Attune Flow Cytometer Models

Feature

Attune CytPix Flow Cytometer

Attune NxT Flow Cytometer

Optics

Number of lasers

2–4

1–4

Number of detection channels

2 scatter channels, up to 14 fluorescence channels

Imaging illumination

405 nm laser with <50 nanosecond pulse width

N/A

Optical alignment

Fixed alignment with pre-aligned welded fiber; no user maintenance required

Fluidics

Acoustic focusing

Custom sample flow rates

Performance (fluorescence detection)

Fluorescence sensitivity

≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC

Fluorescence resolution

CV <3% for the singlet peak of propidium iodide-stained chicken erythrocyte nuclei (CEN)

Maximum electronic speed

Up to 35,000 events/sec with all parameters

Performance (imaging)

Image capture rate

Up to 6,000 images/sec depending on image size and event rate

N/A

Objective

Magnification 20x,
Numerical aperture 0.45

N/A

Pixel resolution

0.3 microns/pixel

N/A

Detection limit

Visually detect 800-nm particles

N/A

Quality and regulatory

Instrument tracking

Automated daily baseline and performance test with Levey-Jennings plots

Regulatory status

For Research Use Only

Physical

Dimensions (H x W x D) including fluid bottles

~49 x 58 x 43 cm;
~19 x 23 x 17 in

~40 x 58 x 43 cm;
~16 x 23 x 17 in

Biosafety hood compatibility

Computer

Memory

64 GB (4 x 16 GB) DDR4 2666 MHz UDIMM Non-ECC

32 GB

Hard drives

2 x 8TB SSD, 2.5-inch Samsung 870 QVO, 560 MB/s

2 x 2TB SATA 3.0 GB/s, 8MB data burst cache; Controller RAID1, integrated

Graphics processor

Nvidia Quadro P2200

N/A