Stem Cell Research Resource Library

Access our wide selection of tools and resources including application notes, protocols, scientific posters, videos, webinars, and more to support every step of your stem cell research workflow. Choose the tools to best fit your needs.

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Reprogramming
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Cell therapy

These are general resources for all stages of the workflow:

Type	Name
Handbook	Pluripotent Stem Cell Guidebook
Handbook	Pluripotent Stem Cell Resource Handbook
Handbook	Pluripotent Stem Cell Protocol Handbook
Protocols	Stem Cell Protocols
Posters	The Power of Stem Cells
Virtual event	Gibco 24 Days of Stem Cells virtual event
Webinar	24 Days of Stem Cells - Past presentations

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Type	Name
Application note	Improving the stem cell reprogramming characterization workflow: better colony identification using live-cell reagents that enable simultaneous detection of positive and negative pluripotency markers
Application note	Reprogramming of somatic cells to iPSCs using the CytoTune-iPS 2.0 kit with feeder-free media systems
Brochure	Cell reprogramming tools
FAQ	CytoTune-iPS 2.0 Sendai Reprogramming Kit and CytoTune EmGFP Sendai Fluorescence Reporter FAQ
FAQ	Epi5 Episomal iPSC Reprogramming Kit FAQ
FAQ	Episomal iPSC Reprogramming Vectors FAQ
Publication	A systematic evaluation of integration free reprogramming methods for deriving clinically relevant patient specific iPSCs
Publication	Blood cell-derived induced pluripotent stem cells free of reprogramming factors generated by Sendai virus vectors
Publication	Cellular reprogramming of human peripheral blood cells
Publication	Disease-specific induced pluripotent stem cells
Publication	Efficient feeder-free episomal reprograming with small molecules
Publication	Human lymphoblastoid B-cell lines reprogrammed to EBV-free induced pluripotent stem cells
Publication	Induced pluripotent stem cell reprogramming by integration-free sendai virus vectors from peripheral blood of patients with craniometaphyseal dysplasia
Publication	Publications citing the Sendai virus for iPSC generation
Publication	Reprogramming of human somatic cells to pluripotency with defined factors
Publication	Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors
Publication	RNA-based tools for nuclear reprogramming and lineage-conversion: towards clinical applications
Scientific poster	Cell surface marker expression analysis to track progression of fibroblast reprogramming
Scientific poster	CytoTune-iPS 2.0 Sendai Reprogramming Ki for high efficiency reprogramming of fibroblasts and blood-derived cells
Scientific poster	Effect of replacement of c-Myc with L-Myc on Sendai-based reprogramming method
Scientific poster	Efficient generation of induced pluripotent stem cells derived from Parkinson's disease study patient fibroblast lines using CytoTune-iPS 2.0 Reprogramming Kit in the Essential feeder-free media system
Scientific poster	Efficient method to generate vector-free induced pluripotent stem cells from CD34+ and PBMCs using the CytoTune iPS Sendai Reprogramming Kit
Scientific poster	Enabling successful reprogramming of peripheral blood mononuclear cells to induced pluripotent stem cells with CytoTune-iPS 2.0 Sendai Reprogramming Kit
Scientific poster	Essential media system for reprogramming
Scientific poster	Factors that enhance Sendai virus mediated somatic reprogramming
Scientific poster	Generation of zero-footprint iPSCs in xeno-free or feeder-free with CytoTune-iPS Sendai Reprogramming Kit
Scientific poster	Optimization of human CD34+ cells for reprogramming using tailored media and alternate timelines
Scientific poster	Tools that support consistent generation of high quality iPSC using Sendai virus reprogramming for translational research
Training	Gibco PSC Workshop
Video	A Thought Leader's Perspective: Unlocking cardiovascular disease with iPS cells - Dr. Chad Cowan
Video	Advances in Stem Cell Research - Dr. Alexander Meissner
Webinar	Leaving nothing to chance - Dr Mark Tomishima

