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Pluripotent Stem Cell Resource Handbook

Whether you’re new to the field or expanding into new areas, this educational handbook will help guide your pluripotent stem cell research. It offers the latest tools, resources, applications, and more, to support every step of your PSC workflow.

24 Hours of Stem Cells virtual event

Register to attend this year’s 24 Hours of Stem Cells virtual event on
November 15, 2018 where you can access presentations, virtual training, certifications, resources and more.

Gibco Pluripotent Stem Cell Workshop

Get hands-on stem cell training in techniques for culturing and characterizing human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), as well as reprogramming techniques for the creation of iPSCs at one of our research centers.

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Type Name
Application note Improving the stem cell characterization workflow: better colony identification using live-cell reagents that enable simultaneous detection of positive and negative pluripotency markers 
Application note Lipofectamine 3000 Reagent: Generation and transfection of induced pluripotent stem cells
Application note Application Note: Embryoid body formation in Nunclon Sphera plates
Application note Single cell passaging methods
FAQ Cell Therapy Systems
FAQ CytoTune-iPS 2.0 Sendai Reprogramming Kit and CytoTune EmGFP Sendai Fluorescence Reporter FAQ
FAQ Epi5 Episomal iPSC Reprogramming Kit FAQ
FAQ Episomal iPSC Reprogramming Vectors FAQ
FAQ Essential 8 Flex Medium
FAQ Essential 8 Medium
FAQ Essential 8 Medium and Vitronectin
FAQ Laminin-521/Adaptation Kit
FAQ RevitaCell Supplement
FAQ TaqMan hPSC Scorecard Panel FAQ
Handbook Antibodies for Stem Cell Research Handbook
Handbook Gibco Neurobiology Protocol Handbook
Handbook Pluripotent Stem Cell Guidebook
Handbook Pluripotent Stem Cell Resource Handbook
Handbook Pluripotent Stem Cell Protocol Handbook
Literature Cell reprogramming tools
Literature Cell Therapy Systems brochure
Literature Essential 8 Media brochure
Literature Improving the stem cell characterization workflow: Confirming differentiation potential via multi-color detection of germ layer markers
Literature Nunc Cell Culture Imaging Brochure
Literature TaqMan hPSC Scorecard Panel and Software
Newsletter Stem Cell Intel
Protocol 3-Germ Layer Immunocytochemistry Kit
Protocol A introduction to stem cell maintenance
Protocol Adaptation of hPSCs from different culture systems to Essential 8 Medium or Essential 8 Flex Medium
Protocol Creation of embryoid bodies from iPSCs using Complete KnockOut Serum Replacement Feeder-Free Medium 
Protocol Culturing human embryonic stem cells on MEF-conditioned medium 
Protocol Culturing induced pluripotent stem cells (iPSCs) on MEF-conditioned medium
Protocol Culturing PSCs in Essential 8 Medium
Protocol CytoTune EmGFP Sendai Fluorescence Reporter
Protocol CytoTune-iPS 2.0 Sendai Reprogramming Kit
Protocol Derivation of dopaminergic neurons from human embryonic stem cells
Protocol Detecting pluripotency with Alkaline Phosphatase Live Stain
Protocol Differentiating glial precursor cells into astrocytes and oligodendrocytes
Protocol Differentiating neural stem cells into neurons and glial cells
Protocol Enrichment and replating of PSC-derived cardiomyocytes
Protocol Epi5 Episomal iPSC Reprogramming Kit
Protocol Episomal iPSC Reprogramming Vectors
Protocol Essential 6 Medium
Protocol Essential 8 Adaptation Kit
Protocol Essential 8 Flex Medium
Protocol Essential 8 Medium
Protocol Feeder-dependent culture and passaging mouse embryonic stem cells (mESCs) in KnockOut Serum Replacement with leukemia inhibitory factor (LIF)
Protocol Feeder-dependent culture of human embryonic stem cells (hESCs)
Protocol Feeder-dependent culture of human induced pluripotent stem cells (hiPSCs)
Protocol Formation of embryoid bodies (EBs) from mouse embryonic stem cells (mESCs)
Protocol Geltrex LDEV-Free, hESC-qualified, Reduced Growth Factor Basement Membrane Matrix
Protocol General Stem Cell Protocols
Protocol Generation of Human Induced Pluripotent Stem Cells (hiPSC) from Fibroblasts using Episomal Vectors
Protocol Gibco Episomal hiPSC Line
Protocol hPSC Scorecard Analysis Software
Protocol Human Cardiomyocyte Immunocytochemistry Kit
Protocol Human Dopaminergic Neuron Immunocytochemistry Kit
Protocol Human Neural Stem Cell Immunocytochemistry Kit
Protocol Induction of neural stem cells from human pluripotent stem cells using Gibco PSC Neural Induction Medium
Protocol Mouse (ICR) Inactivated Embryonic Fibroblasts
Protocol Neural Stem Cell Protocols
Protocol Pluripotent Stem Cell Protocols
Protocol PSC 4-Marker Immunocytochemistry Kit (OCT4, SSEA4, SOX2, TRA-1-60)
Protocol PSC Cardiomyocyte Differentation Kit
