Induced Pluripotent Stem Cell (iPSC) Media and Reagents
for Cell Therapy

The first off-the-shelf reprogramming system manufactured in accordance with GMP requirements, offering high-efficiency Sendai delivery of reprogramming factors.

• Xeno-free workflow—generate iPSC lines from both fibroblasts and blood for clinical research
• Regulatory support—traceability documentation, such as Certificate of Analysis and Certificate of Origin, available to support your regulatory filing
• Designed with transition in mind—complimentary research-use CytoTune iPS 2.0 Sendai Reprogramming Kit helps facilitate a seamless translation to the clinic

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Based on the widely cited Essential 8 Medium, Gibco CTS Essential 8 Medium is the first globally available human pluripotent stem cell (hPSC) culture medium with components not directly derived from animals.

• Supports long-term iPSC culture—maintains iPSC marker expression and a normal karyotype over multiple passages
• Maintains differentiation potential—PSCs cultured in CTS Essential 8 Medium are able to generate cells of all three germ layers
• Helps reduce risk—based on the same fully defined and highly consistent eight-component formulation as RUO Essential 8 Medium but without components directly of animal or human origin

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Gibco CTS Essential 6 Medium is a xeno-free, feeder-free medium which supports the spontaneous or directed differentiation of human pluripotent stem cells (hPSCs) and the reprogramming of somatic cells.

• Provides a flexible format—suits different applications and various cell types
• Simplifies the transition from bench to clinic—contains the same six essential components as RUO Essential 6 Medium, and with its cGMP manufacturing process, this formulation helps minimize variability
• Supports differentiation and reprogramming—does not contain bFGF or TGFbeta

Designed in the laboratory of James Thomson, Gibco CTS Vitronectin (VTN-N) Recombinant Human Protein is a defined matrix for feeder-free culture of iPSCs and is intended for use with the CTS Essential 8 culture system.

• Animal origin–free—microbial expression recombinant matrix helps reduce variability and viral contamination risk in iPSC cultures
• Optimized and defined surface—VTN-N variant has been shown to support human iPSC attachment and survival better than wild type vitronectin
• Supports pluripotency—able to maintain pluripotency, normal growth characteristics, trilineage differentiation potential and normal karyotype after extended culture

Gibco CTS RevitaCell Supplement (100X) is an animal-origin-free, chemically defined supplement used with PSCs for post-thaw recovery or in combination with CTS Essential 8 Medium for single cell passaging. 

• Improved cell survival—provides high cell viability post-single cell passaging and post-thaw
• Contains higher specificity inhibitor—includes a higher specificity ROCK inhibitor, coupled with antioxidants and free radical scavengers to minimize impact of stress on cells
• Offers consistency—formulated using animal-origin free and chemically defined materials

Gibco CTS Versene is a gentle non-enzymatic cell dissociation reagent for routine clump passaging of PSCs while maintaining viability over multiple passages.

• Maintain viability over multiple passages—PSCs passaged with this cGMP manufactured EDTA dissociation reagent maintain viability
• Seamless transition from bench to clinic—same formulation as RUO Versene Solution but with additional characterization and quality control testing

The Gibco CTS PSC Cryopreservation Kit, which includes CTS PSC Cryomedium and CTS RevitaCell Supplement, minimizes the loss of cell viability and maximizes post-thaw recovery of PSCs.

• Provides efficient recovery—routinely achieves higher viability than traditional methods of PSC cryopreservation
• Offers consistency—chemically defined media maintains normal morphology, pluripotency, and karyotype of PSCs
• Reduces variability—optimized reagents minimize the causes of genetic instability of PSCs

Gibco CTS PSC Cryomedium is a xeno-free solution for the cryopreservation of PSCs. It also helps minimize loss of cell viability and maximize post-thaw recovery as part of the CTS PSC Cryopreservation Kit.

• Delivers higher recovery efficiency—efficient recovery and higher viability than traditional methods of cryopreservation
• Provides versatility—compatible with clump and singe cell passaged PSCs cultured in a variety of media systems
• Offers ease of use—provided as a ready-to-use , complete solution with no required additions of enhancing reagents

Gibco CTS KnockOut SR XenoFree Medium is a defined, xeno-free serum replacement based on the traditional KnockOut Serum Replacement, which has been cited in more than 2,000 publications and trusted for over 20 years.

• Supports pluripotency—able to maintain the pluripotency, normal morphology, and karyotype of hPSCs
• Versatile—can also be used for cryopreservation, derivation, and differentiation of iPSCs
• Designed for your applications—a first-to-market, xeno-free iPSC product

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Your choice of chemically defined human- and animal origin-free basal media for pluripotent stem cell culture. Based on traditional DMEM and DMEM/F12 formulations, these basal media are:

• Optimized—lower osmolarity as compared to traditional formulations improve maintenance of undifferentiated iPSCs
• Versatile—have been shown to support expansion and differentiation of a variety of stem cells, including iPSCs
• Tried and tested—cited in many publications, often used with CTS KnockOut SR XenoFree supplement