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Type	Name
Application note	Adaptation of pluripotent stem cells to StemFlex Medium
Application note	CRISPR-Cas9 editing of pluripotent stem cells cultured in StemFlex Medium
Application note	Embryoid body formation in Nunclon Sphera plates
Application note	Genome editing of pluripotent stem cells cultured in StemFlex Medium via electroporation and lipid-based transfection
Application note	Single cell passaging methods
Application note	Superior performance for today’s most challenging stem cell applications: Summary of reprogramming, adaptation, differentiation, and gene editing applications
Brochure	Mesenchymal Stem Cell Resource Guide
Brochure	Essential 8 Media brochure
Brochure	Nunc Cell Culture Imaging Brochure
FAQ	Essential 8 Flex Medium
FAQ	Essential 8 Medium
FAQ	Laminin-521/Adaptation Kit
FAQ	RevitaCell Supplement
Publication	An efficient method for the derivation of mouse embryonic stem cells
Publication	Chemically defined conditions for human iPSC derivation and culture
Publication	Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture
Publication	Common culture conditions for maintenance and cardiomyocyte differentiation of the human embryonic stem cell lines, BG01 and HUES-7
Publication	Cultures of human embryonic stem cells: serum replacement medium or serum-containing media and the effect of basic fibroblast growth factor
Publication	Defined hPSC culture enables highly efficient neuroepithelium derivation without small molecule inhibitors
Publication	Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells
Publication	Derivation of induced pluripotent stem cells from pig somatic cells
Publication	Derivation of neural stem cells from human adult peripheral CD34+ cells for an autologous model of neuroinflammation
Publication	Disease-specific induced pluripotent stem cells
Publication	Efficient and rapid generation of induced pluripotent stem cells using an alternative culture medium
Publication	Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cells
Publication	Efficient iPS cell production with the MyoD transactivation domain in serum-free culture
Publication	Electromechanical integration of cardiomyocytes derived from human embryonic stem cells
Publication	Feeder layer- and serum-free culture of human embryonic stem cells
Publication	Feeder-free growth of undifferentiated human embryonic stem cells
Publication	Generation of germline-competent rat induced pluripotent stem cells
Publication	Generation of GFP reporter human induced pluripotent stem cells using AAVS1 safe harbor Transcription Activator Like Effector Nuclease
Publication	Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts
Publication	Generation of induced pluripotent stem cells from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors
Publication	Generation of induced pluripotent stem cells in the absence of drug selection
Publication	Generation of novel rat and human pluripotent stem cells by reprogramming and chemical approaches
Publication	Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media
Publication	Induction of pluripotent stem cells from adult human fibroblasts by defined factors
Publication	Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds
Publication	Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2
Publication	Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor
Publication	New cell lines from mouse epiblast share defining features with human embryonic stem cells
Publication	Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency
Publication	Optimization of protocols for derivation of mouse embryonic stem cell lines from refractory strains, including the non obese diabetic mouse
Publication	Passaging and colony expansion of hPSCs by enzyme-free dissociation in chemically define culture conditions
Publication	Scalable expansion of human iPSCs in the defined xeno-free E8 medium under adherent and suspension culture conditions
Publication	Serum-based culture conditions provoke gene expression variability in mouse embryonic stem cells as revealed by single-cell analysis
Publication	Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines
Publication	Signaling defects in iPSC-derived fragile X premutation neurons
Publication	Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells
Publication	Unique gene expression signature by human embryonic stem cells cultured under serum-free conditions correlates with their enhanced and prolonged growth in an undifferentiated stage
Scientific poster	A Novel Essential 8 Media System for Robust Weekend-Free Culture of Pluripotent Stem Cells
Scientific poster	Development of a robust, next-generation, feeder-free pluripotent stem cell medium
Scientific poster	Development of feeder-free PSC culture system enabling translational & clinical research
Scientific poster	Development of an improved feeder-free culture system for mouse pluripotent stem cells
Scientific poster	Development of high-quality MSC and NSC for human disease research
Scientific poster	Efficient and scalable GMP-grade culture media system for rapid cardiomyocyte differentiation of pluripotent stem cells in human disease research
Scientific poster	Efficient induction and scale up generation of neural stem cells from human pluripotent stem cells
Scientific poster	Efficient generation and characterization of neural stem cells derived from Parkinson's disease patient and age-matched control induced pluripotent stem cell lines using a novel neural induction method
Scientific poster	Efficient generation of induced pluripotent stem cells derived from Parkinson's disease study patient fibroblast lines using CytoTune-iPS 2.0 Reprogramming Kit in the Essential feeder-free media system
Scientific poster	Efficient iPSC generation from various blood-derived cell types
Scientific poster	Essential 8 Medium: A xeno-free, feeder-free medium for the culture and generation of human iPSCs
Scientific poster	Generation of PSC-derived neural organoids using an alginate-based 3D scaffold
Publication	Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cells
Scientific poster	Generation of zero-footprint iPSCs in xeno-free or feeder-free with CytoTune-iPS Sendai Reprogramming Kit
Scientific poster	Highly efficient and robust generation of neural stem cells from human pluripotent stem cells
Scientific poster	High-quality cGMP-manufactured human mesenchymal stem cell and neural stem cell for human disease research
Scientific poster	High-quality episomal human iPSC derived from cord blood with a zero footprint: Applications in serum-free and xeno-free culture
Scientific poster	Ideal Xeno-free culture conditions for human fibroblasts that facilitate efficient reprogramming
Scientific poster	Impact of passaging method on iPSC quality during early clonal establishment
Scientific poster	Improved cryopreservation and recovery solutions for pluripotent stem cells and difficult-to-preserve primary cells
Scientific poster	Impact of passaging method on iPSC quality during early clonal establishment
Scientific poster	Improved cryopreservation and recovery solutions for pluripotent stem cells and difficult-to-preserve primary cells
Scientific poster	Integrated approach for generation of validated human iPSC banks
Scientific poster	Methods for rapid establishment and scale-up of iPSC
Scientific poster	Modulating growth factor addition to Essential 6 media for a complete xeno-free media from fibroblast culture to iPSC generation and expansion
Scientific poster	Process development strategies to enable large-scale expansion of mesenchymal stem cells for cellular therapy
Scientific poster	Rapid manufacture of custom TAL effector nucleases for genomic editing
Scientific poster	Synthetic surfaces for the culture of human embryonic and adult stem cells
Scientific poster	Using cells from patients with multiple systems atrophy or Parkinson's disease (PARK2, LRRK2, and/or GBA mutations) to build stem cell-derived disease model systems
Scientific poster	Utilizing advanced cryopreservation techniques to produce assay ready primary mouse neurons
Scientific poster	Xeno-free culture systems for pluripotent stem cells
Training	Gibco Education
Training	Gibco PSC Culture Virtual Training Lab
Training	Gibco PSC Workshop
Video	Advances in Stem Cell Research: Essential 8 Medium, A new standard for iPSC cell culture - Dr. Emile Nuwaysir
Video	Building a stem cell matrix - Dr Jeanne Loring
Video	Eyeing the Single Stem Cell - Dr Jeanne Loring
Video	How to adapt pluripotent stem cells from other PSC media into Essential 8 Medium
Video	How to passage PSCs in Essential 8 Medium
Video	How to prepare Essential 8 Medium for PSC Culture
Video	How to prepare Vitronectin substrate plates for use with Essential 8 Medium
Video	Inducing excitement with pluripotency - Dr Jeanne Loring
Video	Stemming the loss of species - Dr Jeanne Loring
Web	Neural cell culture systems
Web	Tools for neural cell culture of primary cells
Video	A Thought Leader's Perspective: Unlocking cardiovascular disease with iPS cells - Dr. Chad Cowan
Video	Advances in Stem Cell Research - Dr. Alexander Meissner
Webinar	Creating integration-free iPSCs and NSCs to Model Parkinson's disease - Dr. Birgitt Schuele
Webinar	Culture & characterization systems for hESCs and iPSCs
Webinar	Culture & characterization systems for mesenchymal stem cells (hMSC)
Webinar	Eliminate serum to gain control of T cell research - New T cell-defined serum replacement
Webinar	Essential 8 & Essential 6 - A feeder-free, xeno-free system for reprogramming and culture of PSCs
Webinar	Essential 8 Flex Medium: Eliminating daily feeding in a feeder-free, xeno-free PSC culture system
Webinar	Generation of transgene-free induced pluripotent stem cells - Dr Uma Lakshmipathy
Webinar	Knockout Serum Replacement – Multi-Species: Feeder-based PSC culture proven more reliable than FBS
Webinar	Leaving nothing to chance - Dr Mark Tomishima
Webinar	Pluripotent Stem Cell Culture Systems: Identification of Appropriate Medium, Matrix, and Passaging Reagent for Your Stem Cell Workflow Needs
Webinar	The evolution of PSC culture media - Dr Rupa Shevde
Webinar	Xeno-free derivation and maintenance of pluripotent cell lines - Dr Jordan Pomeroy

