoDrop 3300 Fluorospectrometer specifications
NanoDrop Products Selection Guide
Protocols
AccuBlue Broad Range dsDNA Assay for NanoDrop 3300 Fluorospectrometer Assay
AccuBlue High Sensitivity dsDNA Assay for NanoDrop 3300 Fluorospectrometer
Fluoraldyhyde OPA Protein Assay for NanoDrop 3300 Fluorospectrometer
Fluorescamine Protein Assay for NanoDrop 3300 Fluorospectrometer
FluoroProfile Protein Assay for NanoDrop 3300 Fluorospectrometer
Hoechst dsDNA 33258 Assay for NanoDrop 3300 Fluorospectrometer
PicoGreen dsDNA Assay for NanoDrop 3300 Fluorospectrometer
Quant-iT dsDNA Broad Range Assay for NanoDrop 3300 Fluorospectrometer
Quant-iT dsDNA High Sensitivity Assay for NanoDrop 3300 Fluorospectrometer
Ribogreen RNA Assay for NanoDrop 3300 Fluorospectrometer
Sybr Green I dsDNA Assay for NanoDrop 3300 Fluorospectrometer
Sybr Gold ssDNA Assay for NanoDrop 3300 Fluorospectrometer
Technical notes
4– Methyl Umbelliferone Assay Linearity, Reproducibility and Sensitivity
Alexa 555 Assay Linearity, Reproducibility and Sensitivity
Alexa 647 Assay Linearity, Reproducibility and Sensitivity
Archived Data NanoDrop 3300 Fluorospectrometer
Best Practices: Proteins
Error Code 8 for NanoDrop 3300
FITC Linearity, Reproducibility and Sensitivity
Fluorescence-based Nucleic Acid Assays
Fluorescence-based Protein Assays
Gain Settings for the NanoDrop 3300
Hoechst Assay Linearity, Reproducibility and Sensitivity
Measuring Mouse GFR by FITC-Inulin using the Thermo Scientific NanoDrop 3300 Fluorospectrometer
NanoDrop 3300 Virtual Filtering
NanoDrop Instruments calibration guidance
PicoGreen® Assay Linearity, Reproducibility and Sensitivity
Quantum Dots Assay Linearity, Reproducibility and Sensitivity
Quinine Sulfate Assay Linearity, Reproducibility and Sensitivity
Reproducibility and Linearity Assessment Using the Blue LED
RiboGreen® Assay Linearity, Reproducibility and Sensitivity
Sample Reproducibility for NanoDrop Spectrophotometers
Solvent Compatibility for NanoDrop products pedestal surfaces
Videos
A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
Application notes
MIQE Guidelines and Assessment of Nucleic Acids Prior to qPCR and RT-qPCR
Pierce 660 nm Micro-Volume Assay Advantages
Brochures & handbooks
NanoDrop Nucleic Acid Handbook
NanoDrop Protein A280 Handbook
NanoDrop Colorimetric Protein Assays Handbook
Frequently asked questions (FAQs)
NanoDrop Instruments Service FAQ
NanoDrop One Frequently Asked Questions (FAQs)
Manuals & guides
NanoDrop 8000 User Manual
NanoDrop Products Selection Guide
Technical notes
Archived Data for NanoDrop 8000
Assessment of Nucleic Acid Purity Ratios
Baseline Corrections for Nucleic Acid and Protein A280 Measurements
Best Practices: Proteins
Blank Measurements
Bradford Assay Tips
Bradford Protein Assay Protocol for the NanoDrop 8000 Spectrophotometer
Calibration Check Procedure for NanoDrop 2000, NanoDrop 2000c and NanoDrop 1000
Colorimetric Protein Assays - Quick Start Protocol
Comparing Microvolume and Cuvette Based Measurements of Microbial Cell Cultures
Influence of Buffer on Choice of Protein Quantification Method
Liquid Column Breakage Warning
Microvolume Quantification of Proteins by UV-Vis Absorbance or Fluorescence
NanoDrop 8000 Linearity Studies
NanoDrop 8000 Sample Carryover
Nucleic Acid Controls vs. Standards
Pierce 660 nm Protein Assay Protocol for the NanoDrop 8000 Spectrophotometer
Protocol for BCA Protein Assay Using a NanoDrop 8000 Spectrophotometer
Protein Measurement Accuracy and Reproducibility - NanoDrop 2000
Sample ID and Plate Set-Up Guide - NanoDrop 8000
Sample Reproducibility for NanoDrop Spectrophotometers
Solvent Compatibility for NanoDrop products pedestal surfaces
Videos
NanoDrop 8000 Calibration Check Best Practices Video
Brochures & handbooks
NanoDrop Protein A280 Handbook
NanoDrop Nucleic Acid Handbook
Frequently asked questions (FAQs)
NanoDrop Instruments Service FAQ
NanoDrop Lite Tech Support FAQs
NanoDrop One Frequently Asked Questions (FAQs)
Manuals & guides
NanoDrop Lite Printer Quick Reference
NanoDrop Lite Quick Reference
NanoDrop Lite User Manual
NanoDrop Products Selection Guide
Technical notes
Assessment of Nucleic Acid Purity Ratios
Best Practices: Proteins
Blank Measurements
Calibration Check Outliers - NanoDrop Lite
Calibration Check Procedure for NanoDrop Lite spectrophotometer
Cleaning and Reconditioning for the NanoDrop Lite spectrophotometer
Influence of Buffer on Choice of Protein Quantification Method
Influence of Buffer on Protein Quantification ay A280 - NanoDrop Lite
Interpretation of Nucleic Acid Purity Ratios for NanoDrop Lite
Liquid Column Breakage Warning
NanoDrop Lite Data Management and Transfer
Nucleic Acid Controls vs. Standards
Sample Reproducibility for NanoDrop Spectrophotometers
Solvent Compatibility for NanoDrop products pedestal surfaces