Dynabeads Sequencing Cleanup Kit

Streamline your high throughout sequencing workflow

The Invitrogen Dynabeads Sequencing Cleanup Kit is designed to enhance the efficiency and reliability of your capillary electrophoresis (CE) applications, including all Sanger sequencing-based protocols. It leverages the power of uniform, superparamagnetic beads to ensure rapid and effective purification of dye-terminator sequencing reactions.

Key features include:

  • Automation compatible—fully compatible with 96- and 384-well plate workflows
  • No centrifugation or filtration—simplifies the process, reducing the risk of sample loss
  • Rapid protocol—complete your cleanup in just 15 minutes with a straightforward, one-tube protocol
  • High-quality beads—over 30 years of experience enables exceptional quality and reliability
  • Efficient dye terminator removal—achieve dye blob-free sequencing data
  • Custom solutions—tailored to address specific research needs
  • High phred 20 bases—generates sequences with a high number of Phred 20 bases for high data quality

How the Dynabeads Sequencing Cleanup Kit works

The Dynabeads magnetic bead separation protocol consists of three simple steps: binding, washing, and elution. This streamlined process does not require centrifugation or columns, making sample processing straightforward and efficient. Additionally, the protocol can be automated using KingFisher instruments or other automation platforms available on the market.

The beads you choose matter

Dynabeads magnetic beads are exceptionally uniform and monodispersed, supporting high reproducibility and minimal nonspecific binding. Unlike other magnetic particles that may trap impurities due to variable shapes and sizes, Dynabeads provide a defined surface for consistent results.

Key features and benefits include:

  • Magnetic bead separation—gentle on nucleic acids, preserving sample integrity without the need for columns or centrifugation
  • Uniform bead size—promotes consistent binding properties and highly reproducible results
  • Spherical shape and defined surface chemistry—minimizes aggregation and non-specific binding
  • Nonporous bead surface—reduces non-specific binding and quantity of reagents required for separation
  • Supermagnetic particles—allows beads to be magnetic only in a magnetic field, with no residual magnetism once removed

Figure 1. Dynabeads magnetic beads vs. magnetic particles from other suppliers. (A) Dynabeads magnetic beads are exceptionally uniform, monodispersed superparamagnetic beads, manufactured with highly controlled product quality to help ensure exceptional reproducibility. (B-D) Magnetic particles from other suppliers often have variable shapes and sizes that trap impurities, which can result in lower reproducibility and increased nonspecific binding.

Ordering information

For Research Use Only. Not for use in diagnostic procedures.