Transformative 14-color cell analyzers with high-speed camera option

Both Attune models are efficient, flexible, transformative cell analyzers with acoustic focusing and up to 14 colors. Choose between the Attune CytPix Flow Cytometer with a high-speed camera and the Attune NxT Flow Cytometer without it.

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Which Attune flow cytometer is best for you?

Both Attune models have the same technology and share common features:

Acoustic focusing for sensitivity

Rare event detection

Novel optical design

Powerful, intuitive software

Fast, accurate acquisition

Designed for flexibility

Clog-resistant technology

Biosafety hood compatible

The Attune CytPix flow cytometer incorporates a high-speed, high-resolution, brightfield camera to capture images of cell populations as they pass through the flow cell. Combined with image processing on the Attune Cytometric Software, researchers can measure over 25 cell morphology parameters to gain novel insight into their sample. Combining morphology analysis with flow cytometric data improves data accuracy and precision.

Selection guide

Attune CytPix Flow Cytometer


Attune NxT Flow Cytometer

Product summary
Advanced, high throughput, acoustic focusing, benchtop flow cytometer with up to 14 colors and brightfield imaging capability Advanced, high throughput, acoustic focusing, benchtop flow cytometer with up to 14 colors
Example usage
All flow cytometric cell analysis applications, including those that need visual confirmation of cell identity and morphology; researchers can confirm gate accuracy, observe cell-to-cell interactions, and visualize structural features in cells of interest All flow cytometric cell analysis applications 

Comparative highlights

For complete specifications and further information, click the model name in the table below.



Attune CytPix Flow Cytometer

Attune NxT Flow Cytometer


Number of lasers



Number of detection channels

2 scatter channels, up to 14 fluorescence channels

Imaging illumination

405 nm laser with <50 nanosecond pulse width


Optical alignment

Fixed alignment with pre-aligned welded fiber; no user maintenance required


Acoustic focusing

Custom sample flow rates

Performance (fluorescence detection)

Fluorescence sensitivity

≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC

Fluorescence resolution

CV <3% for the singlet peak of propidium iodide-stained chicken erythrocyte nuclei (CEN)

Maximum electronic speed

Up to 35,000 events/sec with all parameters

Performance (imaging)

Image capture rate

Up to 6,000 images/sec depending on image size and event rate



Magnification 20x,
Numerical aperture 0.45


Pixel resolution

0.3 microns/pixel


Detection limit

Visually detect 800 nm particles


Image analysis Models leukocytes, beads N/A
Parameters/Features More than 25 measured image parameters in 5 categories: shape features, intensity features, object features, pixel features, and system features N/A
Sample size range of models 5–20 µm N/A
Image processing speed Fast: 1,000 images/second
Standard: (varies by image size and complexity)

Quality and regulatory

Instrument tracking

Automated daily baseline and performance test with Levey-Jennings plots

Regulatory status

For Research Use Only


Dimensions (H x W x D) including fluid bottles

~49 x 58 x 43 cm;
~19 x 23 x 17 in

~40 x 58 x 43 cm;
~16 x 23 x 17 in

Biosafety hood compatibility



64 GB (4 x 16 GB) DDR4 2666 MHz UDIMM Non-ECC

32 GB

Hard drives

2 x 8TB SSD, 2.5-inch Samsung 870 QVO, 560 MB/s

2 x 2TB SATA 3.0 GB/s, 8MB data burst cache; Controller RAID1, integrated

Graphics processor

Nvidia Quadro P2200


Laser and detector configurations

Lasers Laser configuration Number of detection channels for included lasers Total detection channels* Attune CytPix Attune NxT
Violet, 405nm Blue, 488nm Yellow, 561nm Green, 532nm Red, 637nm
1 Blue
4 6 N/A A24864
2 Blue/green
3 4 9 N/A A28995
3 4 9 A51842 A24861
4 3 9 A51840 A24863
4 4 10 A51841 A24862
Blue/violet 6
6 3 11 A51843 A29002
3 Blue/green/red
3 4 3 12 N/A A28997
3 4 3 12 A51845 A28993
4 3 4 13 N/A A28999
4 3 4 13 A51846 A24859
4 4 3 13 A51844 A24860
Blue/red/violet 6
6 3 3 14 A51847 A29003
4 Blue/red/violet/green
4 3 4 3 16 N/A A29001
4 3 4 3 16 A51848 A24858
Blue/red/yellow/violet 6
6 2 3 3 16 A51849 A29004

For Research Use Only, not intended for use in diagnostic procedures.