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Type	Name
Application note	CRISPR-Cas9 editing of pluripotent stem cells cultured in StemFlex Medium
Application note	Genome editing of pluripotent stem cells cultured in StemFlex Medium via electroporation and lipid-based transfection
Application note	Lipofectamine 3000 Reagent: Generation and transfection of induced pluripotent stem cells
Application note	Versatility and performance of Lipofectamine Stem Transfection Reagent
Publication	Disease-specific induced pluripotent stem cells
Publication	Expanded activity of dimer nucleases by combining ZFN and TALEN for genome editing
Publication	Induced pluripotent stem cells for neural tissue engineering
Publication	Isolation of single-base genome-edited human iPS cells with antibiotic selection
Publication	RNA-based tools for nuclear reprogramming and lineage-conversion: towards clinical applications
Publication	Unique gene expression signature by human embryonic stem cells cultured under serum-free conditions correlates with their enhanced and prolonged growth in an undifferentiated stage
Publication	Whole-Genome Sequencing Analysis Reveals High Specificity of CRISPR/Cas9 and TALEN-Based Genome Editing in Human iPSCs
Scientific poster	Accelerating genome engineering of induced pluripotent stem cell lines for disease modeling by implementing automated workflows
Scientific poster	Genome Editing of α-Synuclein in iPSCs from a Donor with Multiple System Atrophy
Scientific poster	Genome editing of induced pluripotent stem cells from Parkinson's disease and multiple system atrophy patients
Scientific poster	Lipofectamine 3000: A new transfection reagent for iPSC generation and stem cell genomic engineering
Scientific poster	New tools for improving the genome editing workflow in human induced pluripotent stem cell applications
Scientific poster	Rapid manufacture of custom TAL effector nucleases for genomic editing
Scientific poster	RNA Replicon platform to enable long-lasting transient expression in primary and stem cells
Scientific poster	Use of surrogate reporter vectors to enrich for CRISPR- & TALEN-modified cells
Scientific poster	Using cells from patients with multiple systems atrophy or Parkinson's disease (PARK2, LRRK2, and/or GBA mutations) to build stem cell-derived disease model systems
Training	Gibco Education
Video	A Thought Leader's Perspective: Unlocking cardiovascular disease with iPS cells - Dr. Chad Cowan
Video	Advances in Stem Cell Research - Dr. Alexander Meissner
Webinar	Creating integration-free iPSCs and NSCs to Model Parkinson's disease - Dr. Birgitt Schuele
Webinar	Highly efficient genome editing and cell engineering in stem cells using CRISPR/Cas9
Webinar	Next-generation genome analysis tools for stem cells - Dr Michael Rhodes