Protocol PSC Cardiomyocyte Differentation Kit quick reference guide
Protocol PSC Definitive Endoderm Induction Kit
Protocol PSC Definitive Endoderm Induction Kit quick reference guide
Protocol PSC Dopaminergic Neuron Differentiation Kit (prototype)
Protocol PSC Neural Induction Medium
Protocol PSC Neural Induction Medium quick reference guide
Protocol Reprogramming peripheral blood mononuclear cells (PBMCs) with the CytoTune-iPS 2.0 Reprogramming Kit under feeder-free conditions
Protocol RevitaCell Supplement
Protocol Single-cell dissociation of PSCs for cardiomyocyte differentiation
Protocol StemPro hESC SFM
Protocol TaqMan hPSC Scorecard Panel Quick Reference Card
Protocol Using the TaqMan hPSC Scorecard Panel
Protocol Vitronectin, truncated recombinant human (VTN-N)
Publication A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells
Publication A systematic evaluation of integration free reprogramming methods for deriving clinically relevant patient specific iPSCs
Publication An efficient method for the derivation of mouse embryonic stem cells
Publication Blood cell-derived induced pluripotent stem cells free of reprogramming factors generated by Sendai virus vectors
Publication Cellular reprogramming of human peripheral blood cells
Publication Characterization of human Huntington's Disease cell model from induced pluripotent stem cells
Publication Chemically defined conditions for human iPSC derivation and culture
Publication Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture
Publication Common culture conditions for maintenance and cardiomyocyte differentiation of the human embryonic stem cell lines, BG01 and HUES-7
Publication Cultures of human embryonic stem cells: serum replacement medium or serum-containing media and the effect of basic fibroblast growth factor
Publication Defined hPSC culture enables highly efficient neuroepithelium derivation without small molecule inhibitors
Publication Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells
Publication Derivation of induced pluripotent stem cells from pig somatic cells
Publication Derivation of neural stem cells from human adult peripheral CD34+ cells for an autologous model of neuroinflammation
Publication Disease-specific induced pluripotent stem cells
Publication Efficient and rapid generation of induced pluripotent stem cells using an alternative culture medium
Publication Efficient feeder-free episomal reprograming with small molecules
Publication Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord bloodmononuclear cells
Publication Efficient iPS cell production with the MyoD transactivation domain in serum-free culture
Publication Electromechanical integration of cardiomyocytes derived from human embryonic stem cells
Publication Expanded activity of dimer nucleases by combining ZFN and TALEN for genome editing
Publication Feeder layer- and serum-free culture of human embryonic stem cells
Publication Feeder-free growth of undifferentiated human embryonic stem cells
Publication Generation of germline-competent rat induced pluripotent stem cells
Publication Generation of GFP reporter human induced pluripotent stem cells using AAVS1 safe harbor Transicription Activator Like Effector Nuclease
Publication Generation of induced pluripotent stem cells from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors
Publication Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts
Publication Generation of induced pluripotent stem cells in the absence of drug selection
Publication Generation of novel rat and human pluripotent stem cells by reprogramming and chemical approaches
Publication Human dermal stem cells differentiate into functional epidermal melanocytes
Publication Human lymphoblastoid B-cell lines reprogrammed to EBV-free induced pluripotent stem cells
Publication Improved cell therapy protocols for Parkinson's disease based on differentiation efficiency and safety of hESC-, hiPSC-, and non-human primate iPSC-derived dopaminergic neurons
Publication Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media
Publication Induced pluripotent stem cell reprogramming by integration-free sendai virus vectors from peripheral blood of patients with craniometaphyseal dysplasia
Publication Induced pluripotent stem cells for neural tissue engineering
Publication Induction of pluripotent stem cells from adult human fibroblasts by defined factors
Publication Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds
Publication Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2
Publication Isolation of single-base genome-edited human iPS cells with antiobiotic selection
Publication Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor
