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Advance to clinical applications with CTS iPSC media and reagents

Advancing induced pluripotent stem cell (iPSC) therapy research to clinical applications requires researchers to carefully select materials, as the quality of starting materials has a significant impact on the properties of the final stem cell therapy product.

Thermo Fisher Scientific has developed Gibco Cell Therapy Systems (CTS) products throughout the pluripotent stem cell therapy workflow, aiming to help facilitate the transition from discovery to commercial manufacturing. The company provides high-quality ancillary materials manufactured under Good Manufacturing Practices (GMP), helping ensure commercial scale production. These materials are extensively qualified, traceable, and accompanied by regulatory documentation.

  • Scalable manufacturing under GMP conditions—CTS iPSC media and reagents are manufactured in conformity with GMP for medical devices, 21 CFR Part 820, and follow USP<1043> and Ph Eur 5.2.12, and the manufacturing sites are FDA-registered and ISO 13485- and/or ISO 9001-certified. The investment in rigorous product manufacturing helps ensure the consistency, reliability, and high quality of iPSC therapy reagents in combination with the ability to scale up and accommodate changing needs.
  • Quality control testing and regulatory documentation—CTS iPSC media and reagents help minimize the risk of contamination and variability in research with extensive safety testing, including sterility, endotoxin, and mycoplasma. Full traceability for raw materials is also available, including Drug Master Files, Regulatory Support Files, and Certificates of Origin.
  • Proven use for a seamless transition from research to clinic—An extensive selection of serum-free, xeno-free, or animal origin-free formulations with cell therapy specific intended use statements and complementary research use reagents. Our CTS products have been used in over 200 clinical trials as well as in commercially approved cell therapies including FDA-approved CAR-T therapies and the first FDA-approved therapeutic cancer vaccine.
     


Explore CTS iPSC media and reagents

GMP iPSC Reprogramming kit

CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit

The first off-the-shelf reprogramming system manufactured in accordance with GMP requirements, offering high-efficiency Sendai delivery of reprogramming factors.

  • Xeno-free workflow—generate iPSC lines from both fibroblasts and blood for clinical research
  • Regulatory support—Traceability documentation, such as Certificate of Analysis and Certificate of Origin, available to support regulatory filing
  • Designed with transition in mind—complimentary research-use CytoTune iPS 2.0 Sendai Reprogramming Kit helps facilitate a seamless translation to the clinic

See data

CTS HiFi Cas9 Protein

Regulatory bodies like the FDA recognize the safety concerns of off-target cleavage events when using CRISPR-Cas9, particularly for therapeutics applications. To address these safety risks, there is a developed CTS HiFi Cas9 Protein, a GMP-manufactured high-fidelity Cas9 protein designed to significantly reduce off-target events while maintaining high editing efficiency.

  • Significantly reduce off-target effects—>90% reduction in a broad range of cell types including primary immune cells
  • Equivalent editing efficiency—Achieve the same high editing efficiency as CTS TrueCut Cas9 Protein, our wild-type protein designed for exceptional on-target editing efficiency
  • Improved knock-in efficiency—Also offered in CTS HiFi Cas9 and CTS Xenon Genome Editing Buffer Kit to offer 2X improved knock-in efficiency

See data

Order CTS HiFi Cas9 Protein

CTS TrueCut Cas9 Protein

Order CTS TrueCut Cas9 Protein

iPSC media for cell therapy

CTS Essential 8 Medium

Based on the widely cited Essential 8 Medium, Gibco CTS Essential 8 Medium was the first globally available human pluripotent stem cell (hPSC) culture medium with components not directly derived from animals.

  • Supports long-term iPSC culture—Maintains iPSC marker expression and a normal karyotype over multiple passages
  • Maintains differentiation potential—PSCs cultured in CTS Essential 8 Medium are able to generate cells of all three germ layers
  • Helps reduce risk—Based on the same fully defined and highly consistent eight-component formulation as research-use Essential 8 Medium but without components directly of animal or human origin

See data

Order CTS Essential 8 Medium

CTS KnockOut SR XenoFree Medium

Order CTS KnockOut SR XenoFree Medium


iPSC reagents for cell therapy

CTS Vitronectin (VTN-N) Recombinant Human Protein

Order CTS VTN-N Recombinant Human Protein

CTS Versene Solution

Order CTS Versene Solution

CTS KnockOut DMEM and CTS KnockOut DMEM/F12

Order CTS KnockOut DMEM and CTS KnockOut DMEM/F12

CTS RevitaCell Supplement

Gibco CTS RevitaCell Supplement (100X) is an animal-origin-free, chemically defined supplement used with PSCs for post-thaw recovery or in combination with CTS Essential 8 Medium for single cell passaging.

  • Improved cell survival—Provides high cell viability post-single cell passaging and post-thaw
  • Contains higher specificity inhibitor—Includes a higher specificity ROCK inhibitor, coupled with antioxidants and free radical scavengers to minimize impact of stress on cells
  • Offers consistency—Formulated using animal-origin free and chemically defined materials

Order CTS RevitaCell Supplement

CTS PSC Cryopreservation Kit

Order CTS PSC Cryopreservation Kit

CTS Essential 6 Medium

Order CTS Essential 6 Medium

CTS B-27 Supplement XenoFree

Product photo

Gibco B-27 Supplement has been the trusted standard for a variety of neuronal culture applications with citations in more than 11,000 publications. The CTS™ product offers proven serum-free supplementation for the growth, expansion, differentiation, and maintenance of PSC-derived cells.

