Expand your violet laser capabilities

The 2018 Invitrogen Attune NxT Flow Cytometer with acoustic focusing technology is now available with 6 fluorescence detectors off the violet laser, allowing measurement of up to 11 fluorescence parameters with a 2-laser instrument, 12 fluorescence parameters with a 3-laser instrument, or 14 fluorescence parameters with a 4-laser instrument (Table 1).

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Table 1. Attune NxT Flow Cytometer configuration using six fluorescence detectors for the violet laser.

Laser Fluorescence detectors
2-laser 3-laser 4-laser
Violet, 405 nm 6 6 6
Blue, 488 nm 3 3 2
Yellow, 561 nm NA NA 3
Red, 637 nm NA 3 3
Total fluorescence detectors available 9 12 14
Total parameters per configuration* 11 14 16

*Includes forward scatter (FSC) and side scatter (SSC). NA = not applicable.

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Table 2. Attune NxT Flow Cytometer configuration options.

Lasers Laser configuration
(Cat. No.)
Lasers included, No. of detection channels Total detection channels*
Violet, 405 nm Blue, 488 nm Yellow, 561 nm Green,
532 nm
Red, 637 nm
1 Blue
(A24864)
Available as upgrade†
4
Available as upgrade† Available as upgrade† Available as upgrade† 6
2 Blue/green
(A28995)
Available as upgrade†
3
 
4
Available as upgrade† 9
Blue/yellow
(A24861)
Available as upgrade†
3
4
  Available as upgrade† 9
Blue/red
(A24863)
Available as upgrade†
4
Available as upgrade† Available as upgrade†
3
9
Blue/violet
(A24862)
4
4
Available as upgrade† Available as upgrade† Available as upgrade† 10
Blue/violet 6
(A29002)
6
3
Available as upgrade†   Available as upgrade† 11
3 Blue/green/red
(A28997)
Available as upgrade†
3
 
4
3
12
Blue/red/yellow (A28993) Available as upgrade† 3 4   3 12
Blue/green/violet
(A28999)
4
3
 
4
Available as upgrade† 13
Blue/violet/yellow (A24859)
4
3
4
 
Available as upgrade† 13
Blue/red/violet
(A24860)
4
4
Available as upgrade† Available as upgrade†
3
13
Blue/red/violet 6
(A29003)
6
3
Available as upgrade†  
3
14
4 Blue/red/violet/green
(A29001)
4
3
 
4
3
16
Blue/red/yellow/violet
(A24858)
4
3
4
 
3
16
Blue/red/yellow/violet 6
(A29004)
6
2
3
 
3
16
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel.
† Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser UpgradeRed Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options.

The 2018 Attune NxT Flow Cytometer 6-channel violet laser configuration accommodates the Invitrogen eBioscience Super Bright antibody conjugates along with other commercially available violet-excitable fluorophores.

Detector Bandpass (nm) Fluorophores*
VL1 450/40 Super Bright 436, Brilliant Violet 421, eFluor 450, Pacific Blue, BD Horizon V450, VioBlue
VL2 525/50 eFluor 506, Brilliant Violet 510, Pacific Green, BD Horizon V500, VioGreen
VL3 610/20 Super Bright 600, Brilliant Violet 605, Pacific Orange
VL4 660/20 Super Bright 645, Brilliant Violet 650
VL5 710/50 Super Bright 702, Brilliant Violet 711
VL6 780/60 Brilliant Violet 786

* List is not inclusive of all available fluorophores.

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Table 2. Attune NxT Flow Cytometer configuration options.

Lasers Laser configuration
(Cat. No.)
Lasers included, No. of detection channels Total detection channels*
Violet, 405 nm Blue, 488 nm Yellow, 561 nm Green,
532 nm
Red, 637 nm
1 Blue
(A24864)
Available as upgrade†
4
Available as upgrade† Available as upgrade† Available as upgrade† 6
2 Blue/green
(A28995)
Available as upgrade†
3
 
4
Available as upgrade† 9
Blue/yellow
(A24861)
Available as upgrade†
3
4
  Available as upgrade† 9
Blue/red
(A24863)
Available as upgrade†
4
Available as upgrade† Available as upgrade†
3
9
Blue/violet
(A24862)
4
4
Available as upgrade† Available as upgrade† Available as upgrade† 10
Blue/violet 6
(A29002)
6
3
Available as upgrade†   Available as upgrade† 11
3 Blue/green/red
(A28997)
Available as upgrade†
3
 
4
3
12
Blue/red/yellow (A28993) Available as upgrade† 3 4   3 12
Blue/green/violet
(A28999)
4
3
 
4
Available as upgrade† 13
Blue/violet/yellow (A24859)
4
3
4
 
Available as upgrade† 13
Blue/red/violet
(A24860)
4
4
Available as upgrade† Available as upgrade†
3
13
Blue/red/violet 6
(A29003)
6
3
Available as upgrade†  
3
14
4 Blue/red/violet/green
(A29001)
4
3
 
4
3
16
Blue/red/yellow/violet
(A24858)
4
3
4
 
3
16
Blue/red/yellow/violet 6
(A29004)
6
2
3
 
3
16
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel.
† Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser UpgradeRed Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options.

The 2018 Attune NxT Flow Cytometer 6-channel violet laser configuration accommodates the Invitrogen eBioscience Super Bright antibody conjugates along with other commercially available violet-excitable fluorophores.

Detector Bandpass (nm) Fluorophores*
VL1 450/40 Super Bright 436, Brilliant Violet 421, eFluor 450, Pacific Blue, BD Horizon V450, VioBlue
VL2 525/50 eFluor 506, Brilliant Violet 510, Pacific Green, BD Horizon V500, VioGreen
VL3 610/20 Super Bright 600, Brilliant Violet 605, Pacific Orange
VL4 660/20 Super Bright 645, Brilliant Violet 650
VL5 710/50 Super Bright 702, Brilliant Violet 711
VL6 780/60 Brilliant Violet 786

* List is not inclusive of all available fluorophores.

ReagentsAntibodiesPanel Builder

Flow Panel Design Tool
 

Choose antibody conjugates for your panel in 3 steps: 1) pick the antibody species reactivity, 2) select up to 14 targets, and 3) choose the lasers or fluorophores you want to view.

Fluorescence SpectraViewer

This online tool allows researchers to assess the spectral compatibility of dyes and probes in the course of designing experiments that utilize fluorescence detection techniques.

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