Publication Motor Neuron Differentiation from Pluripotent Stem Cells and Other Intermediate Proliferative Precursors that can be Discriminated by Lineage Specific Reporters
Publication New cell lines from mouse epiblast share defining features with human embryonic stem cells
Publication Neural Cell Culture Publications Finder
Publication Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency
Publication Optimization of protocols for derivation of mouse embryonic stem cell lines from refractory strains, including the non obese diabetic mouse
Publication Passaging and colony expansion of hPSCs by enzyme-free dissocation in chemically define culture conditions
Publication Publications citing the Sendai virus for iPSC generation
Publication Reprogramming of human somatic cells to pluripotency with defined factors
Publication Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors
Publication RNA-based tools for nuclear reprogramming and lineage-conversion: towards clinical applications
Publication Scalable expansion of human iPSCs in the defined xeno-free E8 medium under adherent and suspension culture conditions
Publication Serum-based culture conditions provoke gene expression variability in mouse embryonic stem cells as revealed by single-cell analysis
Publication Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines
Publication Signaling defects in iPSC-derived fragile X premutation neurons
Publication Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells
Publication Unique gene expression signature by human embryonic stem cells cultured under serum-free conditions correlates with their enhanced and prolonged growth in an undifferentiated stage
Publication Whole-Genome Sequencing Analysis Reveals High Specificity of CRISPR/Cas9 and TALEN-Based Genome Editing in Human iPSCs
Poster The Power of Stem Cells
Scientific poster ISSCR 2017: Accelerating and synchronizing the differentiation of human pluripotent stem cell-derived neural stem cells into neurons by preventing cell proliferation
Scientific poster ISSCR 2017: Cell Therapy Systems (CTS) Sendai virus reprogramming kit for xeno-free generation of iPSCs
Scientific poster ISSCR 2017: Development of a robust, next-generation, feeder-free pluripotent stem cell medium
Scientific poster ISSCR 2017: Development of an improved feeder-free culture system for mouse pluripotent stem cells
Scientific poster ISSCR 2017: Effect of replacement of c-Myc with L-Myc on Sendai-based reprogramming method
Scientific poster ISSCR 2017: Impact of passaging method on iPSC quality during early clonal establishment
Scientific poster ISSCR 2017: New tools for improving the genome editing workflow in human induced pluripotent stem cell applications
Scientific poster ISSCR 2017: RNA Replicon platform to enable long-lasting transient expression in primary and stem cells
Scientific poster ISSCR 2017: Single-cell analysis of transcription factor expression during differentiation of PSC to cardiomyocytes using flow cytometry
Scientific poster A Novel Essential 8 Media System for Robust Weekend-Free Culture of Pluripotent Stem Cells
Scientific poster Antibody-based tools and protocols for improving stem cell characterization workflow
Scientific poster A robust platform for generation of hiPS-derived cardiomyocytes
Scientific poster Cell surface marker expression analysis to track progression of fibroblast reprogramming
Scientific poster Characterization of human limbal stem cells using acoustic focusing cytometry
Scientific poster Clinical-grade expansion of human mesenchymal stem cells using a microcarrier-based culture system under serum-free and xeno-free conditions
Scientific poster CytoTune-iPS 2.0 Sendai Reprogramming Ki for high efficiency reprogramming of fibroblasts and blood-derived cells
Scientific poster Development of feeder-free PSC culture system enabling translational & clinical research
Scientific poster Development of high-quality MSC and NSC for human disease research
Scientific poster Differentiating human pluripotent stem cells to neurons - Approaches in media development
  Direct derivation and prospective isolation of Schwann cell precursors from human pluripotent stem cells
Scientific poster Effect of DGCR8 knockdown on human embryonic and adult stem cells
Scientific poster Efficient and scalable GMP-grade culture media system for rapid cardiomyocyte differentiation of pluripotent stem cells in human disease research
Scientific poster Efficient generation and characterization of neural stem cells derived from Parkinson's disease patient and age-matched control induced pluripotent stem cell lines using a novel neural induction method
Scientific poster Efficient generation of induced pluripotent stem cells derived from Parkinson's disease study patient fibroblast lines using CytoTune-iPS 2.0 Reprogramming Kit in the Essential feeder-free media system
