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Restriction Enzymes Education |
Learn about the history of restriction enzymes, usage considerations, and troubleshooting. Discover their diverse applications beyond traditional cloning. These articles cover the essentials of restriction digestion, provide practical tips for set up and troubleshooting experiments, and offer expert advice on the applications of restriction enzymes.
Restriction enzyme basics
Take a trip down memory lane. Learn about the history, naming system, and classification of restriction enzymes.
Key considerations for reaction set-up
Practical advice and best practices for successful restriction enzyme reactions, including enzyme quality and behavior, star activity, methylation status, and reaction set-up.
Restriction enzyme troubleshooting guide
Troubleshooting guide offers solutions to common issues encountered when performing digestions with restriction endonucleases.
Scarless cloning
Learn about scarless cloning with Golden Gate assembly to construct large and complex DNA sequences.
Five clone screening methods
Read about common clone screening methods for successful verification of inserts.
Methylated DNA digestion
A complete guide to dam, dcm, and CpG methylation mechanisms, and cleavage effect of various enzymes on the methylated sites.
Genome mapping and analysis
Learn about restriction endonuclease applications in genome mapping and analysis such as RFLP, AFLP, COBRA, and qAMP.
Resources & support for restriction enzymes
Products related to restriction enzymes
Tools to help your restriction digest
For Research Use Only. Not for use in diagnostic procedures.