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Type	Name
Application note	Differentiation of pluripotent stem cells cultured in StemFlex Medium
Publication	Disease-specific induced pluripotent stem cells
Publication	Human dermal stem cells differentiate into functional epidermal melanocytes
Publication	Improved cell therapy protocols for Parkinson's disease based on differentiation efficiency and safety of hESC-, hiPSC-, and non-human primate iPSC-derived dopaminergic neurons
Publication	Motor Neuron Differentiation from Pluripotent Stem Cells and Other Intermediate Proliferative Precursors that can be Discriminated by Lineage Specific Reporters
Scientific poster	A robust platform for generation of hiPS-derived cardiomyocytes
Scientific poster	Accelerating and synchronizing the differentiation of human pluripotent stem cell-derived neural stem cells into neurons by preventing cell proliferation
Scientific poster	Differentiating human pluripotent stem cells to neurons - Approaches in media development
Scientific poster	Direct derivation and prospective isolation of Schwann cell precursors from human pluripotent stem cells
Scientific poster	Efficient and scalable GMP-grade culture media system for rapid cardiomyocyte differentiation of pluripotent stem cells in human disease research
Scientific poster	Generation of large number of floor plate derived, midbrain-specified DA neurons from human PSCs for scaled application
Scientific poster	Novel neural induction method for efficient generation of neural stem cells derived from Parkinson's
Scientific poster	Single-cell analysis of transcription factor expression during differentiation of PSC to cardiomyocytes using flow cytometry
Scientific poster	Xenofree Culture Systems for Stem Cells
Video	A Thought Leader's Perspective: Unlocking cardiovascular disease with iPS cells - Dr. Chad Cowan
Video	Advances in Stem Cell Research - Dr. Alexander Meissner
Video	How to differentiate pluripotent stem cells into cardiomyocytes
Video	How to induce pluripotent stem cells in to definitive endoderm
Video	How to use Gibco PSC Neural Induction Medium
Webinar	Differentiation of Midbrain Floor Plate Progenitors and Dopaminergic Neurons from Human Pluripotent Stem Cells
Webinar	microRNA and epigenetic regulation of neural stem cell differentiations - Dr Ron Hart
Webinar	Neurons from Human Pluripotent Stem Cells
Webinar	PSCs to cardiomyocytes in three steps
Webinar	Rapid induction of neural stem cells without the need for EB formation
White paper	B-27 Plus Neuronal Culture System enables superior primary neuron cultures
White paper	Disease modeling of hypertrophic cardiomyopathy from patients carrying sarcomeric mutations

