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Advancing your pluripotent stem cell therapy research to clinical applications requires careful material selection as the quality of starting materials significantly impacts the properties of your final stem cell therapy product.

At Thermo Fisher Scientific, we have developed Gibco Cell Therapy Systems (CTS) products across the pluripotent stem cell therapy workflow to ease the transition from discovery to commercial manufacturing. We offer high quality GMP manufactured, commercial scale ancillary materials with a high degree of qualification, traceability, and regulatory documentation.

  • Scalable manufacturing under GMP conditions - Gibco media and supplements are manufactured in conformity with Good Manufacturing Practices (GMP) for medical devices, 21 CFR Part 820, and follow USP<1043> and Ph Eur 5.2.12, and our manufacturing sites are FDA-registered and ISO 13485- and/or ISO 9001-certified. Our investment in rigorous product manufacturing ensures the consistency, reliability, and high quality of PSC therapy reagents in combination with the ability to scale up and accommodate changing needs.
  • Quality control testing and regulatory documentation - CTS media and reagents help minimize the risk of contamination and variability in your research with extensive safety testing, including sterility, endotoxin and mycoplasma. Full traceability for raw materials is also available, including Drug Master Files and/or Regulatory Support Files, and certificates of origin.
  • Proven use for a seamless transition from research to clinic - We offer an extensive selection of serum-free, xeno-free, or animal origin–free formulations with cell therapy specific intended use statements and complementary research use reagents. Our CTS products have been used in over 200 clinical trials as well as in commercially approved cell therapies including FDA-approved CAR-T therapies and the first FDA-approved therapeutic cancer vaccine.

Gibco CTS Rotea Counterflow Centrifugation System

Gibco CTS Rotea Counterflow Centrifugation System

A compact, flexible closed cell processing system, designed to streamline and expedite your cell therapy development, that enables:

  • Process flexibility—user-programmable software enables you to create and optimize a broad range of protocols for cell separation, washing, and concentration
  • High cell recovery and viability—gentle processing enables >95% cell recovery while maintaining cell viability
  • Low output volumes—proprietary technology can deliver as little as 5 mL of concentrate
  • Research through commercial manufacturing—the closed single-use kit enables sterile processing, and an OPC-UA interface enables connectivity to a 21 CFR Part 11-compliant system

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CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit

Gibco CTS CytoTune iPS 2.1 Sendai Reprogramming Kit

The first off-the-shelf reprogramming system manufactured in accordance with GMP requirements, offering high-efficiency Sendai delivery of reprogramming factors.

  • Xeno-free workflow—generate iPSC lines from both fibroblasts and blood for clinical research
  • Regulatory support—traceability documentation, such as Certificate of Analysis and Certificate of Origin, available to support your regulatory filing
  • Designed with transition in mind—complimentary research-use CytoTune iPS 2.0 Sendai Reprogramming Kit helps facilitate a seamless translation to the clinic

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CTS Essential 8 Medium

Gibco CTS Essential 8 Medium

Based on the widely cited Essential 8 Medium, Gibco CTS Essential 8 Medium is the first globally available human pluripotent stem cell (hPSC) culture medium with components not directly derived from animals.

  • Supports long-term iPSC culture—maintains iPSC marker expression and a normal karyotype over multiple passages
  • Maintains differentiation potential—PSCs cultured in CTS Essential 8 Medium are able to generate cells of all three germ layers
  • Helps reduce risk—based on the same fully defined and highly consistent eight-component formulation as RUO Essential 8 Medium but without components directly of animal or human origin

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CTS Essential 6 Medium

Gibco CTS Essential 6 Medium

Gibco CTS Essential 6 Medium is a xeno-free, feeder-free medium which supports the spontaneous or directed differentiation of human pluripotent stem cells (hPSCs) and the reprogramming of somatic cells.

  • Provides a flexible format—suits different applications and various cell types
  • Simplifies the transition from bench to clinic—contains the same six essential components as RUO Essential 6 Medium, and with its cGMP manufacturing process, this formulation helps minimize variability
  • Supports differentiation and reprogramming—does not contain bFGF or TGFbeta

CTS Vitronectin (VTN-N) Recombinant Human Protein

Gibco CTS Vitronectin (VTN-N) Recombinant Human Protein

Designed in the laboratory of James Thomson, Gibco CTS Vitronectin (VTN-N) Recombinant Human Protein is a defined matrix for feeder-free culture of iPSCs and is intended for use with the CTS Essential 8 culture system.

