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Your journey to stem cell culture excellence begins with Gibco Education. Including both core concepts and laboratory techniques, these handbooks, virtual training modules, and how-to videos can help pave your way to success in PSC (pluripotent stem cell) research.
Each of these in-depth multimedia training modules covers principles and practices of PSC culture and differentiation. You can even test your learning at the end of each module.
Note: You need to be logged in to your Thermo Fisher Scientific on-line account to access the PSC training modules.
A detailed guide through our protocol for thawing induced pluripotent stem cells (iPSCs) using StemFlex Medium.
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A complete guide for gene editing in PSCs, including all the tools and protocols needed to design, deliver, and screen for preferred gene edits in PSCs.
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Preparation and maintenance of PSCs in suspension culture using the new Gibco StemScale PSC Suspension Medium.
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Learn how to develop neural organoids from PSCs following the steps of our application note.
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Get started with 3D cell culture models by exploring 3D cell culture background, model requirements, creation methods, and development considerations. Access course
A complete guide for reprogramming somatic cells into pluripotent stem cells (PSCs) using the CytoTune 2.0 Sendai Reprogramming Kit.
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An easy-to-use guide for the preparation and differentiation of human pluripotent stem cells into functional cardiomyocytes for basic and translational research.
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Expand your knowledge of PSC laboratory techniques with this collection of 3- to 5-minute videos demonstrating procedures such as:
This video demonstrates preparation and storage on complete Gibco StemFlex Medium for pluripotent stem cell maintenance.
This video shows how to correctly prepare Essential 8 Medium to support robust, feeder-free pluripotent stem cell culture. Includes important information about thawing and heating.
Watch and learn how to coat cell culture plates with Vitronectin XF, an extracellular matrix for transferring or expanding pluripotent stem cell colonies.
Perform routine passaging of pluripotent stem cells using Essential 8 Medium to maintain high-quality feeder-free cell cultures.
Transition pluripotent stem cells from feeder-based or alternative feeder-free systems into Essential 8 Medium with vitronectin matrix.
Compare and select appropriate passaging reagents for different dissociation requirements in pluripotent stem cell culture.
Learn to initiate pluripotent stem cell (PSC) cultures using StemScale PSC Suspension Medium for scalable and efficient expansion in suspension culture.
Maintain and feed pluripotent stem cell cultures in suspension using StemScale PSC Suspension Medium to achieve consistent cell growth.
Passage human pluripotent stem cell cultures grown in suspension with StemScale PSC Suspension Medium for optimal propagation.
Generate up to 20 million neural stem cells from pluripotent stem cells in 7 days using Gibco PSC Neural Induction Medium.
Differentiate pluripotent stem cells into functional beating cardiomyocytes using the Gibco PSC Cardiomyocyte Differentiation Kit.
Induce pluripotent stem cells into definitive endoderm within 48 hours using the Gibco PSC Definitive Endoderm Induction Kit.
For Research Use Only. Not for use in diagnostic procedures.
