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Your journey to stem cell culture excellence begins with Gibco Education. Including both core concepts and laboratory techniques, these handbooks, virtual training modules, and how-to videos can help pave your way to success in PSC (pluripotent stem cell) research.


Virtual stem cell training for PSC researchers

Virtual stem cell training

Each of these in-depth multimedia training modules covers principles and practices of PSC culture and differentiation. You can even test your learning at the end of each module.

Note: You need to be logged in to your Thermo Fisher Scientific on-line account to access the PSC training modules.


How-to videos: Working with pluripotent stem cells in the lab

Expand your knowledge of PSC laboratory techniques with this collection of 3- to 5-minute videos demonstrating procedures such as:

  • preparing media and substrates for PSC culture
  • initiating and adapting PSC cultures
  • passaging, maintenance, and feeding of PSCs
  • inducing and differentiating PSCs

How to prepare StemFlex Medium for PSC culture

This video demonstrates preparation and storage on complete Gibco StemFlex Medium for pluripotent stem cell maintenance.

How to prepare Essential 8 Medium for PSC culture

This video shows how to correctly prepare Essential 8 Medium to support robust, feeder-free pluripotent stem cell culture. Includes important information about thawing and heating.

How to prepare vitronectin substrate plates for PSC culture

Watch and learn how to coat cell culture plates with Vitronectin XF, an extracellular matrix for transferring or expanding pluripotent stem cell colonies.

How to passage PSCs in Essential 8 Medium

Perform routine passaging of pluripotent stem cells using Essential 8 Medium to maintain high-quality feeder-free cell cultures.

How to adapt PSCs from other PSC media into Essential 8 Medium

Transition pluripotent stem cells from feeder-based or alternative feeder-free systems into Essential 8 Medium with vitronectin matrix.

How to select a passaging reagent for PSC culture

Compare and select appropriate passaging reagents for different dissociation requirements in pluripotent stem cell culture.

How to initiate PSC cultures using StemScale PSC Suspension Medium

Learn to initiate pluripotent stem cell (PSC) cultures using StemScale PSC Suspension Medium for scalable and efficient expansion in suspension culture.

How to maintain and feed PSCs using StemScale PSC Suspension Medium

Maintain and feed pluripotent stem cell cultures in suspension using StemScale PSC Suspension Medium to achieve consistent cell growth.

How to passage PSC cultures using StemScale PSC Suspension Medium

Passage human pluripotent stem cell cultures grown in suspension with StemScale PSC Suspension Medium for optimal propagation.

How to generate NSCs from PSCs

Generate up to 20 million neural stem cells from pluripotent stem cells in 7 days using Gibco PSC Neural Induction Medium.

How to differentiate PSCs into cardiomyocytes

Differentiate pluripotent stem cells into functional beating cardiomyocytes using the Gibco PSC Cardiomyocyte Differentiation Kit.

How to induce PSCs into definitive endoderm

Induce pluripotent stem cells into definitive endoderm within 48 hours using the Gibco PSC Definitive Endoderm Induction Kit.


Other tools and resources for stem cell laboratory research

Did you know: You can access free resources to support
every stage of your stem cell research?

Browse our library


For Research Use Only. Not for use in diagnostic procedures.