Both Attune models are efficient, flexible, transformative cell analyzers with acoustic focusing and up to 14 colors. Choose between the Attune CytPix Flow Cytometer with a high-speed camera and the Attune NxT Flow Cytometer without it.
Both Attune models have the same technology and share common features:
Acoustic focusing for sensitivity
Rare event detection
Novel optical design
Powerful, intuitive software
Fast, accurate acquisition
Designed for flexibility
Clog-resistant technology
Biosafety hood compatible
The Attune CytPix flow cytometer incorporates a high-speed, high-resolution, brightfield camera to capture images of cell populations as they pass through the flow cell. Combined with image processing on the Attune Cytometric Software, researchers can measure over 25 cell morphology parameters to gain novel insight into their sample. Combining morphology analysis with flow cytometric data improves data accuracy and precision.
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| Product summary | |
| Advanced, high throughput, acoustic focusing, benchtop flow cytometer with up to 14 colors and brightfield imaging capability | Advanced, high throughput, acoustic focusing, benchtop flow cytometer with up to 14 colors |
| Example usage | |
| All flow cytometric cell analysis applications, including those that need visual confirmation of cell identity and morphology; researchers can confirm gate accuracy, observe cell-to-cell interactions, and visualize structural features in cells of interest | All flow cytometric cell analysis applications |
For complete specifications and further information, click the model name in the table below.
Category |
Feature |
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|---|---|---|---|
Optics |
Number of lasers |
2–4 |
1–4 |
Number of detection channels |
2 scatter channels, up to 14 fluorescence channels |
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Imaging illumination |
405 nm laser with <50 nanosecond pulse width |
N/A |
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Optical alignment |
Fixed alignment with pre-aligned welded fiber; no user maintenance required |
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Fluidics |
Acoustic focusing |
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Custom sample flow rates |
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Performance (fluorescence detection) |
Fluorescence sensitivity |
≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC |
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Fluorescence resolution |
CV <3% for the singlet peak of propidium iodide-stained chicken erythrocyte nuclei (CEN) |
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Maximum electronic speed |
Up to 35,000 events/sec with all parameters |
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Performance (imaging) |
Image capture rate |
Up to 6,000 images/sec depending on image size and event rate |
N/A |
Objective |
Magnification 20x, |
N/A |
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Pixel resolution |
0.3 microns/pixel |
N/A |
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Detection limit |
Visually detect 800 nm particles |
N/A |
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| Image analysis | Models | leukocytes, beads | N/A |
| Parameters/Features | More than 25 measured image parameters in 5 categories: shape features, intensity features, object features, pixel features, and system features | N/A | |
| Sample size range of models | 5–20 µm | N/A | |
| Image processing speed | Fast: 1,000 images/second Standard: (varies by image size and complexity) |
N/A | |
Quality and regulatory |
Instrument tracking |
Automated daily baseline and performance test with Levey-Jennings plots |
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Regulatory status |
For Research Use Only |
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Physical |
Dimensions (H x W x D) including fluid bottles |
~49 x 58 x 43 cm; |
~40 x 58 x 43 cm; |
Biosafety hood compatibility |
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Computer |
Memory |
64 GB (4 x 16 GB) DDR4 2666 MHz UDIMM Non-ECC |
32 GB |
Hard drives |
2 x 8TB SSD, 2.5-inch Samsung 870 QVO, 560 MB/s |
2 x 2TB SATA 3.0 GB/s, 8MB data burst cache; Controller RAID1, integrated |
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Graphics processor |
Nvidia Quadro P2200 |
N/A |
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| Lasers | Laser configuration | Number of detection channels for included lasers | Total detection channels* | Attune CytPix | Attune NxT | ||||
|---|---|---|---|---|---|---|---|---|---|
| Violet, 405nm | Blue, 488nm | Yellow, 561nm | Green, 532nm | Red, 637nm | |||||
| 1 | Blue
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✝ | 4 | ✝ | ✝ | ✝ | 6 | N/A | A24864 |
| 2 | Blue/green
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✝ | 3 | 4 | ✝ | 9 | N/A | A28995 | |
| Blue/yellow
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✝ | 3 | 4 | ✝ | 9 | A51842 | A24861 | ||
| Blue/red
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✝ | 4 | ✝ | ✝ | 3 | 9 | A51840 | A24863 | |
| Blue/violet
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4 | 4 | ✝ | ✝ | ✝ | 10 | A51841 | A24862 | |
| Blue/violet 6
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6 | 3 | ✝ | ✝ | 11 | A51843 | A29002 | ||
| 3 | Blue/green/red
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✝ | 3 | 4 | 3 | 12 | N/A | A28997 | |
| Blue/red/yellow
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✝ | 3 | 4 | 3 | 12 | A51845 | A28993 | ||
| Blue/green/violet
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4 | 3 | 4 | ✝ | 13 | N/A | A28999 | ||
| Blue/violet/yellow
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4 | 3 | 4 | ✝ | 13 | A51846 | A24859 | ||
| Blue/red/violet
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4 | 4 | ✝ | ✝ | 3 | 13 | A51844 | A24860 | |
| Blue/red/violet 6
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6 | 3 | ✝ | 3 | 14 | A51847 | A29003 | ||
| 4 | Blue/red/violet/green
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4 | 3 | 4 | 3 | 16 | N/A | A29001 | |
| Blue/red/yellow/violet
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4 | 3 | 4 | 3 | 16 | A51848 | A24858 | ||
| Blue/red/yellow/violet 6
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6 | 2 | 3 | 3 | 16 | A51849 | A29004 | ||
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*Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. ✝Laser upgrade available; green laser not available on Attune CytPix |
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For Research Use Only, not intended for use in diagnostic procedures.
