Invitrogen™

NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI)

Catalog number: R37606
Invitrogen™

NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI)

Catalog number: R37606
Catalog Number
R37606
Unit Size
1 kit
Price (USD)
Price: 148.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
R376061 kit
Price: 148.00
Your Price:
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Product Overview
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Additional Information
Recommendations
Recommendations
DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue™ Fixed Cell ReadyProbes™ Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells.

Also available: DAPI (4',6-diamidino-2-phenylindole, dihydrochloride) at 12.5X concentration (5 µM) in water.

• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area• DAPI is excited with UV light and detected through a blue/cyan filter

See other ReadyProbes™ reagents for cell staining
See other nuclear stains for imaging

Cell imaging applications
DAPI is a classic fluorescent dye used extensively for nuclear staining of fixed cells. In fluorescence microscopy, DAPI is excited with UV light and detected through a blue/cyan filter (Figure 1)..

Suggestions for use
In most cases 2 drops/ml of NucBlue™ Fixed Cell Stain and an incubation of 15 to 30 minutes will produce bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more, or fewer, drops until the optimal staining intensity is obtained.
• NucBlue™ Fixed Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter, blue GFP filters, or the Semrock BrightLine™ Alexa Fluor™ 350 Dye filter set.
• As a preferred blue nuclear stain in fixed cell imaging experiments, NucBlue™ Fixed Cell Stain is ideal for use with antibody-based applications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color
Blue
Excitation Wavelength Range
360⁄460
Dye Type
DAPI
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer
Quantity
6 vial(s)
Detection Method
Fluorescent
Emission
Visible
Form
Liquid
Product Line
Molecular Probes™
Shipping Condition
Room Temperature
Product Type
Dye
SubCellular Localization
Nucleus

Contents & Storage

6 × 2.5 mL dropper bottles
  • Store at ≤ 25°C
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