Scientific poster Efficient induction and scale up generation of neural stem cells from human pluripotent stem cells
Scientific poster Efficient iPSC generation from various blood-derived cell types
Scientific poster Efficient method to generate vector-free induced pluripotent stem cells from CD34+ and PBMCs using the CytoTune iPS Sendai Reprogramming Kit
Scientific poster Enabling successful reprogramming of peripheral blood mononuclear cells to induced pluripotent stem cells with CytoTune-iPS 2.0 Sendai Reprogramming Kit
Scientific poster Essential 8 Medium: A xeno-free, feeder-free medium for the culture and generation of human iPSCs
Scientific poster Essential media system for reprogramming
Scientific poster Factors that enhance Sendai virus mediated somatic reprogramming
Scientific poster Generation of large number of floor plate derived, midbrain-specified DA neurons from human PSCs for scaled application
Scientific poster Generation of transgene-free iPSCs From Parkinson's disease patients
Scientific poster Generation of zero-footprint iPSCs in xeno-free or feeder-free with CytoTune-iPS Sendai Reprogramming Kit
Scientific poster Genome Editing of α-Synuclein in iPSCs from a Donor with Multiple System Atrophy
Scientific poster Genome editing of induced pluripotent stem cells from Parkinson's disease and multiple system atrophy patients
Scientific poster High-quality cGMP-manufactured human mesenchymal stem cell and neural stem cell for human disease research
Scientific poster High-quality episomal human iPSC derived from cord blood with a zero footprint: Applications in serum-free and xeno-free culture
Scientific poster Highly efficient and robust generation of neural stem cells from human pluripotent stem cells
Scientific poster Human adipose-derived stem cell characterization and comparative gene expression profiling
Scientific poster Ideal Xeno-free culture conditions for human fibroblasts that facilitate efficient reprogramming
Scientific poster Improved cryopreservation and recovery solutions for pluripotent stem cells and difficult-to-preserve primary cells
Scientific poster Integerated approach for generation of validated human iPSC banks
Scientific poster Integrated platform for creation of assay-ready neural cells
Scientific poster Large-scale and focused transcriptome analysis of integration-free induced pluripotent stem cells
Scientific poster Lipofectamine 3000: A new transfection reagent for iPSC generation and stem cell genomic engineering
Scientific poster Modulating growth factor addition to Essential 6 media for a complete xeno-free media from fibroblast culture to iPSC generation and expansion
Scientific poster Molecular analysis tools for epigenomic characterization of pluripotent stem cells
Scientific poster Novel Alkaline Phosphatase Live Stain to Identify and Isolate Emerging iPSCs
Scientific poster Novel markers to distinguish pluripotent and non-pluripotent cells
Scientific poster Novel neural induction method for efficient generation of neural stem cells dervied from Parkinson's
Scientific poster Optimization of human CD34+ cells for reprogramming using tailored media and alternate timelines
Scientific poster Optimized neuronal culture media for electrophysiology
Scientific poster Process development strategies to enable large-scale expansion of mesenchymal stem cells for cellular therapy
Scientific poster Quantitation of rare undifferentiated hESCs by methylation-specific CastPCR (MeS castPCR)
Scientific poster Rapid manufacture of custom TAL effector nucleases for genomic editing
Scientific poster Recombinant human Laminin-521 supports PSC survival in Essential 8 medium during critical transitions
Scientific poster Synthetic surfaces for the culture of human embryonic and adult stem cells
Scientific poster Synthetic surfaces for the culture of human embryonic and adult stem cells
Scientific poster TaqMan Assays for rapid assessment of genome, epigenome, and quality of integration free induced pluripotent stem cells
Scientific poster Use of surrogate reporter vectors to enrich for CRISPR- & TALEN-modified cells
Scientific poster Using cells from patients with multiple systems atrophy or Parkinson's disease (PARK2, LRRK2, and/or GBA mutations) to build stem cell-derived disease model systems
Scientific poster Utility of hPSC Scorecard Assay in assessment of functional pluripotency of cells across the iPSC workflow
Scientific poster Utilizing advanced cryopreservation techniques to produce assay ready primary mouse neurons
Scientific poster Xeno-free culture systems for pluripotent stem cells
Scientific poster Xeno-free culture systems for stem cells
Selection guide Mesenchymal Stem Cell Resource Guide
Selection guide Tools for neural cell culture of primary cells