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Type	Name
Application note	Improving the stem cell characterization workflow: Confirming differentiation potential via multi-color detection of germ layer markers
Application note	Improving the stem cell reprogramming characterization workflow: better colony identification using live-cell reagents that enable simultaneous detection of positive and negative pluripotency markers
Brochure	PSC characterization solutions
Brochure	TaqMan hPSC Scorecard Panel and Software
FAQ	TaqMan hPSC Scorecard Panel FAQ
Handbook	Antibodies for Stem Cell Research Handbook
Handbook	Gibco Neurobiology Protocol Handbook
Publication	A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells
Publication	Characterization of human Huntington's Disease cell model from induced pluripotent stem cells
Publication	Disease-specific induced pluripotent stem cells
Publication	Signaling defects in iPSC-derived fragile X premutation neurons
Scientific poster	Antibody-based tools and protocols for improving stem cell characterization workflow
Scientific poster	Characterization of human limbal stem cells using acoustic focusing cytometry
Scientific poster	Efficient generation and characterization of neural stem cells derived from Parkinson's disease patient and age-matched control induced pluripotent stem cell lines using a novel neural induction method
Scientific poster	Molecular analysis tools for epigenomic characterization of pluripotent stem cells
Scientific poster	Novel Alkaline Phosphatase Live Stain to Identify and Isolate Emerging iPSCs
Scientific poster	Novel markers to distinguish pluripotent and non-pluripotent cells
Scientific poster	TaqMan Assays for rapid assessment of genome, epigenome, and quality of integration free induced pluripotent stem cells
Scientific poster	Utility of hPSC Scorecard Assay in assessment of functional pluripotency of cells across the iPSC workflow
Training	Gibco PSC Workshop
Video	A Thought Leader's Perspective: Unlocking cardiovascular disease with iPS cells - Dr. Chad Cowan
Video	Advances in Stem Cell Research - Dr. Alexander Meissner
Video	Alkaline Phosphatase Live Stain
Video	How to Use the TaqMan hPSC Scorecard Panel
Video	Why we chose the TaqMan hPSC Scorecard Assay - Jennifer Moore, RUCDR/Infinite Biologics
Webinar	Antibody-based tools and protocols for characterization of pluripotent stem cells
Webinar	Characterization of human pluripotent stem cells - Dr. Alexander Meissner
Webinar	Culture & characterization systems for hESCs and iPSCs
Webinar	Culture & characterization systems for mesenchymal stem cells (hMSC)
Webinar	Identifying iPSCs with a novel Live Alkaline Phosphatase Stain
Webinar	Pluripotent stem cell characterization methods

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Type	Name
Brochure	Mesenchymal Stem Cell Resource Guide
Brochure	Cell Therapy Systems brochure
FAQ	Cell Therapy Systems
Publication	Disease-specific induced pluripotent stem cells
Publication	Improved cell therapy protocols for Parkinson's disease based on differentiation efficiency and safety of hESC-, hiPSC-, and non-human primate iPSC-derived dopaminergic neurons
Scientific poster	Development of feeder-free PSC culture system enabling translational & clinical research
Scientific poster	Tools that support consistent generation of high quality iPSC using Sendai virus reprogramming for translational research
Scientific poster	Cell Therapy Systems (CTS) Sendai virus reprogramming kit for xeno-free generation of iPSCs
Scientific poster	Enabling efficient ex vivo expansion of long-term human hematopoietic stem and progenitor cells For cell therapy
Scientific poster	Clinical-grade expansion of human mesenchymal stem cells using a microcarrier-based culture system under serum-free and xeno-free conditions
Video	CTS Mini-Documentary Series
Video	Barriers in stem cell therapy - Dr Jeanne Loring
Video	A Thought Leader's Perspective: Unlocking cardiovascular disease with iPS cells - Dr. Chad Cowan
Video	Advances in Stem Cell Research - Dr. Alexander Meissner
Webinar	Improved T cell function and in vivo engraftment of CAR-T cells expanded ex vivo with CTS Immune Cell SR
Webinar	MSC Culture & Beyond
Webinar	MSC Culture Systems & Applications
Webinar	Thermo Fisher Scientific's cell therapy capabilities
Webinar	Eliminate serum to gain control of T cell research - New T cell-defined serum replacement