  • Applicable to numerous cell types - neural stem cells, neurons, and cardiomyocytes
  • Three varieties available - full version, minus vitamin A, and minus insulin
  • Equivalent performance - same performance as the trusted research‑use Gibco B‑27 Supplement XenoFree

Order CTS B-27 Supplement XenoFree

Gibco PeproTech growth factors and cytokines for iPSC therapy

GMP iPSC Reprogramming kit

CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit

The first off-the-shelf reprogramming system manufactured in accordance with GMP requirements, offering high-efficiency Sendai delivery of reprogramming factors.

  • Xeno-free workflow—generate iPSC lines from both fibroblasts and blood for clinical research
  • Regulatory support—Traceability documentation, such as Certificate of Analysis and Certificate of Origin, available to support regulatory filing
  • Designed with transition in mind—complimentary research-use CytoTune iPS 2.0 Sendai Reprogramming Kit helps facilitate a seamless translation to the clinic

See data

CTS HiFi Cas9 Protein

Regulatory bodies like the FDA recognize the safety concerns of off-target cleavage events when using CRISPR-Cas9, particularly for therapeutics applications. To address these safety risks, there is a developed CTS HiFi Cas9 Protein, a GMP-manufactured high-fidelity Cas9 protein designed to significantly reduce off-target events while maintaining high editing efficiency.

  • Significantly reduce off-target effects—>90% reduction in a broad range of cell types including primary immune cells
  • Equivalent editing efficiency—Achieve the same high editing efficiency as CTS TrueCut Cas9 Protein, our wild-type protein designed for exceptional on-target editing efficiency
  • Improved knock-in efficiency—Also offered in CTS HiFi Cas9 and CTS Xenon Genome Editing Buffer Kit to offer 2X improved knock-in efficiency

See data

Order CTS HiFi Cas9 Protein

CTS TrueCut Cas9 Protein

Order CTS TrueCut Cas9 Protein

iPSC media for cell therapy

CTS Essential 8 Medium

Based on the widely cited Essential 8 Medium, Gibco CTS Essential 8 Medium was the first globally available human pluripotent stem cell (hPSC) culture medium with components not directly derived from animals.

  • Supports long-term iPSC culture—Maintains iPSC marker expression and a normal karyotype over multiple passages
  • Maintains differentiation potential—PSCs cultured in CTS Essential 8 Medium are able to generate cells of all three germ layers
  • Helps reduce risk—Based on the same fully defined and highly consistent eight-component formulation as research-use Essential 8 Medium but without components directly of animal or human origin

See data

Order CTS Essential 8 Medium

CTS KnockOut SR XenoFree Medium

Order CTS KnockOut SR XenoFree Medium


iPSC reagents for cell therapy

CTS Vitronectin (VTN-N) Recombinant Human Protein

Order CTS VTN-N Recombinant Human Protein

CTS Versene Solution

Order CTS Versene Solution

CTS KnockOut DMEM and CTS KnockOut DMEM/F12

Order CTS KnockOut DMEM and CTS KnockOut DMEM/F12

CTS RevitaCell Supplement

Gibco CTS RevitaCell Supplement (100X) is an animal-origin-free, chemically defined supplement used with PSCs for post-thaw recovery or in combination with CTS Essential 8 Medium for single cell passaging.

  • Improved cell survival—Provides high cell viability post-single cell passaging and post-thaw
  • Contains higher specificity inhibitor—Includes a higher specificity ROCK inhibitor, coupled with antioxidants and free radical scavengers to minimize impact of stress on cells
  • Offers consistency—Formulated using animal-origin free and chemically defined materials

Order CTS RevitaCell Supplement

CTS PSC Cryopreservation Kit

Order CTS PSC Cryopreservation Kit

CTS Essential 6 Medium

Order CTS Essential 6 Medium

CTS B-27 Supplement XenoFree

Product photo

Gibco B-27 Supplement has been the trusted standard for a variety of neuronal culture applications with citations in more than 11,000 publications. The CTS™ product offers proven serum-free supplementation for the growth, expansion, differentiation, and maintenance of PSC-derived cells.

  • Applicable to numerous cell types - neural stem cells, neurons, and cardiomyocytes
  • Three varieties available - full version, minus vitamin A, and minus insulin
  • Equivalent performance - same performance as the trusted research‑use Gibco B‑27 Supplement XenoFree

Order CTS B-27 Supplement XenoFree

Gibco PeproTech growth factors and cytokines for iPSC therapy


Custom iPSC media

We offer full customization options to help meet your unique specifications for any project. Flexibility is yours in creating your own Gibco custom cell culture medium.

  • Add or remove components
  • Change a concentration
  • Switch packaging
  • Select from cGMP or non-GMP
  • Choose QC tests

Learn more

product photo

Gibco CTS Rotea Counterflow Centrifugation System

  • Process flexibility
  • High cell recovery and viability
  • Low output volumes
  • Research through commercial manufacturing
     

Learn more

product photo

Gibco CTS Xenon Electroporation System

  • High speed and large volume
  • Scalable, proven performance and viability
  • Process flexibility
  • Efficient nonviral transfection
  • Closed-system processing

Learn more

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Intended use of the products mentioned on this page vary. For specific intended use statements please refer to the product label.