Selection guide Neural cell culture systems
Selection guide Pluripotent Stem Cell Guidebook
Training Gibco PSC Culture Virtual Training Lab
Training Gibco PSC Workshop
Video A Thought Leader's Perspective: Unlocking cardiovascular disease with iPS cells - Dr. Chad Cowan
Video Advances in Stem Cell Research - Dr. Alexander Meissner
Video Advances in Stem Cell Research: Essential 8 Medium, A new standard for iPSC cell culture - Dr. Emile Nuwaysir
Video Alkaline Phosphatase Live Stain
Video CTS Mini-Documentary Series
Video How to adapt pluripotent stem cells from other PSC media into Essential 8 Medium
Video How to differentiate pluripotent stem cells into cardiomyocytes
Video How to induce pluripotent stem cells in to definitive endoderm
Video How to passage PSCs in Essential 8 Medium
Video How to prepare Essential 8 Medium for PSC Culture
Video How to prepare Vitronectin substrate plates for use with Essential 8 Medium
Video How to use Gibco PSC Neural Induction Medium
Video How to Use the TaqMan hPSC Scorecard Panel
Video Why we chose the TaqMan hPSC Scorecard Assay - Jennifer Moore, RUCDR/Infinite Biologics
Virtual event Gibco 24 Hours of Stem Cells virtual event
Web PSC Dopaminergic Neuron Differentiation Kit (prototype) landing page
Webinar 24 Hours of Stem Cells - On-demand presentations 2014/2015
Webinar Antibody-based tools and protocols for characterization of pluripontent stem cells
Webinar Antibodies for stem cell research
Webinar Barriers in stem cell therapy - Dr Jeanne Loring
Webinar Building a stem cell matrix - Dr Jeanne Loring
Webinar Characterization of human pluripotent stem cells - Dr. Alexander Meissner
Webinar Considerations for the culture of pluripotent stem cells
Webinar Creating integration-free iPSCs and NSCs to Model Parkinson's disease - Dr. Birgitt Schuele
Webinar Culture adaptation of pluripotent stem cells: an in vitro model for oncogensis
Webinar Culture & characterization systems for hESCs and iPSCs
Webinar Culture & characterization systems for mesenchymal stem cells (hMSC)
Webinar Development of chemically defined cell culture for human pluripotent stem cells
Webinar Differentiation of Midbrain Floor Plate Progenitors and Dopaminergic Neurons from Human Pluripotent Stem Cells
Webinar Eliminate serum to gain control of T cell research - New T cell-defined serum replacement
Webinar Epigenetic regulation in stem cells and reprogramming
Webinar Essential 8 & Essential 6 - A feeder-free, xeno-free system for reprogramming and culture of PSCs
Webinar Essential 8 Flex Medium: Eliminating daily feeding in a feeder-free, xeno-free PSC culture system
Webinar Eyeing the Single Stem Cell - Dr Jeanne Loring
Webinar Generation of transgene-free induced pluripotent stem cells - Dr Uma Lakshmipathy
Webinar Genome editing and stem cell engineering for disease modeling
Webinar Highly efficient genome editing and cell engineering in stem cells using CRISPR/Cas9
Webinar Human neural stem cell (hNSC) platforms
Webinar Improved T cell function and in vivo engraftment of CAR-T cells expanded ex vivo with CTS Immune Cell SR
Webinar Inducing excitement with pluripotency - Dr Jeanne Loring
Webinar Identifying iPSCs with a novel Live Alkaline Phosphatase Stain
Webinar Knockout Serum Replacement – Multi-Species: Feeder-based PSC culture proven more reliable than FBS
Webinar Leaving nothing to chance - Dr Mark Tomishima
Webinar mircoRNA and epigenetic regulation of neural stem cell differentiations - Dr Ron Hart
Webinar MSC Culture & Beyond
Webinar MSC Culture Systems & Applications
Webinar Molecular engineering of biomaterial platforms for scalable stem cell manufacturing
Webinar Next-generation genome analysis tools for stem cells - Dr Michael Rhodes
Webinar Pluripotent stem cell characterization methods
Webinar Pluripotent Stem Cell Culture Systems: Identification of Appropriate Medium, Matrix, and Passaging Reagent for Your Stem Cell Workflow Needs
Webinar PSCs to cardiomyocytes in three steps
Webinar Rapid induction of neural stem cells without the need for EB formation
Webinar Reprogramming cells to study mental disorders
Webinar Stem cells as a model for morphogenesis of tissues - Dr Elaine Fuchs
Webinar Stemming the loss of species - Dr Jeanne Loring
Webinar The evolution of PSC culture media - Dr Rupa Shevde
Webinar The future of pluripotent stem cell banking
Webinar Thermo Fisher Scientific's cell therapy capabilities
Webinar Utility of gene expression signatures for the characterization of cellular identity
Webinar Xeno-free derivation and maintenance of pluripotent cell lines - Dr Jordan Pomeroy
White paper Disease modeling of hypertrophic cardiomyopathy from patients carrying sarcomeric mutations

For Research Use Only. Not for use in diagnostic procedures.