  • Animal origin–free—microbial expression recombinant matrix helps reduce variability and viral contamination risk in iPSC cultures
  • Optimized and defined surface—VTN-N variant has been shown to support human iPSC attachment and survival better than wild type vitronectin
  • Supports pluripotency—able to maintain pluripotency, normal growth characteristics, trilineage differentiation potential and normal karyotype after extended culture


CTS RevitaCell Supplement

Plastic 5 mL vial containing clear CTS RevitaCell Supplement

Gibco CTS RevitaCell Supplement (100X) is an animal-origin-free, chemically defined supplement used with PSCs for post-thaw recovery or in combination with CTS Essential 8 Medium for single cell passaging. 

  • Improved cell survival—provides high cell viability post-single cell passaging and post-thaw
  • Contains higher specificity inhibitor—includes a higher specificity ROCK inhibitor, coupled with antioxidants and free radical scavengers to minimize impact of stress on cells
  • Offers consistency—formulated using animal-origin free and chemically defined materials

CTS Versene Solution

Plastic 100 mL supplement bottle containing clear CTS Versene

Gibco CTS Versene is a gentle non-enzymatic cell dissociation reagent for routine clump passaging of PSCs while maintaining viability over multiple passages.

  • Maintain viability over multiple passages—PSCs passaged with this cGMP manufactured EDTA dissociation reagent maintain viability
  • Seamless transition from bench to clinic—same formulation as RUO Versene Solution but with additional characterization and quality control testing

CTS PSC Cryopreservation Kit

Plastic 5 mL vial containing clear CTS RevitaCell Supplement accompanied by a plastic 50 mL bottle containing red-colored CTS PSC Cryomedium

The Gibco CTS PSC Cryopreservation Kit, which includes CTS PSC Cryomedium and CTS RevitaCell Supplement, minimizes the loss of cell viability and maximizes post-thaw recovery of PSCs.

  • Provides efficient recovery—routinely achieves higher viability than traditional methods of PSC cryopreservation
  • Offers consistency—chemically defined media maintains normal morphology, pluripotency, and karyotype of PSCs
  • Reduces variability—optimized reagents minimize the causes of genetic instability of PSCs

CTS PSC Cryomedium

Plastic 50 mL bottle containing red-colored CTS PSC Cryomedium

Gibco CTS PSC Cryomedium is a xeno-free solution for the cryopreservation of PSCs. It also helps minimize loss of cell viability and maximize post-thaw recovery as part of the CTS PSC Cryopreservation Kit.

  • Delivers higher recovery efficiency—efficient recovery and higher viability than traditional methods of cryopreservation
  • Provides versatility—compatible with clump and singe cell passaged PSCs cultured in a variety of media systems
  • Offers ease of use—provided as a ready-to-use , complete solution with no required additions of enhancing reagents

CTS KnockOut SR XenoFree Medium

Gibco CTS KnockOut SR XenoFree Medium

Gibco CTS KnockOut SR XenoFree Medium is a defined, xeno-free serum replacement based on the traditional KnockOut Serum Replacement, which has been cited in more than 2,000 publications and trusted for over 20 years.

  • Supports pluripotency—able to maintain the pluripotency, normal morphology, and karyotype of hPSCs
  • Versatile—can also be used for cryopreservation, derivation, and differentiation of iPSCs
  • Designed for your applications—a first-to-market, xeno-free iPSC product

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CTS KnockOut DMEM and CTS KnockOut DMEM/F12

Gibco CTS KnockOut DMEM and Gibco CTS KnockOut DMEM/F12

Your choice of chemically defined human- and animal origin-free basal media for pluripotent stem cell culture. Based on traditional DMEM and DMEM/F12 formulations, these basal media are:

  • Optimized—lower osmolarity as compared to traditional formulations improve maintenance of undifferentiated iPSCs
  • Versatile—have been shown to support expansion and differentiation of a variety of stem cells, including iPSCs
  • Tried and tested—cited in many publications, often used with CTS KnockOut SR XenoFree supplement

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Intended use of the products mentioned on this page vary. For specific intended use statements please refer to the product label.